The invention discloses a method for preparing 2,4-dihydroxy-6-methoxy-3-methylacetophenone with high purity. The method comprises the following steps:
Euphorbia fischeriana is grinded and subjected to
reflux extraction by using
ethanol, then extract is filtrated to obtain a filtrate; the filtrate is condensed for recycling
ethanol to obtain a thick paste;
silica gel is added into the paste with a ratio of 5-10:1 of the
silica gel to the paste to obtain a mixture, then the mixture is blended homogeneously, dried, subjected to
column chromatography by using the
silica gel as a carrier and eluted with an eluent, and the eluent is collected; the eluent is condensed to recycle solvents to obtain crude
crystal of 2,4-dihydroxy-6-methoxy-3-methylacetophenone; the crude
crystal is dissolved into
ethanol, then silica gel is added and mixed to obtain another mixture, then the mixture is dried and subjected to
column chromatography by using silica gel for 2-3 times to obtain pure 2,4-dihydroxy-6-methoxy-3-methylacetophenone. Extraction and purification costs of the method provided by the invention can be reduced by about 20%, at the same time,
energy consumption can be significantly reduced and extraction efficiency can be increased to over 30%. The prepared 2,4-dihydroxy-6-methoxy-3-methylacetophenone has a purity of more than 99% and is especially suitable to be used as a standard substance for measuring contents of
Stellera chamaejasme medical materials and related preparations like JIEHELING tablets.