Fusion protein and use of fusion protein in detection of histoplasma capsulatum

Abstract

The invention discloses a fusion protein, an ELISA kit containing the fusion protein and the use of the fusion protein in the detection of histoplasma capsulatum. A DNA of a separated genome of the hiThe invention discloses a fusion protein, an ELISA kit containing the fusion protein and the use of the fusion protein in the detection of histoplasma capsulatum. A DNA of a separated genome of the histoplasma capsulatum is used as a template, and an M gene fragment and a GH17 fragment are obtained through the amplification of a PCR method; the M gene fragment and the GH17 fragment of the histoplastoplasma capsulatum is used as a template, and an M gene fragment and a GH17 fragment are obtained through the amplification of a PCR method; the M gene fragment and the GH17 fragment of the histoplasma capsulatum are inserted into a prokaryotic expression vector from a specific direction to build a M-GH17 gene fragment recombined prokaryotic expression vector; the M-GH17 recombined prokaryotic esma capsulatum are inserted into a prokaryotic expression vector from a specific direction to build a M-GH17 gene fragment recombined prokaryotic expression vector; the M-GH17 recombined prokaryotic expression vector is transferred to a host cell, and a fusion protein M-GH17 is obtained through screening, recombination, monocoloning, induction and specific expression; and the fusion protein M-GH17xpression vector is transferred to a host cell, and a fusion protein M-GH17 is obtained through screening, recombination, monocoloning, induction and specific expression; and the fusion protein M-GH17 is further used to prepare the ELLSA kit for detecting the histoplasma capsulatum. The ELISA kit containing the fusion protein M-GH17 is used in the detection of blood serum to be detected accordingis further used to prepare the ELLSA kit for detecting the histoplasma capsulatum. The ELISA kit containing the fusion protein M-GH17 is used in the detection of blood serum to be detected accordingto a common ELISA method and can detect the histoplasma capsulatum of a sample of the blood serum to be detected by determining male gender, female gender and suspicious judgment critical value accordto a common ELISA method and can detect the histoplasma capsulatum of a sample of the blood serum to be detected by determining male gender, female gender and suspicious judgment critical value according to a light absorption value after termination.ing to a light absorption value after termination.

Description

technical field [0001] The invention relates to the detection of fusion protein and capsular histoplasma, in particular to a method for detecting capsular histoplasma by enzyme-linked immunosorbent assay. Background technique [0002] Capsular histoplasmosis (Histoplasmosis, HP) is caused by the biphasic fungus Histoplasma capsulatum (Histoplasma capsulatum, HC). Since the first case of HP was reported in my country in 1958, there have been clinical reports of the disease one after another. However, because the clinical symptoms of capsular histoplasmosis are similar to those of pulmonary tuberculosis and kala-azar; the pathological forms are easily confused with Penicillium marneffei, Leishmania donovani, Toxoplasma gondii, Cryptococcus neoformans, etc. The understanding of the disease among medical workers is generally insufficient, so there is a high rate of missed diagnosis and high rate of misdiagnosis. Disseminated HP often dies due to failure to receive timely and co...

AI Technical Summary

Problems solved by technology

This method has good sensitivity and high specificity, but due to the limitation of instruments, this method cannot be widely promoted in primary medical institutions

Images