Clean production technology for separation and extraction of catalase

A catalase and clean production technology, which is applied in the field of separation and extraction of biologically active substances, can solve problems such as environmental pollution, a large amount of waste residue and waste water, and achieve the effect of eradicating environmental pollution problems

Inactive Publication Date: 2009-06-03
江苏省江大绿康生物工程技术研究有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] No matter which raw material is used, the current popular catalase processing and production technology has a common defect in the process, that is, a large amount of waste residue and waste water will be generated during the production process, causing very serious pollution to the environment
[0006] It can overcome this defect, make the processing and production of catalase in an environment-friendly way, realize zero discharge of waste residue and wastewater in the processing and production process, and completely eradicate the environmental pollution problem in the process of catalase processing and production in China. Haven't seen any reports outside

Method used

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  • Clean production technology for separation and extraction of catalase
  • Clean production technology for separation and extraction of catalase
  • Clean production technology for separation and extraction of catalase

Examples

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Effect test

example 1

[0014] Take 10kg of pig liver, chop it up, add 40L of water to homogenate it, and keep it warm at 30°C for 12 hours to make it autolyze. After reaching the specified time, adjust the pH value to 6.5, and then vacuum filter to obtain 40 L of filtrate with peroxidase activity of 20,000 U / ml and 9.5 kg of filter residue. The filtrate was concentrated by ultrafiltration with a hollow fiber ultrafiltration membrane with a cut-off molecular weight of 40 kDa to obtain 15 L of ultrafiltration concentrate with a peroxidase activity of 50,000 U / ml and 25 L of ultrafiltration permeate. The ultrafiltration permeate was concentrated with a roll-type reverse osmosis membrane to obtain 1.2 L of reverse osmosis concentrate and 23.7 L of reverse osmosis permeate. The reverse osmosis concentrate was combined with the filter residue, and dried by a drum to obtain 2.3 kg of solid. Add water to the reverse osmosis permeate to 40L, repeat the above process as process water, and get roughly the sam...

example 2

[0016] Take 5kg of chicken liver, chop it up, add 20L of water to homogenate it, and keep it warm at 35°C for 8 hours to make it autolyze. After reaching the specified time, adjust the pH value to 6.5, and then use a refrigerated centrifuge to centrifuge at 10°C and 9000 rpm to obtain 20 L of supernatant with a peroxidase activity of 28,000 U / ml and 5 kg of residue. The supernatant was concentrated by ultrafiltration with a hollow fiber ultrafiltration membrane with a cut-off molecular weight of 20 kDa to obtain 11 L of ultrafiltration concentrate with a peroxidase activity of 50,000 U / ml and 9 L of ultrafiltration permeate. The ultrafiltration permeate was concentrated with a roll-type nanofiltration membrane (molecular weight cut-off: 150) to obtain 0.4 L of nanofiltration concentrate and 8.5 L of nanofiltration permeate. The nanofiltration concentrate was combined with the residue, and dried in vacuo to obtain 1.3 kg of solid. Add water to the nanofiltration permeate to 20...

example 3

[0018] Get the fermented liquid 500kg that produces catalase Aspergillus niger, adjust pH value to 6.5, centrifuge at 10 ℃ with refrigerated centrifuge then at 9000rpm, obtain peroxidase activity and be the supernatant 450L of 5000U / ml, Residue 50kg. The supernatant was concentrated by ultrafiltration with a roll-type ultrafiltration membrane with a cut-off molecular weight of 40 kDa to obtain 42 L of ultrafiltration concentrate with a peroxidase activity of 50,000 U / ml and 408 L of ultrafiltration permeate. The ultrafiltration permeate was concentrated and treated with a roll-type nanofiltration membrane (molecular weight cut-off: 300) to obtain 20 L of nanofiltration concentrate and 386 L of nanofiltration permeate. The nanofiltration concentrate was combined with the residue, and air-dried to obtain 15 kg of solid. Add water to the nanofiltration permeate to 500L, repeat the above process as process water, and obtain roughly the same result.

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Abstract

The invention relates to the technical field of separation and extraction of bioactive substances, in particular relates to a clean production technique for the separation and the extraction of catalase, and provides an environment-friendly technique for the separation and the extraction of catalase. The technique can realize zero-discharge of waste water and waste residue during the process of processing and producing catalase and radically solve the environment pollution problem in the prior technique of processing and producing catalase without influencing the yield and quality of catalase. The technique is suitable for the processing and the production of catalase by using the raw material of the liver of various animals such as pig, cattle, ship, chicken, duck, goose, fish, etc after autolysis treatment, and is also suitable for the processing and the production of catalase by using the catalase-containing fermentation liquid obtained through the fermentation and the cultivation of microbe. According to the invention, taking pig liver as an example, 2 kg of catalase liquid (50,000 U/ml) can be prepared from 1 kg of pig liver, the water consumption is reduced to lower than 2.2 kg, and 230 g of the solid substances with protein content being higher than 60% can be obtained at the same time.

Description

technical field [0001] The invention relates to a clean production process for the separation and extraction of catalase, belonging to the technical field of separation and extraction of biologically active substances. Background technique [0002] Catalase is an oxygen-removing enzyme whose function is to efficiently decompose hydrogen peroxide into water and oxygen, and has a wide range of uses in textile, food and other industries. In the textile industry, catalase is applied to the biological deoxygenation and purification process of textiles after oxygen bleaching, which can greatly save water and energy, and remove the dye decomposition that may occur in subsequent dyeing of reactive dyes in fabrics and water baths after oxygen bleaching Or the residual hydrogen peroxide of dyeing defects, shorten the process time, improve the dyeing quality, and reduce the discharge of printing and dyeing wastewater. The enzyme is also suitable for hotels, hospitals, laundry rooms an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08
Inventor 汤鲁宏吴保承戴永琪杨春霞邓超陈伟徐玲燕
Owner 江苏省江大绿康生物工程技术研究有限公司
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