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Pyrethroid insecticide hydrolytic enzyme genes

A technology of pyrethroid insecticides and hydrolytic enzymes, applied in the fields of hydrolytic enzymes, genetic engineering, plant genetic improvement, etc., can solve unrealistic problems, achieve the effect of improving product quality and increasing added value

Inactive Publication Date: 2009-05-13
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But as far as the current situation is concerned, human beings still face hunger and poverty. It is unrealistic to fully implement organic agriculture and green agricultural production, and to ban the use of pesticides.

Method used

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  • Pyrethroid insecticide hydrolytic enzyme genes
  • Pyrethroid insecticide hydrolytic enzyme genes
  • Pyrethroid insecticide hydrolytic enzyme genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] 1. Cloning of pyrethroid hydrolase gene

[0022] 1.1 Extraction of total DNA from Sphingomonas sp.JQ1-11 (GenBank registration number DQ132883)

[0023] The high-purity and high-molecular-weight JQ1-11 total DNA was extracted by CTAB method, dissolved in TE (pH8.0), and stored at -20°C. For specific methods, refer to "Refined Molecular Biology" edited by F. Osper et al. Experiment Guide".

[0024] 1.2 PUC118 (BamHI) was purchased from Bao Biological Engineering (Dalian) Co., Ltd.

[0025] 1.3 Digestion of total DNA The total DNA of Sphingomonas sp.JQ1-11 was partially digested with Sau3AI.

[0026] 1.4 Recovery of DNA

[0027] The total DNA after enzyme digestion was purified by electrophoresis (TAE buffer) and recovered using axygen biosciences (China) recovery kit. The recovered DNA was dissolved in 10 mmol / L Tris Cl (PH8.0) and placed in- Store at 20°C.

[0028] 1.5 Enzyme Ligation Establish the following reaction system:

[0029] PUC118 (BamHI) 0.1 μg...

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Abstract

The invention provides a hydrolase gene of pyrethroid pesticide, which belongs to the field of application environment microbiology. The invention provides the hydrolase gene of the pyrethroid pesticide and pyrethoid hydrolase protein derived by the hydrolase gene. The whole length of the gene is 843bp, the content of G and C is 66.55 percent and 280 amino acids are coded. An engineering strain constructed by the gene can express pyethroid hydrolase with high efficiency, and the produced enzyme preparation can be used for removing pyrethroid residual of soil, vegetables, tea leaves, fruits, Chinese herbal medicines, tobacco and the like, and degrading pyrethroid pesticide residual in deep processing of agricultural products, thereby improving product quality and increasing added value. The hydrolase gene can be further used for constructing transgene crops to completely solve the problem of pesticide residual in plant, and producing nuisanceless green agricultural products; meanwhile, the hydrolase gene can be used for restoring water body and other environments polluted by the pyrethroid.

Description

technical field [0001] The invention relates to a pyrethroid insecticide hydrolase gene (also known as a degradation gene), which belongs to the field of applied environmental microorganisms and is used for removing pyrethroid insecticide residues in crops and soil to produce pollution-free green agricultural products; at the same time, it can be used It can be used to restore the environment such as water bodies polluted by permethrin. Background technique [0002] Although the use of pesticides has improved agricultural production efficiency and crop yields, it has also brought about serious environmental problems, resulting in ecological imbalances, declining quality of agricultural products, and directly affecting people's health. Pyrethroid insecticide is a high-efficiency broad-spectrum insecticide discovered by M.Eliiott et al. in 1974. At present, the annual sales of pyrethroid insecticides are 1.3-1.4 billion US dollars, accounting for the world's pesticide sales 2...

Claims

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Application Information

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IPC IPC(8): C12N15/55C12N9/16C12N15/63C12N1/21A62D3/02C12R1/19A62D101/04
Inventor 李顺鹏何健王保战郭鹏李恋杭宝剑
Owner NANJING AGRICULTURAL UNIVERSITY
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