Gene chip for detecting Mycotoruloides

A technology of gene chip and candida, applied in the field of gene chip, to achieve high sensitivity and high specificity

Active Publication Date: 2009-01-21
LANZHOU BAIYUAN GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Genotyping and homology analysis of Candida using gene

Method used

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  • Gene chip for detecting Mycotoruloides
  • Gene chip for detecting Mycotoruloides
  • Gene chip for detecting Mycotoruloides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Materials and methods

[0019] 1.1 Materials

[0020] 1.1.1 Reagents

[0021] Taq DNA polymerase was purchased from Beijing Biotech, the PCR product purification kit was purchased from v-gene Biotechnology Co., Ltd., dNTP was purchased from Promega Company, RNAsin was purchased from Dalian Bao Biological Engineering Co., Ltd., and salmon sperm DNA was purchased from Sigama Company.

[0022] 1.1.2 Strains

[0023] Candida albicans, Candida tropicalis, Candida plabrata, Candida parapsilosis, Candida krusei, Candida kefyr, Both Candida stellatoidea and C.dubliniensis were clinical isolates, which were double-identified by CHROMagar chromogenic medium and PCR product sequence determination.

[0024] 1.1.3 Instruments and equipment

[0025] Instruments and equipment: PCR instrument (Hangzhou Langji MG96+); spotter; hybridization instrument; scanner.

[0026] 2. Primer Design

[0027] Candida albicans, Candida tropicalis, Candida glabrata, Candida parapsilosis, Candi...

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Abstract

The invention provides a gene chip used for detecting candida, relating to the biological field, and aiming to solve the problems that because mycotic infection is hard to diagnose, and the prior diagnosis method is relatively laggard and is always lagged behind clinical therapy, many patients can not get correct and timely diagnosis and lose therapy opportunity. The gene chip is characterized in that the chip comprises: (1) an oligonucleotide probe for detecting different types of candidas and a quality control oligonucleotide probe, and (2) a probe array formed by the oligonucleotide probes fixed on a carrier material through arm molecule. Moreover, the gene chip has the advantages of quick and accurate detection of candida infectious diseases as well as high sensitivity and specificity.

Description

technical field [0001] The invention relates to the biological field, in particular to a gene chip. Background technique [0002] Fungal infection is a serious complication of clinically critically ill patients, and fungal infection and its treatment have attracted more and more attention. Candida occupies the first place among fungal infections, among which Candida albicans is the most common (60%-70%), followed by Candida tropicalis (10%). The most common specimens for isolating fungi are respiratory tract sputum specimens and oral saliva specimens, followed by blood, urine, birth canal or wound secretions, etc. [0003] The traditional identification method of Candida species is mainly based on morphology, by observing colonies and microscopic characteristics after cultivation. Due to the difficulty in the diagnosis of fungal infection, the method is relatively backward, and often lags behind the clinical treatment, resulting in many patients not being diagnosed correct...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 车团结徐进章何祥一
Owner LANZHOU BAIYUAN GENE TECH
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