Vaccine for in ovo inoculation

A technology for vaccines and poultry, applied to veterinary vaccines, vaccines, medical preparations containing active ingredients, etc., can solve problems such as taking a very long time

Inactive Publication Date: 2009-02-25
JURIDICAL FOUND THE CHEMO SERO THERAPEUTIC RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the above-mentioned mutant strain is used as a vaccine, it cannot be completely denied that it may have adverse effects on poultry and humans who ingest the poultry
Therefore, it is not widely available yet, and its evaluation is expected to take a very long time

Method used

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  • Vaccine for in ovo inoculation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] "Example 1: Production of ND vaccine for in ovo vaccination with added virus adsorbent"

[0047] Newcastle disease (ND) vaccines for inoculation in ovo were prepared by adding potassium alum, aluminum hydroxide gel, hydroxyapatite, and silica gel as virus adsorbents. Potassium alum and aluminum hydroxide gels were prepared using physiological saline according to conventional methods, so that the aluminum content was 2 mg / ml, and hydroxyapatite was prepared using physiological saline to be 200 mg / ml. Each 10ml of potassium alum, aluminum hydroxide gel and hydroxyapatite solution was prepared with 10ml of physiological saline to make 10 5.3 EID 50 / ml of the ND virus attenuated strain D26 strain virus solution was mixed, and stirred overnight with a stirrer at 4°C. In addition, 1 g of powdered silica gel and 1 ml of saline were prepared as 10 6.3 EID 50 / ml of ND virus attenuated strain D26 strain virus solution was mixed, added physiological saline to a total of 20ml...

Embodiment 2

[0051] "Example 2: Safety of ND Vaccine for In-ovo Inoculation with Added Virus Adsorbent in SPF Eggs"

[0052] Using the ND vaccine for in ovo inoculation with the addition of potassium alum and aluminum hydroxide gel prepared according to the method described in Example 1, according to the method reported by Sharma et al. In ovo inoculation of SPF 18-day-old developing chicken eggs (groups 1 and 2). A test group in which no virus adsorbent was added but only virus was set as a control (group 3). As a result, as shown in Table 2, there was a significant difference in hatchability between the ND vaccine group for in-ovo inoculation to which the virus adsorbent was added and the non-addition group (using Fisher's direct probability calculation method to test the significant difference from the non-addition group). ). In addition, there was also a significant difference in the survival rate at the second week after hatching between the ND vaccine group for in ovo inoculation t...

Embodiment 3

[0059] "Example 3: Effectiveness of ND vaccine for in ovo vaccination with added virus adsorbent in SPF eggs"

[0060] In order to evaluate the effectiveness of the test group tested in Example 2, serum was collected at the third week after hatching, and red blood cells were administered using ND virus hemagglutinin (Institute of Chemistry and Serotherapy, Inc.) as described in the attached instruction manual. Agglutination inhibition test (HI test). It is known that generally the HI antibody titer of more than 5 times can defend against the attack of the ND virus virulent strain Sato strain, so the HI antibody titer of the ND vaccine group in ovo inoculation that has added the virus adsorbent is sufficiently higher than the defense level (Table 4).

[0061] [Table 4]

[0062]

[0063] 1) : Antibody positive rate (%) is shown in brackets. It is positive when it is more than 5 times.

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Abstract

It is intended to provide a vaccine for in ovo inoculation which is effective in the prevention of all poultry viral diseases. It is a poultry vaccine for in ovo inoculation with high effectiveness also from a safety aspect in which the pathogenicity of a virus whose practical application as a vaccine for in ovo inoculation was difficult in the past against embryo is reduced by adsorbing and retaining the virus onto an virus adsorbing agent thereby to make the virus growth in ovo after inoculation slow and the improvement in a decrease in the hatching rate and the alleviation of severe clinical symptoms after hatching can be realized. As the virus adsorbing agent, an aluminum compound such as an aluminum hydroxide gel or aluminum potassium sulfate is used.

Description

technical field [0001] The present invention relates to poultry vaccines for in ovo vaccination. Specifically, it relates to a poultry vaccine for inoculation in ovo, characterized in that a live virus is adsorbed and held on a virus adsorbent. Background technique [0002] All over the world, commercially raised poultry are vaccinated for the prevention of infection or disease from various pathogens. Common viruses that cause diseases in poultry include, for example, Marek's disease virus, fowlpox virus, infectious supracavitary disease virus, Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, and avian encephalomyelitis Viruses, Chicken Anemia Virus, Avian Influenza Virus, Avian Reovirus, Avian Leukosis Virus, Reticuloendotheliosis Virus, Avian Adenovirus, Avian Pneumovirus and Pigeon Pox Virus, etc. The diseases mentioned above have a great impact on the poultry industry. Vaccines are now used against many of the diseases mentione...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/39A61P31/12
CPCC12N2760/18134C12N2710/24034A61K39/12A61K2039/5254A61K2039/525A61K2039/552A61K2039/55505C12N2760/18334A61K2039/55A61P31/12
Inventor 太田秀幸江副伸介山崎宪一河合透
Owner JURIDICAL FOUND THE CHEMO SERO THERAPEUTIC RES INST
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