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Method for cultivating disease-resistant maize

A cultivation method and corn technology, applied in the directions of botanical equipment and method, biochemical equipment and method, application, etc., can solve problems such as affecting the growth and yield of corn, and achieve the effect of easy popularization, high speed, and speeding up the breeding process.

Inactive Publication Date: 2009-04-15
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in many corn producing areas, soil salinization seriously affects the growth and yield of corn

Method used

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  • Method for cultivating disease-resistant maize
  • Method for cultivating disease-resistant maize
  • Method for cultivating disease-resistant maize

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Construction and identification of plant expression vectors

[0023] The basic materials in this example are: plasmid pBPC-p5cs-F129A, with Ubiquitin promoter, ampicillin and bar gene selection marker; plasmid pGreen0029-ZmPtil-gfp, with ZmPtil gene (accession number in Genbank is AY708048) and gfp gene.

[0024] The construction method of the plasmid pBPC-p5cs-F129A can be found in the article "Research on the introduction of p5cs gene into tall fescue by gene bombardment" (Li Zhiliang et al., Acta Horticulture, 2005, 32: 653-657); the plasmid pGreen0029- For the construction method of ZmPtil-gfp, please refer to the article "The Location of Corn Protein Kinase ZmSPK1 in Transgenic Arabidopsis" (Xiao Xue et al., North China Agricultural Journal, 2006, 21(3): 1-4).

[0025] A: Construction and identification of the plant sense expression vector

[0026]The plasmid pGreen0029-ZmPtil-gfp was double digested with BamHI and KpnI to obtain a fragment with a size of ab...

Embodiment 2

[0051] Example 2: Transformation by pollen tube channel method

[0052] The maize inbred line 178 was selected as the recipient, and normal-developed plants were selected from the recipient inbred line population during the flowering period for bagging selfing. For specific methods, refer to the paper "Study on Directly Introducing Soybean DNA into Corn Inbred Lines" (Qi Yonghong . Maize Science, 2000, B(1): 34-36.). Introduce within 4-24 hours after pollination. The operation method is: first remove the paper bag from the ear, and use scissors to cut off the silk and bracts about 1-2 cm from the top of the cob, and keep the cut surface of the silk even. And make the filaments in the bracts slightly higher than the bracts. Then use a disposable needle tube to instill 100 μl of plasmid solution with a concentration of 500 ng / μl on the incision, and immediately put the paper bag on again, recording the time of introduction and the name of the plasmid. When the corn ears are mature, ...

Embodiment 3

[0055] Example 3: Preliminary screening of resistance of transformed plants

[0056] A. Detection method:

[0057] The harvested seeds are sown in a seedling tray in an artificial climate chamber, and the culture medium is vermiculite. When T 0 When the generation plant grows three leaves and one core, the core leaves are smeared with a herbicide with a concentration of 0.1%, and the herbicide can be glufosinate.

[0058] B. Test results:

[0059] Figure 5 The following shows the selection of resistant plants after applying the herbicide for one week: Figure 5 -A is non-resistant plants, Figure 5 -B is non-resistant plant leaves, Figure 5 -C is a resistant plant, Figure 5 -D is the resistant plant leaves. by Figure 5 Shown: most of the plant leaves where the herbicide was applied showed a yellow spot, and the periphery of the yellow spot spread to the base of the leaves in a dry shape ( Figure 5 -A, 5-B); the non-transgenic control plants have similar symptoms as above, and...

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Abstract

The invention provides a culturing method of a disease resistance corn. In the method, a plant plus sense expression vector is utilized for inducing the ZmPti1 gene (the logging number is AY708048 in Genbank) of a protein kinase of the corn into the corn and through culturing the disease resistance corn is obtained. The ZmPti1 gene transferred into the corn is a protein kinase gene threateningly induced by salt. Researching the transferring control mechanism of the gene in the corn as well as the application in the approach of an adverse resistance signal is expected to obtain the main effective gene of the corn for resisting the salt; and the gene can be directly applied to a transgene engineering for improving the salt resistance characteristic of crops and quickening the breeding process of the crops. The gene induced in the method does not need to be provided with screening marks and can not cause damages to the environment and human beings; and the method can be adopted for stably and quickly selecting systems. The method does not need expensive devices, is simple to be operated and is easy to be popularized.

Description

Technical field [0001] The invention belongs to the field of methods for cultivating new varieties of transgenic plants by genetic engineering, and particularly relates to a method for cultivating disease-resistant corn by using protein kinase. Background technique [0002] Plants have the ability to quickly perceive and actively adapt to environmental changes, and the strategy of this rapid perception and active adaptation is the transmission of adversity information between and within the plant. This process involves three stages: the plant's perception and recognition of adversity stimuli, the generation and transmission of adversity messengers, and the recognition and transduction of adversity messengers by reaction parts. In this process, the salt-response-related molecules produced by plants can be divided into two categories: one is the effector molecule, which directly participates in the biochemical response process such as metabolic changes, and produces salt tolerance....

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N15/82A01H1/00
Inventor 黄丛林吴忠义张秀海王永勤李春华梁宏霞
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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