Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ammonia ion diagnosis/measuring reagent kit and ammonia ion concentration determination method

A kit and ion technology, which is applied in the field of ammonia diagnosis/determination kits and determination of ammonia concentration, and can solve problems such as undiscovered blood ammonia determination methods

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching Chinese patents, only found 87105593.7 patent application which discloses a quick-frozen cup for measuring blood ammonia, but did not find an ideal method for measuring blood ammonia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0045] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0046] Stabilizer 500mmol / L

[0047] Coenzyme 3mmol / L

[0048] Glutamine Synthetase 10000U / L

[0049] Glycerol kinase 12000U / L

[0050] Glycerol dehydrogenase 12000U / L

[0051] Glutamic acid 8mmol / L

[0052] Adenosine triphosphate 3mmol / L

[0053] Glycerol-3-phosphate 5mmol / L

[0054] Magnesium chloride 2mmol / L

[0055] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0056] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the ammonia (ion) sample to the reagent is 1 / 25. The reaction direction is posit...

Embodiment 2

[0059]The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0060] Reagent 1

[0061] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0062] Stabilizer 50mmol / L

[0063] Coenzyme 3mmol / L

[0064] Glutamic acid 8mmol / L

[0065] Adenosine triphosphate 3mmol / L

[0066] Glycerol-3-phosphate 5mmol / L

[0067] Magnesium chloride 2mmol / L

[0068] Reagent 2

[0069] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 50mmol / L

[0071] Glutamine Synthetase 10000U / L

[0072] Glycerol kinase 12000U / L

[0073] Glycerol dehydrogenase 12000U / L

[0074] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0075] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, ...

Embodiment 3

[0078] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0079] Reagent 1

[0080] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 50mmol / L

[0082] Coenzyme 3mmol / L

[0083] Glutamic acid 8mmol / L

[0084] Adenosine triphosphate 3mmol / L

[0085] Glycerol-3-phosphate 5mmol / L

[0086] Magnesium chloride 2mmol / L

[0087] Reagent 2

[0088] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0089] Stabilizer 500mmol / L

[0090] Glycerol kinase 12000U / L

[0091] Glycerol dehydrogenase 12000U / L

[0092] Reagent 3

[0093] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0094] Stabilizer 500mmol / L

[0095] Glutamine Synthetase 10000U / L

[0096] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0097] When measuring ammonia (ion) concentration, set...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a kit for diagnosing / measuring ammonia (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of ammonia (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, coenzyme, glutamic acid, adenyl pyrophosphate, glyceraldehydes-3-phosphoric acid, magnesium chloride, glutamoyl synthetase, glycerokinase, glycerol dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of ammonia (ions).

Description

technical field [0001] The invention relates to an ammonia (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring ammonia (ion) concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] Ammonia determination methods include micro-diffusion method, ion exchange method, enzymatic method and ammonia electrode method. The most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode. [0003] The diffusion method is that after the sample is alkalized, NH is released 3 , Titrate the released ammonia with acid, or use the Nessler reaction to form brown-yellow mercuric ammonium iodide for colorimetry. These methods need to be alkalized, and the endogenous ammonia formation will affect the accuracy and precision, so they are rarely used at present; the ion exchange method is more accurate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com