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Glucolase electrode based on magnetic carbon nano-tube and preparation method thereof

A technology of magnetic carbon nanotubes and glucose enzymes, applied in the field of biosensors, achieves the effects of simple preparation, wide detection concentration range, and cost reduction

Inactive Publication Date: 2008-12-24
FUDAN UNIV
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Problems solved by technology

[0004] The present invention adopts the chemical co-precipitation method to modify ferric oxide and chitosan on the surface of carbon nanotubes, fix glucose oxidase by glutaraldehyde, and obtain carbon nanotubes / ferric oxide / shells immobilized with glucose oxidase Neither the polysaccharide nano-magnetic composite material nor the new glucose enzyme electrode prepared has been reported in the literature

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[0025] Further describe the present invention below by embodiment and accompanying drawing:

[0026] 1. Renewable glucose enzyme electrode based on magnetic carbon nanotubes and its fabrication

[0027] Dissolve 0.2 g of chitosan with 2 g of glacial acetic acid, and set the volume to 200 ml, wherein the concentration of chitosan is 1 mg / mL, and the concentration of acetic acid is 1 mg / mL. In this solution was added 1.7 grams (4.33mmol) ferrous sulfate (II) ammonium ((NH 4 ) 2 Fe(SO 4 )·6H 2 O) and 2.51 grams (8.66 mmol) of iron (III) ammonium sulfate (NH 4 Fe(SO 4 ) 2 12H 20), heated and dissolved at 50° C., then added 1 gram of multi-walled carbon nanotubes (provided by Shenzhen Nanometer Harbor Co., Ltd.), ultrasonically dispersed for 15 minutes, and added 10 milliliters of 10M ammonia water dropwise while stirring, so that the final pH value of the solution was 11- 12. Then the mixed solution was heated in a water bath at 50° C. for 30 minutes, and the magnet was pl...

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Abstract

The invention belongs to the field of biological sensor technique, in particular to a glucolase electrode and a preparation method thereof on the basis of a magnetic carbon nano-tube. The preparation method of the invention disperses the carbon nano-tube ultrasonic into a chitosan acid aqueous solution containing ferric ions and ferrous ions so as to prepare the three-composition nanometer magnetic composite material which consists of the carbon nano-tube, ferroferric oxide and chitosan; the three-composition nanometer magnetic composite material is dispersed into glucose oxidase aqueous solution, adsorbs the glucolase and is dispersed into glutaraldehyde aqueous solution; by primary amino-group reaction crosslinking, the glucose oxidase is fixed on the surface of the magnetic composite material, thus obtaining the updatable glucolase electrode based on the magnetic carbon nano-tube; the glucolase electrode provided by the invention has high response speed, wide detection consistency range, simple and convenient electrode processing and simple and convenient preparation of the immobilized enzyme magnetic material; when the enzyme is deactivated, the electrode is regenerated by updating the enzyme material of the electrode surface based on the magnetic carbon nano-tube, therefore, the consumption of the electrode material is greatly reduced, the resource is saved and the cost of enzymatic determination is reduced.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to a glucose enzyme electrode based on magnetic carbon nanotubes and a preparation method thereof. Background technique [0002] Since the first report of carbon nanotubes by Iijima[1] in Japan in 1991, the material has attracted widespread attention in related fields at home and abroad for its unique structure, excellent electrical conductivity, high mechanical strength, chemical stability and catalytic activity. It has obtained preliminary applications in many fields such as power supply, electromagnetic shielding, nanoelectronic devices, super composite materials, hydrogen storage materials, catalyst carriers, etc., and has aroused great interest in the research fields of physics, chemistry and materials in the world. In recent years, the research and development of carbon nanotubes at home and abroad have been extremely active, and major breakthroughs have been co...

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Application Information

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IPC IPC(8): G01N27/327
Inventor 陈刚陈挚张鲁雁
Owner FUDAN UNIV
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