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Lotus Leaf extract, preparation method and application thereof

A lotus leaf extract and lotus leaf technology, applied in the field of preparation of the lotus leaf extract, can solve the problems of no acute liver injury protection and therapeutic effect, and achieve the effect of increasing the SOD activity value

Inactive Publication Date: 2011-09-07
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The pharmacological activity of lotus leaf is mainly studied by its hydroalcoholic extract, and there are no relevant reports on the protective and therapeutic effects of its ethyl acetate and n-butanol extracts on carbon tetrachloride-induced acute liver injury

Method used

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  • Lotus Leaf extract, preparation method and application thereof
  • Lotus Leaf extract, preparation method and application thereof
  • Lotus Leaf extract, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] This embodiment is the preparation method of lotus leaf extract——ethyl acetate extract and n-butanol extract. The volume ratio of acetone and water in the mixed solution is: acetone / water=7 / 3, 7 days for the first time, 3 days for the last two times, suction filtration, combined filtrates and reclaiming acetone under reduced pressure to obtain lotus leaf aqueous solution, the second time First extract with ethyl acetate to obtain ethyl acetate extract; extract the remaining solution with n-butanol to obtain n-butanol extract.

Embodiment 2

[0023] This example is an experiment on the hepatoprotective effect of lotus leaf ethyl acetate extract and n-butanol extract.

[0024] METHODS: In Vivo-Carbon Tetrachloride Induced Acute Liver Injury Model in Mice

[0025] Principle: CCl 4 It is the earliest and most widely used inducer of liver injury and liver fibrosis, and it is the most commonly used drug in the experiment of inducing acute liver injury. CCl 4 The mechanism of liver toxicity is unanimously recognized as: CCl 4 Reductive metabolism to CCl under the action of P-450 enzyme 3 , CCl 3 ·React with unsaturated acid on the cell membrane to generate fatty acid free radicals, which can cause lipid peroxidation, damage cell membranes and organelles, and activate mechanisms such as tumor necrosis factor. For CCl 4 In the study of the induced mouse liver injury model, first select a number of healthy KM male mice, randomly group them, and infuse the corresponding drugs once a day according to the groups. After 8...

Embodiment 3

[0047] This embodiment is: take lotus leaf n-butanol extract extract 1082.32g or lotus leaf ethyl acetate extract extract 695.02g, water bath is heated to 45 ℃, light magnesium oxide (50g), talcum powder (50g) and starch (20g) added in turn and stirred evenly, spread on the baking tray, dried at below 60°C until the moisture content was below 3%, then the dried block was broken into granules below 14 mesh, and finally magnesium stearate was added (7g) and talcum powder (10g) are mixed evenly, passed through a 12-mesh sieve for granulation, pressed into 1000 tablets, packaged, and inspected for quality. Take 2 tablets three times a day per adult.

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Abstract

The invention relates to a lotus leaf extract, a preparation method and the application thereof; wherein, the extract takes the lotus leaf as raw material, the lotus leaf is quenched, filtered and decompressed by the blended solution of the acetone and water to recycle the acetone and obtain lotus leaf aqueous solution, and then the lotus leaf aqueous solution is respectively leached by ethyl acetate and normal butanol to obtain ethyl acetate extract and normal butanol extract. The ethyl acetate extract and normal butanol extract of the lotus leaf respectively lavage a mouse; carbon tetrachloride induces the liver damage of the laboratory mouse; ALT and AST value in the blood serum of the mouse and the SOD and MDA value in the hepatic tissue are detected; experiments show that the ethyl acetate extract and normal butanol extract of the lotus leaf can effectively reduce the ALT, AST and MDA value of the carbon tetrachloride hepatic tissue mouse and can obviously increase the SOD vitality of the hepatic cell of the mouse. The results show that the ethyl acetate extract and normal butanol extract of the lotus leaf have the function of reducing serum transaminase and lipid peroxidation, thereby illustrating that the ethyl acetate extract and normal butanol extract of the lotus leaf have the function of protecting the liver.

Description

technical field [0001] The present invention relates to a lotus leaf extract, and also relates to a preparation method and application of the lotus leaf extract. Background technique [0002] Liver disease and liver injury are diseases with a large number of patients and great harm to the human body. Many harmful factors can cause liver injury, mainly including viral hepatitis, alcoholic hepatitis, drug-induced hepatitis and fatty liver. The clinical manifestations Elevated serum transaminases, hepatic lobular necrosis, fatty liver, cholestasis, liver fibrosis, and can even transform into liver cirrhosis and liver cancer. The liver is the largest metabolic organ in humans, and various drugs and poisons are metabolized in the liver, so liver disease is one of the most common diseases affecting human health. International research on hepatology has been a hot spot in recent decades, but the effective prevention and treatment of liver injury is still a serious global issue. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/62A61P1/16A61K127/00
Inventor 康文艺周大鹏张伟张东娣郭曙光
Owner HENAN UNIVERSITY
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