Keratin degrading bacteria NJY1

A technology of keratin-degrading bacteria and strains, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve problems such as the shortage of feed protein resources, achieve the effects of promoting sustainable development, broad application prospects, and protecting the environment

Inactive Publication Date: 2009-02-25
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a high-efficiency keratin-degrading bacterial strain for the development of new protein feedstuffs in view of the serious shortage of feed protein resources in production practice

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Efficient strain isolation

[0016] Collect 2 g of samples from special environments such as the soil where feathers are accumulated, add 10 mL of sterile saline, shake for 1 min, let stand for 5 min, absorb the supernatant to inoculate the nutrient broth medium, and incubate at 37 °C for 48 h.

[0017] Streak inoculation of the above-mentioned cultured strains on a feather meal medium plate, and culture at 37° C. for 24 hours. The formula of the feather meal culture medium is that 1L of distilled water contains: 5.0g sodium chloride, 20.0g feather meal, and 20.0g agar powder. Heat to dissolve, adjust pH to 7.5, and autoclave at 121°C for 15 minutes.

[0018] Purify the bacteria that produce larger hydrolysis circles in the above medium. A bacterium strain with high ability to hydrolyze keratin was obtained and named: NJY1. The strain NJY1 is classified and named: Bacillus thuringiensis, and was deposited in the General Microorganism Center of China Com...

Embodiment 2

[0019] Embodiment 2: the expansion culture of above-mentioned bacterial strain NJY1

[0020] The formula of the seed medium is that 1L of distilled water contains: 6.0g of peptone, 4.0g of beef extract, 2.0g of yeast extract, and 5.0g of sodium chloride. Heat to dissolve, adjust pH to 7.5, and autoclave at 121°C for 15 minutes.

[0021] The bacterial strain NJY1 obtained above was inoculated into a 250 mL Erlenmeyer flask containing 50 mL of seed medium, and cultivated on a shaker at a speed of 120 r / min for 24 hours at a temperature of 37 ° C to obtain a liquid culture of NJY1. The number of cells was 4×10 11 CFU / mL.

Embodiment 3

[0022] Embodiment 3: Utilize bacterial strain NJY1 to ferment and degrade feather in feather fermentation medium

[0023] Take 1 mL of the NJY1 liquid culture obtained in Example 2, inoculate it into a 500 mL Erlenmeyer flask containing 100 mL of feather fermentation medium, and cultivate it on a shaker at a speed of 180 r / min for 3 days at a temperature of 37° C. The formula of the feather fermentation medium is, 1L distilled water contains: 0.01g calcium chloride, 0.1g magnesium sulfate, 0.5g sodium chloride, 0.3g dipotassium hydrogen phosphate, 0.4g potassium dihydrogen phosphate, 160 complete feathers, adjusted pH8.0, autoclave at 121°C for 15 minutes. As the feathers in the culture medium increased with time, the feather sprigs gradually fell off, and then the feather stalks gradually broke. After 3 days, the feathers were completely degraded, and the culture medium became a uniform suspension.

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Abstract

The invention relates to a keratin degradating bacterium NJY1 which belongs to the high technical field of biology. The strain NJY1 is named in classification as Bacillus thuringiensis, and the culture collection number is CGMCC NO.2494. The strain is inoculated in a seed culture medium and cultured at the temperature of 37 DEG C and at the rate of 120r/min for 24h to obtain a liquid culture of the NJY1. The strain produces transparent hydrolysis rings on a feather meal culture medium, the feather ferments and is degraded in a feather fermentation culture medium, and the degradation rate of the keratin in a keratin fermentation culture medium is 72.5%. The keratin degradation bacterium degrades the keratin by the method of microbial fermentation, which is suitable for producing high-quality protein feed. The keratin degradation bacterium can be used for developing and utilizing waste feather resources of slaughtered livestock and poultry, particularly transforming the feather protein into feed, so that waste can be changed into valuables, environment is protected, sustainable development can be promoted, and the application prospect is wide.

Description

technical field [0001] The invention relates to a keratin-degrading bacterium NJY1, which belongs to the high-tech field of biology, uses microbial fermentation to degrade keratin, and is suitable for the production of high-quality protein feed. Background technique [0002] In recent years, with the rapid development of my country's animal husbandry, the demand for feed protein is also increasing. At present, my country mainly relies on imported fishmeal to solve the shortage of protein resources, so how to develop new protein resources is a current research hotspot. . my country's feather resources are extremely rich, with an annual output of hundreds of thousands of tons. The discarding of a large number of feathers not only causes a huge waste of resources, but also pollutes the environment. The protein content of poultry feathers is high, generally 75-90%, and the amino acid types are relatively complete. Except for the slightly lower content of lysine and methionine, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/00C12R1/07
Inventor 杨德吉姚大伟王政陶艳华郭士兵
Owner NANJING AGRICULTURAL UNIVERSITY
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