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Magnesium (ion) diagnosis/measuring reagent kit and magnesium (ion) concentration determination method

A kit and ion technology, applied in the field of magnesium diagnosis/determination kits and determination of magnesium concentration, can solve problems such as calcium interference and interference

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is also calcium interference (within the physiological range) Ca 2+ Maximum interference 10% etc. insufficient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The magnesium (ion) diagnosis / determination reagent of the present embodiment is a single reagent, comprising:

[0049] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0050] Stabilizer 500mmol / L

[0051] Reduced coenzyme 0.25mmol / L

[0052] Hexokinase 6000U / L

[0053] Pyruvate kinase 8000U / L

[0054] Alanine dehydrogenase 10000U / L

[0055] Glucose 20mmol / L

[0056] Adenosine triphosphate 3mmol / L

[0057] Phosphoenolpyruvate 3mmol / L

[0058] Ammonium chloride 3mmol / L

[0059] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0060] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured magnesium (ion) sample to reagent is 1 / 25, the reaction direction is neg...

Embodiment 2

[0063] The magnesium (ion) diagnosis / determination reagent of the present embodiment is a double reagent, comprising:

[0064] Reagent 1

[0065] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 50mmol / L

[0067] Reduced coenzyme 0.25mmol / L

[0068] Glucose 20mmol / L

[0069] Adenosine triphosphate 3mmol / L

[0070] Phosphoenolpyruvate 3mmol / L

[0071] Ammonium chloride 3mmol / L

[0072] Reagent 2

[0073] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0074] Stabilizer 500mmol / L

[0075] Hexokinase 6000U / L

[0076] Pyruvate kinase 8000U / L

[0077] Alanine dehydrogenase 10000U / L

[0078] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0079] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, measured...

Embodiment 3

[0082] The magnesium (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprises:

[0083] Reagent 1

[0084] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0085] Stabilizer 50mmol / L

[0086] Reduced coenzyme 0.25mmol / L

[0087] Glucose 20mmol / L

[0088] Adenosine triphosphate 3mmol / L

[0089] Phosphoenolpyruvate 3mmol / L

[0090] Ammonium chloride 3mmol / L

[0091] Reagent 2

[0092] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0093] Stabilizer 500mmol / L

[0094] Pyruvate kinase 8000U / L

[0095] Alanine dehydrogenase 10000U / L

[0096] Reagent 3

[0097] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0098] Stabilizer 500mmol / L

[0099] Hexokinase 6000U / L

[0100] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0101] When measuring magnesium (ion) concentration, set on th...

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Abstract

The invention relates to a kit for diagnosing / measuring magnesium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of magnesium (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glucose, adenyl pyrophosphate, enolphosphopyruvate, ammonium chloride, hexokinase, pyruvate kinase, lactamine dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of magnesium (ions).

Description

technical field [0001] The invention relates to a magnesium (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring magnesium (ion) concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] There are many methods for the determination of magnesium, which can be summarized as colorimetry, fluorescence method, ion chromatography, ion selective electrode method (ISE), enzymatic method, atomic absorption spectrophotometry (AAS), isotope dilution mass spectrometry (ID-MS) Wait. [0003] The definitive method for the determination of magnesium is isotope dilution mass spectrometry, and the reference method is atomic absorption spectrophotometry. Although the results of these methods are accurate, the equipment is complex and expensive, so they are not suitable for routine laboratory and automatic analysis. [0004] Colorimetry is the most widel...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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