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Method for extracting and preparing high-purity ginsenoside Re from herminium by high speed counter current chromatography

A high-speed counter-current chromatography and ginsenoside technology is applied in the field of separating and preparing high-purity ginsenoside Re from ginseng fruit by high-speed counter-current chromatography, which can solve the problems of easy sample loss, slow speed and low efficiency, and achieve the effect of no sample loss.

Inactive Publication Date: 2009-09-23
孙成贺
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The separation and preparation of ginsenoside Re reported in the past mainly adopts column chromatography, which requires the use of solid fillers, which is prone to irreversible adsorption, and has the disadvantages of easy loss of samples, easy pollution, low efficiency, slow speed, high cost, and cumbersome separation steps.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Soak the pomace of ginseng fruit in 70% ethanol solution overnight, ultrasonically extract for 30 minutes, repeat the extraction three times, filter the extract and concentrate to obtain the crude extract of ginseng fruit;

[0012] Choose ethyl acetate 0.5, n-butanol 4, and water 6 as the solvent system according to the following volume ratio, place it in a separatory funnel, shake it up and then separate into layers; separate the upper and lower phases after a period of equilibrium, and the upper phase is fixed. phase, the lower phase is the mobile phase; take the crude extract of ginseng fruit and dissolve it in 2ml of the lower phase for use; use a CCC-3000 high-speed countercurrent chromatograph made in the United States to separate at room temperature and 16-28 ° C, and fill the stationary phase with For the chromatographic separation column, turn on the speed controller. When the predetermined speed is reached, the mobile phase will be pumped in at a certain flow r...

Embodiment 2

[0014] Soak the pomace of ginseng fruit in 70% ethanol solution overnight, ultrasonically extract for 30 minutes, repeat the extraction three times, filter the extract and concentrate to obtain the crude extract of ginseng fruit;

[0015] Choose ethyl acetate 2, n-butanol 7, and water 9 as the solvent system according to the following volume ratio, place it in a separatory funnel, shake it up and then separate into layers; after a period of equilibrium, separate the upper and lower phases, and the upper phase is fixed. phase, and the lower phase is the mobile phase; take the crude extract of ginseng fruit and dissolve it in 10ml of the lower phase for use; use a CCC-3000 high-speed countercurrent chromatograph made in the United States to separate at room temperature at 16-28 ° C, and fill the stationary phase with For the chromatographic separation column, turn on the speed controller. When the predetermined speed is reached, the mobile phase will be pumped in at a certain flo...

Embodiment 3

[0017] Soak the pomace of ginseng fruit in 70% ethanol solution overnight, ultrasonically extract for 30 minutes, repeat the extraction three times, filter the extract and concentrate to obtain the crude extract of ginseng fruit;

[0018] Choose ethyl acetate 1, n-butanol 6, and water 7 as the solvent system according to the following volume ratios, place the system in a separatory funnel, shake well and then separate into layers; after a period of equilibrium, separate the upper and lower phases, and the upper phase is fixed. phase, and the lower phase is the mobile phase; take the crude extract of ginseng fruit and dissolve it in 10ml of the lower phase for use; use a CCC-3000 high-speed countercurrent chromatograph made in the United States to separate at room temperature at 16-28 ° C, and fill the stationary phase with For the chromatographic separation column, turn on the speed controller. When the predetermined speed is reached, the mobile phase will be pumped in at a cer...

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Abstract

The invention relates to a method for extracting and preparing high-purity ginsenoside Re from herminium by high speed counter current chromatography, which is characterized by comprising the following steps: soaking fruit residues of the herminium in 70 percent ethanol solution through overnight; performing ultrasonic extraction for 30 minutes for three times repeatedly and concentrating extract liquid after filtration to obtain herminium coarse extract; and selecting ethyl acetate 0.5-2, butanol 4-7 and water 6-9 according to a volume ratio to form a solvent system and separating at a room temperature of from 16 to 28 by using a high speed counter current chromatographic instrument. The method for preparing and separating ginsenoside Re in large scale avoids the use of solid filler as well as irreversible adsorption, sample loss and pollution, and is high in efficiency, quick and low in cost. The obtained ginsenoside Re has a purity of more than 98 percent, and the method is suitable for separating and preparing the insenoside Re from herminium coarse extracts prepared under various process conditions.

Description

Technical field: [0001] The invention relates to a method for separating and preparing high-purity ginsenoside Re from ginseng fruit by high-speed countercurrent chromatography, which can be widely used in the separation and purification of active substances in the fields of biology, pharmacy, environmental protection, agriculture and the like. Background technique: [0002] Ginseng fruit is the mature fruit of Araliaceae plant ginseng (Panax ginseng C.A.Mey). Ginseng fruit contains various chemical components such as saponins, polysaccharides, alkaloids, sterols, volatile oils, amino acids, and inorganic elements. Pharmacological studies have shown that it has various pharmacological effects such as lowering blood sugar, anti-shock, protecting myocardium, protecting kidney function, enhancing immunity, delaying aging, and improving memory. Ginseng fruit is rich in saponins, among which Re has the highest content. Ginsenoside Re is stipulated by the "Chinese Pharmacopoeia" ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00A61K36/258
Inventor 孙成贺王英平李范英赵景辉
Owner 孙成贺
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