Musca domestic pupae natural antimicrobial peptide products and preparation method and use thereof

A natural antibacterial and housefly pupa technology, applied in the preparation method, application, and cosmetic preparations of peptides, can solve the problems of low yield of antibacterial peptides, difficulty in large-scale production and preparation, and long process cycle, and reach the distribution range Broad, not easy to drug resistance, broad antibacterial spectrum effect

Inactive Publication Date: 2009-03-25
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few research reports on the preparation technology of the housefly, which are only found in Hou et al. (Hou L X, Shi Y H, Zhai P, et al. Antibacterial activity and in vitro anti-tumor activity of the extract of the larvae of the housefly ( Muscadomestica).Journal of Ethnopharmacology, 2007,111(2):227-231.) and Lu Jie et al. 2): Reports from 106-112.)
And at present, the preparation process of housefly antimicrobial peptides is mostly in the stage of experimental exploration, the yield of antimicrobial peptides in the extraction process is low (less than 1%), the process cycle is long, and it is difficult to carry out large-scale production and preparation

Method used

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  • Musca domestic pupae natural antimicrobial peptide products and preparation method and use thereof
  • Musca domestic pupae natural antimicrobial peptide products and preparation method and use thereof
  • Musca domestic pupae natural antimicrobial peptide products and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Preparation of natural antimicrobial peptide products from housefly pupae

[0038] Specifically include the following steps:

[0039] (1) Weigh a certain amount of housefly pupae, wash with distilled water and dry, add the corresponding volume of 4°C pre-cooled 0.1M acetic acid solution according to the volume-to-weight ratio of 1ml:4g, homogenate, extract overnight at 4°C, and then place Heat treatment in a water bath at 90°C for 25 minutes, and then centrifuge at 4°C and 8000×g for 25 minutes to collect the supernatant, which is the crude extract of housefly pupae. After precipitation and drying (100°C), it is crushed into powder, which is one of the by-products of this process—insect protein powder for feed.

[0040] (2) Step (1) gained housefly pupa crude extract is filtered with 300 mesh sieves, and the filtrate is collected;

[0041] (3) The filtrate obtained in step (2) is loaded on an Amicon ultra-rate device (MW50,000, Millipore Company), centrifuged at 3,...

Embodiment 2

[0064] (1) Take a certain amount of housefly pupae, wash and dry with distilled water, add a corresponding volume of 8°C pre-cooled 0.3M acetic acid solution in a volume-to-weight ratio of 1ml:6g, homogenize, extract overnight at 8°C, and place Heat treatment in a water bath at 100°C for 35 minutes, then centrifuge at 6°C and 10,000×g for 35 minutes, and collect the supernatant, which is the crude extract of housefly pupae. After precipitation and drying (110°C), it is crushed into powder, which is one of the by-products of this process—insect protein powder for feed.

[0065] (2) Step (1) gained housefly pupa crude extract is filtered with 500 mesh sieves, and the filtrate is collected;

[0066] (3) The filtrate obtained in step (2) is loaded on an Amicon ultra-rate device (MW30,000, Millipore Company), centrifuged at 5,000×g for 40 minutes at 6°C in a centrifuge with a fixed rotation angle of 35 degrees, and the retained fractions are collected separately and filtered part ...

Embodiment 3

[0070] (1) Weigh a certain amount of housefly pupae, wash and dry with distilled water, add the corresponding volume of 0.2M acetic acid solution pre-cooled at 6°C in a volume-to-weight ratio of 1ml:5g, homogenize, extract overnight at 6°C, and place Heat treatment in a water bath at 95°C for 30 minutes, then centrifuge at 5°C and 9000×g for 30 minutes, and collect the supernatant, which is the crude extract of housefly pupae. After precipitation and drying (105°C), it is crushed into powder, which is one of the by-products of this process—insect protein powder for feed.

[0071] (2) Step (1) gained housefly pupa crude extract is filtered with 400 mesh sieves, and the filtrate is collected;

[0072](3) The filtrate obtained in step (2) is loaded on an Amicon ultra-rate device (MW30,000, Millipore Company), centrifuged at 5°C and 4,000×g for 30 minutes in a centrifuge with a fixed rotation angle of 35 degrees, and the retained parts are collected separately and filtered part o...

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Abstract

The invention relates to a house-fly pupa natural antibiotic peptide product as well as a preparation method and application thereof; house-fly pupas, the raw material of the antibiotic peptide product, are extracted overnight by acid and hot-processed to collect supernatant; and after going through siever filtration and ultrafiltration precessing, the supernatant is treated with spray dring to obtain the antibiotic peptide product. The preparation technique of the antibiotic peptide product is simple and quick, the yield of the antibiotic peptide reaches 1.2 percent and the recovery rate of the antibacterial activity is over 80 percent. The prepared house-fly antibiotic product consists of peptides which have the molecular weight less than 20kDa and are mainly micromolecular peptides with the molecular weight less than 14.4 kDa. The antibiotic peptide product has broad-spectrum antibiotic activity and no hemolytic activity to blood erythrocytes of mice and is characterized by high temperature resistance, acid and alkali resistance and good stability. The byproducts of the technique can serve as feeding insect protein powder. The prepared antibiotic peptide product can be widely applied to the fields such as the antisepsis and storage of foods, fruits and vegetables, feed additives, cosmetic and the like.

Description

technical field [0001] The invention relates to the technical field of biological separation and purification, in particular to a natural antibacterial peptide product of housefly chrysalis and its preparation process, as well as the application of the antibacterial peptide product in the fields of food, fruit and vegetable antiseptic preservation, feed additives and skin care products. Background technique [0002] Since the discovery of antibiotics such as penicillin, a revolutionary leap has taken place in the prevention and treatment of pathogenic bacterial infectious diseases. At the same time, it was also realized that pathogenic microbes would eventually develop resistance to antibiotics such as penicillin, but it was surprising how quickly and broadly resistance developed. With the widespread use and abuse of antibacterial drugs, in less than 60 years, bacteria that were easy to kill began to have drug-resistant strains and multi-drug resistant strains. In order to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/34C07K1/14C07K14/435A61K38/17A61K8/64A23L3/3526A23K1/16A61P31/00A61Q1/00A23J1/00A23K20/147
CPCY02A40/90
Inventor 党向利张文庆汪延生郑小霞王娟王丽芳
Owner SUN YAT SEN UNIV
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