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Microbial enzymes racemization preparation method of DL-alanine

A microbial enzyme and alanine technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high equipment conditions, difficult separation and high production costs, and achieve a simple and easy production process. , The effect of high chemical purity and low manufacturing cost

Active Publication Date: 2012-07-18
淮北新旗氨基酸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Disadvantages of this method: the use of severe chemical raw material cyanide, long synthesis route, reaction mixture contains a large amount of ammonia ions and chloride ions, difficult separation, high production cost, serious pollution, and high requirements for equipment conditions
[0008] Disadvantages of this method: use severe chemical raw material cyanide, long synthesis route, reaction mixture contains a large amount of ammonia ions and sodium ions, difficult to separate delicately, high production cost, serious pollution, high requirements for equipment conditions
[0011] Disadvantages of this method: the use of dangerous chlorine gas, hydrochloric acid as a by-product in the production process, the product contains a large amount of ammonium chloride, which needs to be removed by ion exchange, the product quality is unstable, the production cost is high, and the pollution is serious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Prepare medium 1 containing peptone 20g / L, L-lactic acid 15g / L, sodium chloride 5g / L, corn steep liquor powder 18g / L glycerin 5g / L, magnesium sulfate 0.2g / L, potassium dihydrogen phosphate 1g / L liter, adjust the pH value to about 7 with ammonia or sulfuric acid, and put it into 1000 ml Erlenmeyer flasks, with a liquid volume of 250 ml per bottle. The above medium was autoclaved at 121°C for 15 minutes. Cool to 30°C, pick a ring of Escherichia coli stored on the slant with a ф1mm inoculation loop and insert it into the above medium. Incubate at 30° C., 110 rpm for 24 hours using a rotary shaker.

[0026] Combine 1 liter of the above-mentioned cell culture solution, add to 10 liters of substrate solution containing 1600 g L-alanine, maintain the reaction temperature at 37 degrees, and the pH value at 8. The specific rotation of the reaction system was measured with a polarimeter every hour during the reaction, and the reaction was continued for 1 hour when the specific ...

Embodiment 2

[0028] The preparation contains peptone 20g / L, yeast extract 20g / L, L-alanine 20g / L, sodium chloride 5g / L, corn steep liquor powder 18g / L, magnesium sulfate 0.2g / L, potassium dihydrogen phosphate 1g / L 150 milliliters of the primary culture medium, adjust the pH value to about 7 with ammonia water or sulfuric acid, divide into 500 milliliter Erlenmeyer flasks, and the volume of each bottle is 150 milliliters. The above medium was autoclaved at 121°C for 15 minutes. Cool down to 37°C, and use a ф1mm inoculation loop to pick out a loop of the Escherichia coli stored on the slant and insert it into the above medium. Using a rotary shaker, cultivate at 30, 110 rpm for 16 hours to obtain 400 ml of primary seed cell suspension.

[0029] Preparation containing peptone 20g / L (dried), yeast extract 20g / L, L-lactic acid 20g / L, sodium chloride 5g / L, corn steep liquor powder 5g / L, magnesium sulfate 0.2g / L, potassium dihydrogen phosphate 10 liters of fermentation broth with 1g / L and L-ala...

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Abstract

The invention relates to a microbial enzymes racemization preparation method of DL-alanine, comprising the following steps: preparing a substrate, adjusting the PH value of the substrate to eight by ammonia or sulphuric acid, canning the obtained substrate in flasks, inoculating microorganisms saved on an inclined plane to the substrate by picking one loop of microorganisms with a transfering loop, culturing the substrate for 24h at 30 DEG C by a rotary shaker of which the rotation rate is 110rpm, merging the above cell supernatant, adding the cell supernatant in a substrate solution, reacting at 30 DEG C at PH value of 7.0 when a polarimeter is used to measure the specific rotation of the reaction system every hour, continuing to react when the specific rotation is zero and heating up to60-100 DEG C, destaining for 30min by adding active carbon, filtering, and vacuum concentrating and crystallizing by a rotary film evaporator to obtain the DL-alanine finished goods. The invention has the advantages of accessible raw materials, simple production process and mild conditions, and can greatly reduce the production cost of DL-alanine.

Description

technical field [0001] The present invention relates to a method for producing DL-alanine. Background technique [0002] At present, the production process of DL-alanine at home and abroad mainly uses the reaction of acetaldehyde and hydrocyanic acid to generate cyanohydrin, which is reacted with ammonia to obtain cyanamide, which is hydrolyzed under alkaline conditions to generate amino acid sodium, which is The crude product of aminopropionic acid was obtained by exchange, and the finished product was obtained by recrystallization with water or ethanol. Alanine can also be synthesized from raw materials such as ether, ammonium chloride, and sodium cyanide. However, these synthetic methods all use cyanide, which is highly toxic, difficult conditions, serious waste problems, long synthetic routes and low yields. In addition, aminopropionic acid can also be produced by the chlorination method. This method uses propionic acid chlorination to obtain chloropropionic acid, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/06C12R1/645C12R1/44C12R1/225C12R1/145C12R1/38C12R1/19
Inventor 黄建坡黄勇尹若春
Owner 淮北新旗氨基酸有限公司
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