Method for hemachrome or hematocrit detection by electro-chemistry method, and detection test piece thereof

A technology of heme and hematocrit, which is applied in the field of electrochemical detection of heme concentration, can solve the problems of how to improve the dissolution characteristics of electron transfer substances, etc.

Active Publication Date: 2012-12-19
GENERAL LIFE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

U.S. Patent No. 7,138,041 discloses a sensory test strip that uses electrochemical detection. However, this patent does not disclose how to improve the dissolution characteristics of electron transfer substances and directly use it for heme detection.

Method used

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  • Method for hemachrome or hematocrit detection by electro-chemistry method, and detection test piece thereof
  • Method for hemachrome or hematocrit detection by electro-chemistry method, and detection test piece thereof
  • Method for hemachrome or hematocrit detection by electro-chemistry method, and detection test piece thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0070] 0.102 g (0.5 mmol) of tetrathiafulvalene (tetrathiafulvalene, TTF), 2.555 g (1.75 mmol) of hydroxypropylmethyl-β-cyclodextrin, and 10 ml of deionized water were added to avoid In a light dark container, stir overnight at room temperature. Then, it was centrifuged at 6000 rpm for 10 minutes, and the supernatant was taken to obtain an electron carrier solution including 50 mM TTF and 175 mM hydroxypropylmethyl-β-cyclodextrin.

preparation example 2

[0072] Using a polyethylene terephthalate bottom plate substrate, the conductive silver glue was printed on the surface of the substrate by screen printing, and dried at 60° C. for 5 minutes to form a conductive silver glue layer. Next, apply carbon glue by screen printing and dry it at 60°C for 5 minutes to form a working electrode; then brush silver / silver chloride glue on it by screen printing and dry it at 60°C for 5 minutes to form a working electrode. Reference electrode. Then, apply insulating glue, dry at 105° C. for 5 minutes, and then attach a spacer sheet with specimen diversion grooves and exhaust grooves. Apply 5 microliters of the reaction reagent, which contains 10 mM electron carrier solution (preparation example 1), 2% polyvinylpyrrolidone, and 50 mM potassium phosphate buffer solution (pH 8.0) to the sample diversion tank. , 10-100mM potassium chloride, 1% Triton X-100, and 0.5% methyl carbonate fiber, after drying at 50°C for 15 minutes, paste the second sp...

preparation example 3

[0074] Repeat the preparation example 2, and change the electron transfer substance solution in the reaction reagent to 5 mM, which is the test piece sample 2.

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Abstract

The invention relates to a method for detecting hemachrome or hematocrit by making use of electrochemistry and a detection test piece thereof, wherein, the detection method comprises: (1) a sample and reagent are contacted, and the reagent comprises buffer solution, interface active agent and a tetrathiafulvalene electric transmitter modified by cyclodextrine; (2) under the condition of rated electric potential, the electric current generated through the reaction between the hemachrome and the electric transmitter is measured; and (3) the hemachrome concentration in the sample is calculated according to the measured electric current. By making use of the tetrathiafulvalene formed through the modification of the cyclodextrine as the electric transmitter which directly acts with the hemachrome in the sample under condition of rated electric potential, the method can rapidly detect the hemachrome concentration in the full-blood sample or is further used for calculating the hematocrit of the blood sample by making use of the inverse relation between electric quantity value of electric current or electric current and time integral and the hemachrome concentration.

Description

technical field [0001] The present invention relates to a method for detecting the hemoglobin concentration by electrochemical method and a test piece, in particular to a method for detecting the hemoglobin concentration of whole blood samples by electrochemical method and the test piece. Background technique [0002] In blood testing, besides blood sugar and cholesterol, hemoglobin is also an important biochemical testing target. The methods currently used to detect heme include the copper sulfate specific gravity method for measuring the specific gravity of whole blood; the chemical method of using the partial structure Heme in heme to have peroxidase activity to carry out redox reactions; using the antibody of HbA1 to detect feces The immune method of hemoglobin; the gas detection method using the combination of carbon monoxide and heme; the use of potassium ferricyanide (K 3 Fe(CN) 6 ) converts heme into denatured heme, and then forms cyanodenatured heme with sodium cy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/72G01N27/26
Inventor 苏建雄吴太光
Owner GENERAL LIFE BIOTECH
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