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A kind of preparation method of slow-release microcapsule and composite membrane for inhibiting peach brown rot bacteria

A brown rot fungus and composite membrane technology, applied in the directions of botanical equipment and methods, chemicals for biological control, fungicides, etc., can solve the problems of polluted environment, short-term efficacy, economic loss, etc.

Inactive Publication Date: 2011-12-21
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Peach brown rot is a fungal disease that damages the flowers, leaves, branches and fruits of peach trees, especially the fruits, causing a large number of fruit drop and rot, causing huge economic losses
Traditional chemical pesticides are gradually banned due to environmental pollution and bacterial resistance
At present, there are no natural pesticides that can effectively control peach brown rot and are environmentally friendly at home and abroad.
In addition, traditional dosage forms cannot meet the requirements of modern pesticides due to their short-term efficacy. Therefore, it is urgent to screen natural active substances that can effectively inhibit peach brown rot fungus and be environmentally friendly, and develop new dosage forms to prolong the drug effect time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] Preparation of slow-release microcapsules inhibiting peach brown rot:

[0019] 1) Weigh chitosan and berberine, the mass ratio of chitosan and berberine is 9-11: 0.22-0.28, add acetic acid solution, after chitosan and berberine fully swell, stir to dissolve, Stand still, remove bubbles, obtain berberine-chitosan solution, standby;

[0020] 2) Add the berberine-chitosan solution dropwise into soybean oil containing Span 80, stir at a constant temperature of 40-50°C, and emulsify for 28-32 minutes to form a W / O system;

[0021] 3) Add glutaraldehyde solution dropwise, stir for 2.5-3.5 hours, and centrifuge to make the microcapsules settle completely;

[0022] 4) Pour out the upper preparation solution, wash the obtained microcapsules with petroleum ether for 3-5 times, and vacuum-dry the obtained microcapsules at 48-52° C. for 23-25 ​​hours to obtain the final product.

[0023] Microcapsule slow-release and antibacterial function: use pH6.8 phosphate buffer (take 250 mL...

Embodiment 1

[0028] Weigh chitosan 9g and berberine 0.22g, add 0.18wt% acetic acid solution 9mL and wait for it to fully swell, stir to make it dissolve, let stand, remove air bubbles, obtain berberine-chitosan solution, set aside; Add the berberine chitosan solution dropwise to 68mL soybean oil containing 0.18wt% Span80, stir at a constant temperature of 40°C, and emulsify for 28min to form a W / O system; add 1.1mL 48wt% glutaraldehyde solution dropwise , stirred for 2.5 hours, and centrifuged to make the microcapsules settle completely; pour the upper preparation solution, wash the obtained microcapsules with petroleum ether for 3 times, and vacuum-dry the obtained microcapsules at 48°C for 23 hours to obtain the final product.

[0029] The results showed that the initial release rate of the drug was fast, reaching 16% in the first day, and then the release rate slowed down. The accumulative release rate of berberine reached 72% in one month, and the inhibitory rate of the drug to pathogen...

Embodiment 2

[0031] Weigh chitosan 10g and berberine 0.25g, add 0.18wt% acetic acid solution 9mL and wait for it to fully swell, stir to make it dissolve, let stand, remove air bubbles, obtain berberine-chitosan solution, and set aside; Add berberine chitosan solution dropwise into 68mL soybean oil containing 0.18wt% Span80, stir at a constant temperature of 45°C, and emulsify for 30min to form a W / O system; add 1.1mL 48wt% glutaraldehyde solution dropwise , stirred for 3 hours, and centrifuged to make the microcapsules settle completely; pour the upper layer preparation solution, wash the obtained microcapsules with petroleum ether for 3 times, and vacuum-dry the obtained microcapsules at 50°C for 24 hours to obtain the final product.

[0032] The results showed that the initial release rate of the drug was fast, reaching 17% in the first day, and then the release rate slowed down. The accumulative release rate of berberine reached 76% in one month, and the inhibitory rate of the drug to p...

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Abstract

The invention discloses a preparation method of a slow-release microcapsule and a composite film for inhibiting peach brown rot fungus. Preparation method of sustained-release microcapsules: weigh chitosan and berberine, the mass ratio of chitosan and berberine is 9-11:0.22-0.28, add acetic acid solution, and wait for chitosan and berberine to fully swell Finally, stir to dissolve, let it stand, and remove air bubbles to obtain a berberine-chitosan solution, add it to soybean oil containing Span 80, stir at a constant temperature of 40-50°C, and emulsify for 28-32 minutes to form W / O system; glutaraldehyde solution, stirred for 2.5-3.5 hours, centrifuged to make the microcapsules settle completely; decant the upper preparation solution, and wash the microcapsules with petroleum ether for 3-5 times, and store the microcapsules at 48-52°C Vacuum dry for 23-25 ​​hours. The invention also provides a preparation method of the slow-release composite film for inhibiting the peach brown rot fungus. Both the microcapsule and the composite film of the present invention have sustained-release effects, and because the berberine is a natural compound, it has no toxic and side effects on the environment and plants.

Description

technical field [0001] The invention belongs to the field of fungicide production, and relates to a method for preparing slow-release microcapsules and a composite film of berberine (also known as berberine) extracted from traditional Chinese medicine on peach brown rot fungus (Monilinia fructicota). Background technique [0002] Screening antifungal active substances from medicinal plants is an important direction in the research and development of fungicides. my country has rich resources of medicinal plants, drawing on the research results of traditional Chinese medicine, it is expected to screen out natural active substances against phytopathogenic fungi from medicinal plants. These active substances can be directly used as natural fungicides, or used as lead compounds to develop highly active fungicides through structural modification. [0003] Peach brown rot is a fungal disease that damages the flowers, leaves, branches and fruits of peach trees, especially the fruit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01N25/34A01N25/28A01P3/00A01N43/90
Inventor 田平芳侯东耀阎春琦葛喜珍
Owner BEIJING UNIV OF CHEM TECH
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