Pyrethroid pesticide artificial antigen, antibody and preparation thereof

A pyrethroid, artificial antigen technology, applied in chemical instruments and methods, animal/human proteins, serum albumin and other directions, can solve the problems of high preparation cost, many synthesis steps, low sensitivity of antibody analysis, etc., and achieve high sensitivity , The effect of low preparation cost

Inactive Publication Date: 2009-05-20
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In the preparation methods of artificial antigens of pyrethroid pesticides that have been reported abroad, the synthesis conditions of the hapten coupled with the carrier protein are very harsh, and there are many synthesis step

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pyrethroid pesticide artificial antigen, antibody and preparation thereof
  • Pyrethroid pesticide artificial antigen, antibody and preparation thereof
  • Pyrethroid pesticide artificial antigen, antibody and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1

[0031] 1) The hapten synthesis method is as follows:

[0032] Dissolve 1.63g (5mmol) of α-cyano-m-phenoxybenzyl alcohol in 5mL of dichloromethane to prepare a solution with a concentration of 1mol / L. After cooling to the reaction temperature, add it to the reaction flask; add 0.62g (6mmol ) Succinic anhydride was dissolved in 2 mL of dichloromethane to prepare a solution with a concentration of 3 mol / L. After cooling to the reaction temperature, it was added dropwise to the reaction flask. After the addition, the reaction was stirred and reacted for 12 hours. The reaction temperature was 14°C. The molar ratio of α-cyano-m-phenoxybenzyl alcohol and succinic anhydride is 1:1.2. Pyrethroid hapten is generated after the reaction is over (Where R=CN).

[0033] Electrospray mass spectrometry and nuclear magnetic resonance were used to identify the structure of the generated type I pyrethroid universal hapten. The identification result was: ESI-MS: 324(M-H) - (100%); 1...

Example Embodiment

[0045] Example 2

[0046] 1) The hapten is synthesized according to the method in the invention patent "Type I pyrethroid pesticide general hapten and its synthesis method" (application number:), the synthesis method is as follows:

[0047] Dissolve 1.18g (5mmol) of m-phenoxybenzyl alcohol in 5mL of dichloromethane, make a 1mol / L solution, cool to 18°C, add it to the reaction flask after cooling to 18°C; add 1.02g of succinic anhydride ( 10mmol) was dissolved in 10mL of dichloromethane to make a 1mol / L solution. After cooling to 18℃, it was added dropwise to the reaction flask. After the addition, the reaction was stirred and reacted for 15 hours. The reaction temperature was 18℃. The reaction material was m-phenoxy. The molar ratio of benzyl alcohol and succinic anhydride is 1:2. After the reaction, most of the organic solvents were removed by rotary evaporation, and then purified by silica gel column chromatography to obtain 1.47 g (4.9 mmol) of a white solid product, with a yie...

Example Embodiment

[0060] Example 3

[0061] 1) Pyrethroid hapten The synthesis and identification of (where R=CN) is the same as step 1) of Example 1.

[0062] 2) Coupling the synthesized hapten compound with protein to prepare artificial antigen, including immune antigen and coating antigen, the method is as follows:

[0063] The hapten synthesized above 0.11g (0.32mmol) (Where R=CN), equimolar amount of N,N-dicyclohexylcarbodiimide and equimolar amount of N-hydroxysuccinimide are dissolved in 0.6mL of N,N-dimethylformamide, The reaction was stirred at 20°C for 4 hours. After the reaction, the reaction mixture was placed at 4°C overnight (more than 10 hours), then centrifuged, 0.48 mL of the supernatant was taken, divided into two equal parts, and slowly added to 3mL of 15mg / mL hemocyanin in a carbonate buffer solution, react for 14 hours under stirring. After the reaction is complete, put it into a dialysis bag and dialyze 8 times with a regular phosphate buffer of pH 7.4 as a dialysate The dia...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an artificial antigen and an antibody of a pyrethroid pesticide and a preparation method thereof. The artificial antigen is a chemical compound of an artificial antigen of the pyrethroid pesticide, and contains a majority of commonness configuration of the pyrethroid pesticide; the artificial antibody is a polyclonal immunoglobulin G which is prepared by the artificial antigen compound through the immune animal and can react with more than two pyrethroid pesticides for specific immune response. The method has the strengths of easy obtainment of a half antigen of the artificial antigen of the pyrethroid pesticide and environmental friendliness which lower the preparation cost of the artificial antigen; and in the prepared artificial antigen, the common ester bond configuration of the pyrethroid pesticide is retained simultaneously which facilitates the preparation of the high-quality antibody; the antibody has the advantages of high sensitivity and recognizing various pyrethroid pesticides. The antibody can be applied to develop the rapid analytical techniques, methods, and products of multi-residue immunity of the pyrethroid pesticide in the agricultural products and foods.

Description

technical field [0001] The invention relates to a pyrethroid pesticide artificial antigen, antibody and preparation method prepared by selecting a compound having -COOH and containing most of the common structures of pyrethroid pesticides as a hapten. Background technique [0002] The problem of pesticide residues has become a hot issue of general concern in today's society. Pyrethroid pesticides are one of the three major categories of insecticides, which have the characteristics of broad insecticidal spectrum, moderate toxicity, and accumulation. With the prohibition or restriction of highly toxic organophosphorus and carbamate pesticides, the use of pyrethroid pesticides has become more and more widespread, but the problem of such pesticide residues has also become increasingly apparent. The problem of excessive pyrethroid pesticide residues is the most common problem in the export trade of agricultural products such as tea in my country. Therefore, monitoring pyrethroi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K14/765C07K14/795C07K14/77C07K16/44C07K16/06
Inventor 李培武张奇张文谢立华丁小霞姜俊陈小媚
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap