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Method for preparing rhodiola rosea liquid conversion leavening by microbial hybrid bacterial strain

A technology of mixed strains and rhodiola, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve strong utilization, reduce discharge, and reduce the consumption of raw medicinal materials

Inactive Publication Date: 2009-03-18
SICHUAN UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing Rhodiola liquid transformation fermentation products with microbial mixed strains, which can greatly improve The effective active ingredients in the original medicinal materials of Rhodiola can obtain more extraction products under the condition of using the same amount of original medicinal materials, so that the wild Tibetan medicinal materials can be fully utilized

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  • Method for preparing rhodiola rosea liquid conversion leavening by microbial hybrid bacterial strain

Examples

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Effect test

Embodiment 1

[0034]This embodiment is the preparation of Aspergillus niger and Aspergillus oryzae mycelia.

[0035] 1) Mash the natural wild Rhodiola rosea medicinal material, take 1g, add 5ml of sterile water, shake well, and then dilute 10, 100, 1000 and 10000 times with sterile water respectively. Take 0.1ml of each of the above dilutions, spread them on the separation medium consisting of 10g of glucose, 5g of peptone, 5g of yeast extract, 1g of potassium dihydrogen phosphate, 0.5g of ammonium sulfate, 20g of agar and 1000ml of water. Cultivate for 3 days, pick a single colony, and select a bacterial strain whose content of salidroside is increased after being transformed and fermented in the Rhodiola rosea medium and detected by high performance liquid chromatography (HPLC).

[0036] 2) first mix the Rhodiola rosea powder with a particle size of 80 meshes and water in 1:10 parts by weight, then add 0.2% yeast extract, 0.1% potassium dihydrogen phosphate and 0.05% sulfuric acid in part...

Embodiment 2

[0038] 100g Rhodiola rhizome raw material is pulverized to 60 mesh, then add 0.5g yeast extract, 0.5g potassium dihydrogen phosphate, 0.2g magnesium sulfate and 800mL water, stir and mix evenly, make it become Rhodiola rosea liquid water suspension, and use Steam was sterilized at 121° C. for 30 minutes; after cooling, inoculate the mixed mold mycelium of 80 g (wet weight) prepared by the method of Example 1, wherein the weight ratio of Aspergillus niger and Aspergillus oryzae 8:2, and then incubated and fermented at 30°C for 50 hours.

[0039] The obtained liquid converted fermented product was detected by high-performance liquid chromatography (HPLC), and the salidroside content was 1.3586%. Compared with the salidroside content of the original medicinal material detected under the same conditions as 0.7306%, the converted fermented product The content of salidroside can be increased by 85.96%.

Embodiment 3

[0041] 100g Rhodiola rosea rhizome raw material is crushed to 80 mesh, then add 1g yeast extract, 1g potassium dihydrogen phosphate, 0.3g magnesium sulfate and 900mL of water, stir and mix evenly, make it become Rhodiola rosea liquid water suspension, and use Steam was sterilized at 121° C. for 30 minutes; after cooling, inoculate the mixed mold mycelium of 90 g (wet weight) prepared by the method of Example 1, wherein the weight ratio of Aspergillus niger (Aspergillus niger) and Aspergillus oryzae (Aspergillus oryzae) 6:4, and then incubated and fermented at 30°C for 50 hours.

[0042] The obtained liquid converted fermented product is detected by high-performance liquid chromatography (HPLC), and the content of salidroside is 1.4125%. Compared with the salidroside content of the original medicinal material detected under the same conditions as 0.7306%, the converted fermented product The content of salidroside can be increased by 93.33%.

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Abstract

The invention provides a method for improving active compositions in a raw medicinal material of rhodiola by transforming and fermenting a mixed microorganism strain in a liquid water suspension of the raw medicinal material of the rhodiola. The method comprises the follow steps: the liquid water suspension of the rhodiola produced by rhodiola rootstalk powder is sterilized and then grafted with the mixed microorganism strain for liquid fermentation and cultivation. The transformation and fermentation by the method can ensure that the active compositions of the rhodiola are greatly improved than those in the raw medicinal material, and ensure that the content of the active compositions is highest and can be improved by more than 100 percent than those in the raw medicinal material, so the method can effectively utilize wild resources, can also reduce the discharge of pollutants such as drug residue and so on, realize the protective development of Chinese Tibetan medicine, and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of extracting effective active ingredients from Rhodiola rosea Tibetan traditional Chinese medicinal materials, and specifically relates to a method for preparing Rhodiola rosea liquid transformation fermented product by using microbial mixed strains, and the method makes effective active ingredients contained in the fermented product Greatly improve. Background technique [0002] Rhodiola refers to the genus Rhodiola in the Sedum family. There are more than 90 species in the world, mostly distributed in the alpine regions of the northern hemisphere, and most of them grow under mountain flowstones or shrubs at an altitude of 3500-5000 meters. There are 73 species found in our country, which are mainly distributed in the alpine and alpine regions of Tibet. In addition, Rhodiola resources are also distributed in some areas of Northeast, Northwest, and North China. [0003] The main medicinal part of Rhodiola ...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/685C12R1/69
Inventor 邓小晨
Owner SICHUAN UNIV
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