Method for extracting L-proline from fermentation liquor by membrane separation technology

Abstract

The invention discloses a method for extracting L-proline from fermentation liquor by a membrane separation technology. The method comprises the following steps: firstly performing microfiltration, ultrafiltration and reverse osmosis concentration on the fermentation liquor of the L-proline to obtain reverse osmosis dialyzate, evaporating and concentrating the reverse osmosis dialyzate by a conventional method, and then crystallizing at the reduced temperature to obtain a crude product L-proline. The method helps fundamentally solve the problem of environmental pollution during the extraction process of the L-proline, reduce production cost, increase economic benefit, improve the product grade and promote sustainable development of production of the L-proline.

Description

technical field [0001] The invention belongs to the technical field of amino acid production and relates to a method for extracting L-proline from fermented liquid by membrane separation technology. Background technique [0002] L-proline is a neutral amino acid containing imino group, belongs to heterocyclic amino acid, is one of the 20 basic amino acids that make up protein, and is a non-essential amino acid for human body. L-proline has important uses in pharmaceutical, chemical and agricultural industries. In medicine, it is mainly used as one of the raw materials of compound amino acids. It is used for protein supplementation after malnutrition, protein deficiency, severe gastrointestinal diseases, scalds and surgical operations. In recent years, L-proline has been widely used as an intermediate in drug synthesis. It is widely used in the research and development of new drugs; in the synthesis industry, L-proline can participate in the induction of asymmetric reactions...

AI Technical Summary

Problems solved by technology

The pentachlorophenol salt-forming precipitation method is to use L-proline and pentachlorophenol to form a salt, precipitate and precipitate, and then analyze and precipitate with ammonia water to obtain L-proline, but pentachlorophenol is a highly toxic substance, and the operating environment is difficult to guarantee ; Alumina column chromatography is generally used for the refinement of products and the purification of crude products. Because of its small scale, it is only limited to the scope of the laboratory, and it cannot be industrialized at present; The extraction of amino acid, but because the amount of wastewater generated by the extraction of L-proline by ion exchange method is too large, the environmental protection problem is difficult to solve