Enterobacteria recombinant strain and use thereof

Abstract

The invention discloses a recombination strain for an enterobacterium and application of the recombination strain for the enterobacterium. The recombination strain provided by the invention is a recombination strain obtained after deactivation of a pyruvate formate-lyase gene in the enterobacterium. As shown by experiments, the amount of by-product CO2 produced during the fermentation process of the recombination strain is obviously reduced and the amount of target products is obviously improved compared with the prior art. For example, the amount of lactic acid, the amount of succinic acid and the amount of 2, 3-butanediol produced by fermentation of klebsiella oxytoca are respectively improved by 30 percent, 9 percent and 23 percent compared with the prior art, and the amount of the CO2 produced is only 8.04 percent compared with the prior art. The recombination strain helps to release the pressure of the greenhouse effect, has important function in the actual industrial production, not only can greatly reduce the yield of the CO2 but also can improve the conversion rate of converting substrates into products, and has wide application prospect.

Description

technical field [0001] The invention relates to a recombinant bacterium and its application, in particular to a recombinant bacterium of Enterobacter and its application. Background technique [0002] In the fermentation industry, when the substrate generates the target product under the action of microorganisms or enzymes, by-products are often produced, CO 2 It is one of the most common by-products in the fermentation industry. During the anaerobic fermentation of sugars by strains of Enterobacteriaceae, sugars are converted into phosphoenolpyruvate through a series of reactions, a part of phosphoenolpyruvate is converted into succinic acid, and the other part is converted into pyruvic acid, pyruvic acid There are three outlets: 1. converted into lactic acid under the catalysis of lactate dehydrogenase; ) catalyzed by α-acetolactate and 3-hydroxybutanone into 2,3-butanediol in turn; 3. decomposed into acetyl CoA and formic acid under the catalysis of pyruvate formate lya...

AI Technical Summary

Problems solved by technology

CO 2 The generation of the substrate not only caused the reduction of the conversion rate of the substrate into the product, but also exacerbated the already increasingly serious greenhouse effect.

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