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Cultivation fermentation method of Bacillus coagulans antimycotics active substance

A technology of bacillus coagulans and fermentation method, which is applied in the field of cultivation and fermentation of antifungal active substances of bacillus coagulans, can solve problems such as patents that have not been reported, and achieve the effects of improving economic benefits, being suitable for mass production, and low production cost

Inactive Publication Date: 2012-04-11
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report in the literature or patents on the fermentation conditions and the application of fermented products of Bacillus coagulans to produce antifungal active substances

Method used

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  • Cultivation fermentation method of Bacillus coagulans antimycotics active substance

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Experimental program
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Effect test

Embodiment 1

[0023] A method for cultivating and fermenting an antifungal active substance of bacillus coagulans, the steps of which are:

[0024] (1) Seed culture on slant plane: Take a ring of bacteria slime and inoculate it by streaking on the nutrient gravy agar medium, and cultivate it at 40°C for 20 hours;

[0025] (2) Seed shake flask culture: Take a ring of slant and insert it into a 250ml Erlenmeyer flask equipped with 25ml MRS medium, and cultivate it at 40°C and 140r / min for 20h to become a first-class seed solution;

[0026] (3) Shake flask fermentation culture: with 5% inoculum size, the primary seed solution is inserted into a 500ml Erlenmeyer flask equipped with 120ml fermentation medium, cultivated at 40°C for 18h at 180r / min to become the secondary seed solution;

[0027] (4) 5L fermenter cultivation: the secondary seed liquid is inserted in the 5L automatic fermenter that 3.5L fermentation medium is housed with 5% inoculation amount, and setting liquid loading is 70%, and...

Embodiment 2

[0033] (1) Seed culture on slant: take a ring of bacteria slime and inoculate it on the nutrient gravy agar medium, and cultivate it at 40°C for 20 hours;

[0034] (2) Seed shake flask culture: Take a ring of slant and insert it into a 250ml Erlenmeyer flask equipped with 25ml MRS medium, and cultivate it at 40°C and 140r / min for 20h to become a first-class seed solution;

[0035] (3) Shake flask fermentation culture: with 5% inoculum size, the primary seed solution is inserted into a 500ml Erlenmeyer flask equipped with 120ml fermentation medium, cultivated at 40°C for 18h at 180r / min to become the secondary seed solution;

[0036] (4) 30L fermenter cultivation: the secondary seed liquid is inserted in the 30L automatic fermenter that 21L fermentation medium is housed with 3.6% inoculum (filling capacity is 70%), and initial stirring speed is 100r / min, The ventilation rate is 1:0.3, and the constant dissolved oxygen is 100%. When the dissolved oxygen drops below 30%, increas...

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Abstract

The invention relates to a culture fermentation method for a Bacillus coagulans antifungal active substance. The method can obtain a bacterium with high biomass and antifungal activity by taking the Bacillus coagulans (CGMCC 1.949) as material, and a fermentation process of the method can be used for preparing fungicides for preventing and controlling plant diseases. The method has the following three advantages: 1, the Bacillus coagulans is a type of probiotics beneficial to human bodies, and the biggest difference between biological fungicides prepared from metabolite of the Bacillus coagulans and the prior fungicides lies in natural property and environment-friendly property thereof; 2, a set of fermentation process established according to the bacterium substitutes soybean cake powderand corn starch for beef extract, peptone and the like greatly reduces production cost; and 3, after the bacterium is fermented, fermentation-product supernatant can be used for preparing the biological fungicides, and thalli obtained through centrifugation can continue to be utilized for producing probiotic products, so that economic benefit is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for cultivating and fermenting antifungal active substances of Bacillus coagulans (CGMCC1.949). Background technique [0002] Worldwide, agricultural production causes huge losses every year due to pests and weeds, of which 80% are caused by fungi. For a long time, people have mainly used chemical pesticides to control crop diseases, insect pests and weeds to ensure stable and high yield of crops. However, the extensive use of chemical pesticides not only pollutes soil, water and the atmosphere; moreover, the residues of chemical pesticides in agricultural by-products will directly endanger human health and survival. At the same time, due to the long-term, large-scale and repeated use of chemical pesticides, more and more plant pathogenic fungi have developed resistance to chemically synthesized antifungal agents and antibiotics. Therefore, it is necessary to de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/04A01N63/02A01P3/00C12R1/07
Inventor 戚薇王海宽张和平杜连祥王晨颜虎石景孙天松孙志宏
Owner TIANJIN UNIV OF SCI & TECH