Reagent for separating RNA from biological tissue/cell/blood sample

Abstract

The invention provides a reagent for separating RNA from a biological tissue / cell / blood sample, which is prepared from the following raw materials by weight: 76.424 percent of guanidine hydrochloride, 0.4627 percent of dithiothreitol, 0.2723 percent of sodium thiosulfate, 42 percent of sodium bicarbonate, 4.7 percent of phenol water, and water added to 100 percent of weight proportion. The reagent can be stably stored for long term under a room temperature environment. The inventor carries out a contrast experiment on the reagent and an imported TRIzol reagent, and experiment results prove that the two products have similar stability; the obtained total RNA electrophoresis through extraction has uniform banding pattern; the ratio of OD260 / OD280 has no significant difference; reverse transcription-polymerase chain reaction and other downstream experiments carried out by taking the extracted RNA as a template obtain satisfied effects; and product cost is reduced. By using the reagent, RNA can be separated and extracted from liver tissue, brain tissue, blood and bile of an animal, and RNA can be separated and extracted from tissues of a plant.

Description

technical field [0001] The invention belongs to the compound containing two or more single nucleotide units, which has the technical field of independent phosphate or polyphosphate groups connected by nucleoside-based sugar compound groups, and specifically relates to the technical field of using ribose groups as sugar group. Background technique [0002] In eukaryotes, RNA is the intermediate carrier of genetic information and participates in processes such as protein synthesis and gene expression regulation. For some viruses (RNA viruses), RNA is the only carrier of genetic information. With the continuous development of molecular biology research technology, in-depth research on life events at the RNA level has gradually become a research focus in the field of life sciences. RNA extraction technology is not only an important part of molecular biology technology, but also an important basis for functional genomics research technology. Studying the regulatory mechanism of...

AI Technical Summary

Problems solved by technology

Phenol is easily oxidized and degraded when exposed to sunlight and air, and its stability can only be maintained by storing it in an environment below -20°C or adding a protective agent (8-hydroxyquinoline), which limits the use of phenol in RNA extraction experiments

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