Glucolase electrode based on magnetic carbon nano-tube and preparation method thereof

Abstract

The invention belongs to the field of biological sensor technique, in particular to a glucolase electrode and a preparation method thereof on the basis of a magnetic carbon nano-tube. The preparation method of the invention disperses the carbon nano-tube ultrasonic into a chitosan acid aqueous solution containing ferric ions and ferrous ions so as to prepare the three-composition nanometer magnetic composite material which consists of the carbon nano-tube, ferroferric oxide and chitosan; the three-composition nanometer magnetic composite material is dispersed into glucose oxidase aqueous solution, adsorbs the glucolase and is dispersed into glutaraldehyde aqueous solution; by primary amino-group reaction crosslinking, the glucose oxidase is fixed on the surface of the magnetic composite material, thus obtaining the updatable glucolase electrode based on the magnetic carbon nano-tube; the glucolase electrode provided by the invention has high response speed, wide detection consistency range, simple and convenient electrode processing and simple and convenient preparation of the immobilized enzyme magnetic material; when the enzyme is deactivated, the electrode is regenerated by updating the enzyme material of the electrode surface based on the magnetic carbon nano-tube, therefore, the consumption of the electrode material is greatly reduced, the resource is saved and the cost of enzymatic determination is reduced.

Description

technical field

[0001] The invention belongs to the technical field of biosensors, and in particular relates to a glucose enzyme electrode based on magnetic carbon nanotubes and a preparation method thereof. Background technique

[0002] Since the first report of carbon nanotubes by Iijima[1] in Japan in 1991, the material has attracted widespread attention in related fields at home and abroad for its unique structure, excellent electrical conductivity, high mechanical strength, chemical stability and catalytic activity. It has obtained preliminary applications in many fields such as power supply, electromagnetic shielding, nanoelectronic devices, super composite materials, hydrogen storage materials, catalyst carriers, etc., and has aroused great interest in the research fields of physics, chemistry and materials in the world. In recent years, the research and development of carbon nanotubes at home and abroad have been extremely active, and major breakthroughs have been co...

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