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Method for detecting poppy capsule alkaloid in foodstuff by ultrasonic/microwave synergistic extraction -HPLC

A technology of poppy shell and alkaloid, which is applied in the field of liquid chromatography analysis, can solve the problems of serious detection interference and poor specificity, and achieves the effects of good application prospect, short time consumption and simple operation.

Inactive Publication Date: 2009-03-25
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TLC sensitivity is 1μg, high performance liquid chromatography (HPLC) sensitivity is 1.1ng, but the detection interference of the two methods is serious; immunoassay has high sensitivity, but poor specificity

Method used

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  • Method for detecting poppy capsule alkaloid in foodstuff by ultrasonic/microwave synergistic extraction -HPLC
  • Method for detecting poppy capsule alkaloid in foodstuff by ultrasonic/microwave synergistic extraction -HPLC
  • Method for detecting poppy capsule alkaloid in foodstuff by ultrasonic/microwave synergistic extraction -HPLC

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] 1. Sample processing, the simulated sample is beef soup with poppy shell

[0040] 1-1. Extraction: Take 2mL of the sample to be tested, add 75% ethanol, tartaric acid to adjust the pH to 4, put it into an ultrasonic-microwave synergistic extraction apparatus at 70°C, and extract for 15 minutes;

[0041] 1-2. Filtration: Filter the extracted sample, wash the residue 2-3 times with 75% acidic ethanol with pH 4.0, filter it with absorbent cotton after cooling, wash once with 75% acidic ethanol, and volatilize the ethanol in a water bath to form a syrup , after natural cooling, filter with absorbent cotton, and wash the residue 3-4 times with 10% tartaric acid solution;

[0042] 1-3. Separation: Combine the filtrates in a separatory funnel, degrease twice with 30mL and 20mL petroleum ether, adjust the pH of the water layer to 9.0-9.5 with ammonia water, and then use chloroform:isopropanol at a ratio of 3:1 Extract 30ml, 30ml and 20ml of the mixed solution for 3 times, let ...

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Abstract

The invention provides a method for detecting poppy shell alkaloid in food through ultrasound / microwave synergistic extraction-HPLC. A sample to be detected is subjected to ultrasound / microwave synergistic extraction, filtering, separation and liquid phase chromatographic analysis; morphine, codeine phosphate and papaverine hydrochloride are used as reference substances to obtain characteristic peaks of the morphine, the codeine phosphate and the papaverine hydrochloride; with peak area as an ordinate and the concentration of a standard sample as an abscissa, a standard curve is drawn; and the treated sample is taken out and is subjected to sample introduction with the reference substances of the morphine, the codeine phosphate and the papaverine hydrochloride respectively under the same condition so as to obtain a spectrogram or a standard curve of a detected sample, thereby determining whether the sample contains the poppy shell alkaloid and the content of the poppy shell alkaloid. The ultrasound / microwave synergistic extraction fully utilizes the cavatition of ultrasonic vibration and reduces the bonding force between a target and a sample substrate in order that the poppy composition in the sample is completely extracted. The method has the advantages of sensitivity reaching 0.1ng / L, small experiment interference, simple operation, short time consumption, high reclaiming rate, high precision, and good application prospect in detecting the poppy shell alkaloid in the food.

Description

technical field [0001] The invention relates to an ultrasonic / microwave cooperative extraction-HPLC method for detecting poppy shell alkaloids in food, and belongs to the technical field of liquid chromatography analysis. Background technique [0002] Poppy husk is the dried husk of poppy (Papaver somniferum L), which contains papaverine, morphine, codeine, dibaine, narcotine and other alkaloids. Poppy husk has the effects of astringing the lungs, astringent intestines, and relieving pain. Smoking opium poppy husk and its products can cause the smoker to have strong psychological and physical dependence. The effect of poppy shell on the body is similar to morphine, and the harm of long-term consumption is similar to that of heroin. Therefore, the country lists it as a narcotic drug for control, and prohibits illegal supply, transportation and use. However, there are some illegal operators in the society. In order to attract customers, they add poppy shells to the seasonin...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 王庭欣李小亭冯丽娟赵志磊吴广臣夏立娅刘峥颢庞艳苹
Owner HEBEI UNIVERSITY
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