36results about How to "Purification process reduction" patented technology

The invention discloses a preparation method of tamsulosin hydrochloride with high optical purity, and belongs to a medicine technology and a chemical field. A recrystallization method is adopted, crude products of (R)-5-(2-(2-(2-ethoxyphenoxy) ethyl amino) propyl)-2-methoxyl phenyl sulfonamide hydrochloride are refined, so that pure products of the (R)-5-(2-(2-(2-ethoxyphenoxy) ethyl amino) propyl)-2-methoxyl group sulfonamide hydrochloride of which the e.e. value is larger than 99.8% is obtained; a crystallizing solvent adopted by the recrystallization method is a mixed solvent consisting of an organic solvent and water, the organic solvent is selected from one of methanol, ethyl alcohol, acetone, acetonitrile and isopropyl alcohol, and the recrystallization temperature is under 15 DEG C. The preparation method disclosed by the invention is simple to operate, short in period, low in cost and good in repeatability, and can solve the inevitable problem of rework for treatment in the industrial production.

The present invention discloses the preparation process of heat labile enterotoxin of E. coli, and the preparation process has intracellular expression or secretory expression of LT or LTB in yeast cell. Exogenously expressing LT and its mutant or subunit in eukaryotic yeast cell has greatly raised expression level. The present invention constitutes multicopy LT or LTB subunit expression kit, has increased gene dosage of LT or LTB in yeast, LT or LTB gene recombination following alcohol dehydrogenase promoter as the powerful promoter of Pichia yeast expression vector, methanol induced high expression of exogenous protein in yeast cell and thus raised expression level of LT and its mutant or LTB in yeast cell, simple post purification and safe clinical application. The present invention has low production cost and high target protein yield, and is significant in the scale production and clinical application of mucous membrane adjuvant.

The invention discloses a method and device for removing metal impurities in polycrystalline silicon by electron beam overheat smelting, and belongs to the field of metallurgy. The device comprises a water-cooled copper smelting crucible, wherein an inclined sidewall design is adopted for the water-cooled copper smelting crucible; the inner sidewall of the water-cooled copper smelting crucible forms an included angle of 105 to 120 degrees with the bottom of the water-cooled copper smelting crucible; a graphite bushing is arranged in the water-cooled copper smelting crucible; the outer surface of the graphite bushing is attached to the inner surface of the water-cooled copper smelting crucible to form a close fit design; the bottom of the graphite bushing and the bottom of the water-cooled copper smelting crucible are horizontal, the inner surface sidewall of the graphite bushing forms an included angle of 95 to 100 degrees with the bottom of the graphite bushing. According to the device, the metal impurities are removed by overheat smelting, so that the number of times of subsequent directional solidification and ingot casting can be reduced, a purification process can be eliminated, and the production cost can be reduced; by electron beam overheat smelting for polycrystalline silicon purification, post directional solidification can be reduced by one time or more, and the metal impurities in the polycrystalline silicon can be reduced by 30 percent or more.

The invention discloses a method for expressing human interleukin 7 in eucaryotic host. When the eucaryotic host is pichia X-33, the method comprises the following steps of: (1) cloning an IL-7 gene of human; (2) building an eucaryotic expression vector; (3) transforming recombinant vector to the eucaryotic yeast host; and (4) expressing a rIL-7 protein in the yeast host. A prior method for expressing IL-7 by using prokaryotic host escherichia coli is changed, and a method for expressing IL-7 through eucaryotic host pichia is searched out, thereby ensuring an IL-7 biologic activity and obtaining a large amount of stable protein.

The invention provides a method for preparing liquefied natural gas (LNG) through sulphur-tolerant methanation of coke-oven gas. The method is characterized by subjecting the coke-oven gas to direct three-stage methanation reactions on a supported molybdenum-based sulphur-tolerant methanation catalyst after removing the impurities such as oil, naphthalene, benzene and the like from the coke-oven gas, then removing the impurities such as sulphur, CO2, C2-4 hydrocarbons and the like once through rectisol, and finally separating N2, H2 and the like through one-time subzero liquefaction, thus obtaining the LNG with methane content higher than 97%. The method has the advantages of simple process flow, small equipment investment, low comprehensive energy consumption and excellent natural gas products.

The invention provides a method for separating and purifying red kidney bean vegetable hemagglutinin and thyroglobulin. The method mixes crude extract of red kidney bean vegetable hemagglutinin in redkidney bean with crude extract of thyroglobulin; the mixture is then extracted by coprecipitation method, purified and separated to respectively obtain hemagglutinin monomer and thyroglobulin. The method directly extracts vegetable agglutinin from red kidney bean and is applicable to obtaining of vegetable hemagglutinin without extraction of vegetable hemagglutinin after extracting of vegetable agglutinin, thereby simplifying purification process. Material used in the method is low in cost and wide in sources, and the raw materials are easy to obtain. The method has low production cost, simple operation and high yield which are applicable to industrial mass production. The vegetable hemagglutinin extracted by the method has not only high activity and high yield, but also low cost and short cycle. The method obtains hemagglutinin monomer together with thyroglobulin that the method has high product economic value, excellent generalizability, and relatively high economical and utility value.

The invention discloses an apparatus and a method for continuously separating o-isopropylphenol and p-isopropylphenol. The apparatus comprises: a tower 1 and a tower 2, wherein the tower 1 and the tower 2 are connected in series, rotation scrapers are respectively arranged on tower body axis centers inside the tower 1 and the tower 2, the lower portion inside the tower 1 is provided with a grid, the outer sides of the tower 1 and the tower 2 are provided with constant temperature material inlets, a liquid purification section, a crystal purification section, a melting purification section and a crystal deposition section are sequentially arranged inside the tower 1 from top to bottom, a residue liquid recovery section, a crystal re-purification section and a crystal re-deposition section are sequentially arranged inside the tower 2 from top to bottom, and an outlet on the bottom of the tower 1 is connected with the constant temperature material inlet on the middle part of the tower 2. The method comprises that: a material requiring separation is subjected to continuous re-crystallization in the tower 1 and the tower 2 to separate o-isopropylphenol and p-isopropylphenol. With the apparatus and the method, continuous material feeding and material discharging can be achieved, and high purity o-isopropylphenol and p-isopropylphenol products can be produced.

The method for yeast cell to express human interleukin 10 (hIL-10) has the technological scheme including artificially synthesizing all-gene sequence of hIL-10 including signal-carrying hIL-10 sequence, named shIL-10, and no-signal hIL-10 sequence, named nshIL-10; adding to the ends with EcorR I enzyme incising sequence and Not I enzyme incising sequence or with EcorR I enzyme incising sequences; double incising with EcorR I and Not I the integral expression vector pPIC3.5K and Ppic9K of destination gene shIL-10, nshIL-10 and Pichia yeast; constituting recombinant plasmid pPIC3.5K / shIL-10 and Ppic9K / nshIL-10; converting Pichia yeast cell via electrical perforation and plasmodic granule process; integrating recombinant plasmid and destination gene to yeast chromosome; and applying methanol to induce Pichia yeast for intracellular soluble expression and extracellular secretion type expression. The present invention is superior to expression in colibacillus.