Trichoderma reesei liquid submerged fermentation cellulase and enzymatic beating process thereof

Abstract

The invention discloses a submerged fermentation cellulase of trichoderma reesei liquid and an enzymatic pulping process which is a novel process to use the trichoderma reesei as a fermentation strain to produce a cellulase used by self for the enzymatic pulping in a mode of deep liquid fermentation. The technical process is as follows: adopting paper pulp to be processed as a fermentation carbon source; and through the mode of the deep liquid fermentation, preparing the needed cellulase used for the enzymatic pumping after the optimization of a fermentation medium and a fermentation condition. The invention has the advantages of simple process, less equipment investment, low cost and compact technical process; the adoption of the pepper pulp to be processed as the fermentation carbon source can significantly improve the adaptability of an enzyme liquid; and the cellulase has high enzyme activity, less dosage and good effect, and can reduce the energy consumption of the pumping. The submerged fermentation cellulase is suitable for the pulping process of pulping raw materials under the prior structural condition of raw materials for paper making in China.

Description

technical field [0001] The invention relates to a cellulase enzymatic beating process, in particular to a Trichoderma reesei liquid submerged fermentation cellulase and an enzymatic beating process thereof. Background technique [0002] Pulping is an important link in the pulping and papermaking process, which has an effect on the physical properties of the paper web. However, beating is an energy-consuming process. In order to obtain the required pulp properties, beating or refining will consume a large amount of energy, accounting for about 18% of the total energy required from raw materials to paper. Therefore, any pulp treatment method that significantly reduces energy consumption for beating or refining will have a beneficial effect on the overall energy input. [0003] PRATIMA BAJPAI published in "TAPPI Journal", Volume 5, Issue 11, Page 25-32, "Use of enzymes for reduction in refining energy-laboratory studies" in 2006, showed that: Biorefine L cellulase from Biocon...

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Problems solved by technology

Due to the relatively high price of commercial cellulase, the cost of using the factory is very high. In addition, there is a shortage of domestic papermaking raw materials. The fibers of most fast-growing woods such as masson pine, larch and Simao pine are thick and long. Salt pulp is difficult to beat, which makes the market competitiveness of commercial pulp weak and economic benefits poor. Therefore, the paper industry urgently needs to develop an enzymatic beating process based on the specific conditions of the production plant. Achieve the effect of reducing production cost and beating energy consumption

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