Fast united purification method of thyroglobulin and red kidney bean vegetable hemagglutinin

Abstract

The invention provides a method for separating and purifying red kidney bean vegetable hemagglutinin and thyroglobulin. The method mixes crude extract of red kidney bean vegetable hemagglutinin in redkidney bean with crude extract of thyroglobulin; the mixture is then extracted by coprecipitation method, purified and separated to respectively obtain hemagglutinin monomer and thyroglobulin. The method directly extracts vegetable agglutinin from red kidney bean and is applicable to obtaining of vegetable hemagglutinin without extraction of vegetable hemagglutinin after extracting of vegetable agglutinin, thereby simplifying purification process. Material used in the method is low in cost and wide in sources, and the raw materials are easy to obtain. The method has low production cost, simple operation and high yield which are applicable to industrial mass production. The vegetable hemagglutinin extracted by the method has not only high activity and high yield, but also low cost and short cycle. The method obtains hemagglutinin monomer together with thyroglobulin that the method has high product economic value, excellent generalizability, and relatively high economical and utility value.

Description

technical field

[0001] The invention relates to a new technology for extracting plant hemagglutinin monomer from red kidney beans by co-precipitation method and simultaneously purifying thyroglobulin, which belongs to the technical field of bioengineering. Background technique

[0002] Thyroglobulin is a glycoprotein found primarily in mammalian thyroid tissue. Because it can reversibly bind to lectins or glycoproteins, thyroglobulin is often used as a ligand in the field of biotechnology coupled to Sepharose 4B and other carriers for affinity chromatography separation and purification of glycoproteins. Thyroglobulin has a huge molecular weight (660kD), and the current literature reports are mainly purified by molecular sieve methods. Such as: Nubuo U etc. (Nubuo U.and Osamu T., J Biochem.1961,50 (6): 508-518) and Zeng Zhongkui Wu Qiaqing etc. (Zeng Zhongkui Wu Qiaqing, Sichuan University Journal (Natural Science Edition) 1993) use Ammonium sulfate and molecular sieves wer...