Conversion method for m-dinitrobenzene, process for producing meta-nitroaniline and m-phenylenediamine

A technology for m-dinitrobenzene and fermentation medium, which is applied in the field of conversion of m-dinitrobenzene, can solve problems such as environmental pollution, high production cost, low yield, etc., achieves low cost, mild conditions, and is conducive to environmental protection Effect

Inactive Publication Date: 2009-04-08
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, the industrial production of m-phenylenediamine basically adopts the reduction method of m-dinitrobenzene iron powder. This method has a long history, simple process and mature technology, but the production cost is high, the yield is low, and it produces a large amount of difficult Treated iron sludge and wastewater containing aromatic amines, causing serious environmental pollution

Method used

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  • Conversion method for m-dinitrobenzene, process for producing meta-nitroaniline and m-phenylenediamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Microbial transformation reaction of Aeromonas hydrophila to m-dinitrobenzene

[0036] The biotransformation of m-dinitrobenzene was carried out by Aeromonas hydrophila, and the bacterial culture medium was: 5 grams of peptone, 1 gram of yeast extract, 23 grams of crude sea salt, 1 liter of water, and the pH of the medium was 7.5. The sterilized medium was inoculated with Aeromonas hydrophila, and cultured in a shake flask at room temperature at a rotational speed of 120 rpm.

[0037] After Aeromonas hydrophila was inserted into the medium and cultivated for 24 hours, m-dinitrobenzene was dissolved in ethanol solvent, 1 g / L m-dinitrobenzene was added to the transformation group, and a blank control group was established at the same time. , that is, adding 1 g / L of m-dinitrobenzene to the blank medium.

[0038] 2. Extraction, separation and identification of transformation products of m-dinitrobenzene transformed by Aeromonas htdrophila

[0039] 1. Extraction of tra...

Embodiment 2

[0049] Vibrio cholerae Vibrio cholerae was used, and others were the same as in Example 1.

Embodiment 3

[0051] Aspergillus sp. was used, and others were the same as in Example 1.

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Abstract

The invention discloses a transformation method of m-dinitrobenzene and a preparation method of m-nitroaniline and m-phenylenediamine. The transformation method adopts microorganisms to carry out the transformation and specifically comprises: the microorganisms are cultured, the m-dinitrobenzene is added in a fermentation culture medium, the microorganisms adopt an organic solvent for extracting the transformed products after the transformation of the m-dinitrobenzene, an extract of the transformed products is obtained by low temperature concentration; and the chromatographic separation is carried out on the extract of the transformed products, while the m-nitroaniline and the m-phenylenediamine are obtained by elution. The transformation method has the advantages of low cost, mild conditions, high efficiency, environmental friendliness, and the like, thereby having high popularization and application value.

Description

technical field [0001] The present invention relates to the conversion method of m-dinitrobenzene and the preparation technology of the conversion product. Background technique [0002] Microbial biotransformation refers to the application of microbial bioreactors (enzymes and multi-enzyme systems, including microbial cells, etc.) to structurally modify and transform precursor compounds to synthesize new organic compounds. Its essence is to use the enzymes produced by the biological system itself to carry out enzymatic reactions to foreign compounds. The types of catalytic reactions include almost all in vitro organic chemical reactions, such as: hydroxylation, oxidation, dehydrogenation, hydrogenation, reduction, hydrolysis, hydration, esters Biotransformation can obtain many substances that are difficult to synthesize by chemical synthesis methods. Microbial biotransformation is mostly carried out at room temperature or in a neutral environment, and has the advantages of ...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12R1/63C12R1/01C12R1/265C12R1/38C12R1/66C12R1/80
Inventor 李厚金蓝文健
Owner SUN YAT SEN UNIV
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