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Method for adding ethanol to enhance beta-cyclodextrin yield

A cyclodextrin and yield technology, which is applied in the fields of polymer compound inactive ingredients, food preparation, food science, etc., can solve the problems of limiting the use range of cyclodextrin, long reaction time, large energy consumption, etc. The process of recovering organic solvents, the effect of less ethanol residue and simple operation

Inactive Publication Date: 2012-11-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this process is that secondary liquefaction and recovery of organic solvents are required in the production process, there are multiple heating and cooling processes, the reaction time is long, and the energy consumption is large; the organic solvents used in this process are usually toxic reagents (cyclohexane, toluene, etc. ), the residual organic solvents in the product limit the scope of use of cyclodextrin, especially in food and medicine, and have a certain impact on the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] This example respectively gives the comparison of the yield of β-cyclodextrin obtained without adding ethanol, adding complexing agent cyclohexane, and adding ethanol.

[0014] The mass concentration of cornstarch milk is 30g / 100g, the pH is 7.5, the enzymatic reaction temperature is 60°C, the amount of cyclodextrin glucosyltransferase is 5 enzyme activity units per gram of starch, and the conversion time is 12 hours.

[0015] Table 1: Comparison of yields of β-cyclodextrin by three production methods

[0016] method

1

2

3

Yield

29.67％

50.87％

60.21％

[0017] Method 1 is a process in which ethanol is not added during the production process.

[0018] Method 2 is the process of adding complexing agent cyclohexane in the production process, and the addition amount of complexing agent cyclohexane is 0.4mL / g starch.

[0019] Method 3 is a new process of adding ethanol in the production process of the present inven...

Embodiment 2

[0021] Configure cornstarch milk with a mass concentration of 15g / 100g, stir at a high temperature of 80°C for 60 minutes, then adjust the temperature to 56°C, add cyclodextrin glucosyltransferase at a concentration of 6 enzyme activity units per gram of starch, and then Add ethanol 0.5mL / g starch, fully react for 12 hours, adjust the pH value in a conventional way during the conversion process, the pH is controlled at 8, and obtain β-cyclodextrin by crystallization after filtration, the final yield of β-cyclodextrin was 63.32%.

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PUM

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Abstract

A method for adding ethanol to enhance the beta-cyclodextrin yield belongs to the cyclodextrin production technology field. The invention utilizes the cyclodextrin glycosyltransferase to catalyze the starch to transform and produce the beta-cyclodextrin, adds the ethanol in the reaction liquid to improve the beta-cyclodextrin yield. The method comprises: blending the starch milk with the mass concentration of 15-30%; stirring for 30-120 minutes at 80-90 DEG C, regulating the temperature to 50-60 DEG C, adding the cyclodextrin glycosyltransferase based on the concentration of 3-8 U in 1g starchand adding ethanol; completely reacting for 8-12 hours after the ethanol adding quantity achieves 0.1mL-0.5mL in 1g starch, regulating pH value to 6-9 in the transforming process, filtering, crystallizing and obtaining the beta-cyclodextrin products. The method has no process for recovering the organic solvent and greatly enhances the beta-cyclodextrin yield. The product beta-cyclodextrin has noorganic solvent residues.

Description

technical field [0001] The invention discloses a method for increasing the yield of β-cyclodextrin by adding ethanol, which belongs to the technical field of cyclodextrin production by using starch as a raw material and adding ethanol to a reaction solution to increase the yield of β-cyclodextrin. Background technique [0002] Cyclodextrin (CD) is a type of cyclic oligosaccharide produced by the action of cyclodextrin glycosyltransferase (CGTase) produced by certain species of Bacillus on starch. Cyclodextrin has a stereochiral cavity with a hydrophilic outer ring and a hydrophobic inner ring with a certain size. This special structure makes it have many special physical and chemical properties. Its ring structure and cavity can envelop different compounds, such as organic molecules, inorganic compounds, etc., to form clathrates, which can be used as quality improvers, stabilizers, drug carriers and adsorbents in food, medicine, chemical industry and environmental protection...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/18A61K47/40A23L1/09A23L29/30
Inventor 顾正彪程力洪雁王亮李兆丰
Owner JIANGNAN UNIV
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