Fluorescent mark immunity test strip detector

Abstract

The invention provides a fluorescent mark immunity test strip detector, belonging to the technical field of fluorescent mark immunity sample test strip detection. The structure comprises an optical system, a testing jig (1), a shell (8), a data collecting system and a control system; wherein the optical system comprises an excitation light source (3), a light filter (4) and a dim light CCD sensor (6); the data collecting system consists of a dim light CCD sensor data collecting circuit and a PC; the control system comprises a light reverse feed back constant light intensity automatic control circuit. Two excitation light source modes are provided in the invention, the excitation of 2-4 types of wave length can be switched, the structure is simple and reasonable, the operation is convenient and the different test requirements can be satisfied; the detailed distribution condition of the two fluorescent mark lines of a testing zone and a quality control zone on the test strip can be attained in one step; the image brightness of the CCD fluorescent mark line can be enhanced; the testing sensitivity can be improved; and the testing period of the test strip is shorter than 1min.

Description

technical field [0001] The invention belongs to the technical field of detection of fluorescently labeled immune samples, and relates to a fluorescently labeled immune test strip detector. Qualitative and quantitative detection of various target objects such as pathogenic bacteria and viruses. Background technique [0002] Due to the high sensitivity and good selectivity of fluorescence spectroscopy, the research on molecular fluorescence is very active. Santra (Santra, S.; Zhang, P.; Wang, K.; Tapec, R.; Tan, W., Anal. Chem.2001, 73, 4988.) et al once covalently coupled the mouse antihuman CD 10 antibody to the surface-modified ruthenium complex dye-doped silica nanoparticles, and then coupled the antibody particles with the single Nuclear lymphoid target cells were co-incubated. After washing away the unconjugated particles, the target leukemia cells can be labeled by the particles. Mixing non-target cells in the solution, it was found that this method has good selectiv...

AI Technical Summary

Problems solved by technology

[0004] However, at present, people generally roughly estimate the color depth of the test strips and quality control strips on the fluorescently labeled immunological test strips by visual inspection, which cannot be measured quantitatively, but only qualitatively.

In addition, because the excitation light source of the previous fluorescent-labeled immunological test strips is a single larger ultraviolet lamp, the intensity of the light source is weak, and it is difficult to concentrate the light on the test strip and the quality control strip of the fluorescent-labeled immunological test strip, resulting in The detection sensitivity is relatively low, and the requirements for the fluorescence brightness of fluorescently labeled immunoassay strips are also high

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