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Preparation method of DHA lipid nano-particles

A technology of lipid nanoparticles and lipid materials, which is applied in the directions of liposome delivery, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., can solve the problem of low efficiency and insufficient DHA synthesis to meet the needs of the Development requirements and other issues, to achieve the effect of simple and convenient preparation process, improved DHA stability, and high repeatability

Active Publication Date: 2009-03-18
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Generally, adults have the ability to synthesize DHA from linolenic acid precursors, but the efficiency of this conversion pathway is very low, and the amount of DHA synthesis is not enough to meet the needs of body development, so it must be supplemented from food

Method used

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  • Preparation method of DHA lipid nano-particles

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Experimental program
Comparison scheme
Effect test

Embodiment

[0022] Embodiment: a kind of preparation method of DHA lipid nanoparticle, comprises the following steps:

[0023] Step 1: Weigh the emulsifier and lipid material separately, the mass ratio of the two is 4:1~1:1, mix the emulsifier and lipid material and heat to the set temperature t, t=70°C, after melting Obtain a liquid oil phase;

[0024] Step 2: Weigh DHA, the mass ratio of DHA to lipid material is 3:1-1:2, add DHA into the liquid oil phase, and stir to obtain a homogeneous system;

[0025] Step 3: Add water at the same temperature as the set temperature t to the above system. The quality of the water is 7 to 30 times that of DHA. After mixing and stirring evenly, enter the preheating process to the set temperature t and set the pressure to 80MPa The high-pressure homogenizer circulates and homogenizes for 5 to 9 times, and water-soluble surfactants can be dissolved in the water;

[0026] Step 4: Cool the obtained high-temperature microemulsion to room temperature to obt...

Embodiment 1

[0031] 1. Weigh 6.48 grams of decaglycerol monolaurate, 4.32 grams of hexapolyglycerol monostearate, 1.2 grams of sucrose ester, and 3 grams of glyceryl monostearate, and put them into a beaker for heating in a 70°C water bath;

[0032] 2. After the substance in the beaker is completely melted, add 5 grams of DHA, heat and stir evenly;

[0033] 3. Add 80 grams of distilled water at 70°C to the above system and stir evenly. At the same time, turn on the high-pressure homogenizer and preheat it with distilled water at 70°C;

[0034] 4. Put the homogeneously stirred high-temperature emulsion system into the high-pressure homogenizer, adjust the homogenization pressure to 80MPa, and circulate the homogenization for 5 times under this pressure;

[0035] 5. Reduce to room temperature to obtain DHA lipid nanoparticle dispersion.

Embodiment 2

[0037] 1. Weigh 2.88 grams of decaglycerol monolaurate, 1.97 grams of hexapolyglycerol monostearate, 1.2 grams of sucrose ester, 1.5 grams of glyceryl monostearate, 1.5 grams of Camellia oleifera seed oil, and put them in a beaker at 70°C Water bath;

[0038] 2. After the substance in the beaker is completely melted, add 5 grams of DHA, heat and stir evenly;

[0039] 3. Add 86 grams of distilled water at 70°C to the above system and stir evenly. At the same time, turn on the high-pressure homogenizer and preheat it with distilled water at 70°C;

[0040] 4. Pour the uniformly stirred high-temperature emulsion system into a high-pressure homogenizer, adjust the homogenization pressure to 80MPa, and circulate the homogenization for 5 times under this pressure;

[0041] 5. Reduce to room temperature to obtain DHA lipid nanoparticle dispersion.

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Abstract

The invention discloses a preparation method of DHA lipid nanoparticles, the preparation method comprises the following steps: step one: an emulsifying agent and lipid material are respectively weighed, the emulsifying agent and the lipid material are heated to the set temperature after the mixing, a liquid oil phase is obtained after the melting; step two: DHA is weighed, the DHA is added in the liquid oil phase, a uniform system is obtained by stirring; step three: water with the temperature which is the same with the set temperature T is added in the system, then the system enters a high pressure homogenizer which is preheated to the set temperature T and at the set pressure of 80MPa after the even mixing and stirring; step four: the obtained high temperature micro-emulsion is cooled to the room temperature, and aqueous dispersion of DHA-NLC is obtained; the preparation method adopts the lipid nanoparticles to load DHA, thereby protecting the DHA from being oxidized and covering up the stinking smell of DHA oil; furthermore, the prepared DHA aqueous dispersion can be conveniently added in foods, thereby expanding the using range thereof.

Description

technical field [0001] The invention relates to a preparation method of DHA lipid nanoparticles. Background technique [0002] DHA is the abbreviation of docosahexaenoic acid (Docosahexaenoic Acid), which belongs to -3 series polyunsaturated fatty acids. DHA is an essential fatty acid for the human body, mainly found in the cerebral cortex and retina. DHA is closely related to the functions of the brain, especially information transmission, behavior, learning and vision. In addition, DHA also has certain medicinal value, such as treating cardiovascular diseases, improving symptoms of certain inflammatory and autoimmune diseases, and anticancer and antiviral effects. [0003] DHA is an important nutrient during the development of infant nerve cells; it has a lot to do with the responsiveness of infants during their growth, so DHA is called a smart fatty acid. From birth until three years of age, DHA accumulates in large quantities in human brain tissue. DHA is the most ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/202A61P3/02A61K47/26
Inventor 夏强徐弟
Owner PROYA COSMETICS
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