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Isolated culture method and use of Streptomycete from sea

A technique for isolating and cultivating Streptomyces, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems that material research has just started

Inactive Publication Date: 2009-08-05
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to incomplete statistics, the current research on the active substances of marine actinomycetes has attracted people's attention. A variety of marine actinomycetes producing new active substances have been isolated from the marine environment, and the fermentation conditions, biological The activity and the separation and identification of active substances have been studied, and gratifying results have been obtained, but the existing research mainly focuses on the development of medicine, and the research on the antibacterial substances of marine actinomycetes on plant pathogenic fungi has just started. Finding marine actinomycetes with new antibacterial mechanisms in biological control of plant diseases is of great significance for the development of new efficient and environmentally friendly pesticides

Method used

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  • Isolated culture method and use of Streptomycete from sea
  • Isolated culture method and use of Streptomycete from sea
  • Isolated culture method and use of Streptomycete from sea

Examples

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Embodiment 1

[0132] Example 1. A method for isolation and culture of Streptomyces sp.BM-2 from the ocean, the steps are as follows:

[0133] (1) Enrichment culture: collect sea mud from the sea area of ​​Lianyungang, put 10 g of sea mud into a triangular flask filled with 50 mL of sterile seawater, shake and shake well, take 5 mL of suspension and add it to 50 mL of enrichment culture medium, 190r / min, cultured on a shaker at 25°C for 5 days;

[0134] (2) Separation and purification of bacteria: use a pipette gun to take 1.0 mL of the culture solution enriched and cultivated for 5 days and add it to a test tube containing 9.0 mL of sterile seawater to prepare 10 -1 The sample diluent; and then serially diluted by gradient dilution method, respectively prepared into 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 sample diluent; then take 10 -5 、10 -6 and 10 -7 Put 0.2ml of the sample dilution solution on the seawater Gaoshi No. 1 medium plate, spread it evenly with a coating rod, cultu...

Embodiment 2

[0138] Example 2. In the method for isolating and cultivating Streptomyces sp.BM-2 from the sea described in Example 1, what is the result of the obtained Streptomyces sp.BM-2 strain? The fermentation conditions are: the optimum medium is: 14g starch, 16g lactose, 16g soybean powder, 3g dipotassium hydrogen phosphate, 4g calcium carbonate, 0.4g potassium chloride, 30g sodium chloride, 1000ml water, pH 7.5; The method is as follows: inoculation amount 7%, bottling amount 250ml Erlenmeyer flask 80ml / bottle, shaker speed 190r / min, optimum temperature 28°C, culture time 5d.

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Abstract

The invention relates to a separation culture method of BM-2 (Streptomyces sp.BM-2) from sea, which is characterized in that the method comprises the following steps of accumulation culture, thalli separation and purification, strain screening, strain identification, and the like. According to the test results of morphology, physiology and biochemistry, and the analysis of the homology of 16SrDNA, separated and cultured stains are BM-2(Streptomyces sp. BM-2). The BM-2(Streptomyces sp. BM-2) has the function of inhibiting various plant pathogenic fungi, colon bacillus, bacillus subtilis and aeromonas hydrophila.

Description

technical field [0001] The invention relates to a method for cultivating strains, in particular to a method for isolating and cultivating Streptomyces BM-2 from the sea; the invention also relates to the application of Streptomyces BM-2. Background technique [0002] Secondary metabolites of many actinomycetes have medicinal and plant protection applications, and have been widely used as antibacterial, antifungal and antitumor drugs. Among the tens of thousands of biologically active substances derived from microorganisms that have been discovered, about 70 % is synthesized by actinomycetes. At present, the vast majority of actinomycetes isolated come from soil, and the antibiotics produced by terrestrial actinomycetes account for more than two-thirds of the antibiotics from natural sources. However, with the increasing resistance of pathogenic microorganisms to antibiotics, it is necessary to find new mechanisms of action. Antibiotics are imminent. In the past 20 years, t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/04A01P1/00A01P3/00C12R1/465
Inventor 马桂珍暴增海吴少杰张晓君王伟霞李福后
Owner HUAIHAI INST OF TECH
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