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Bacillus subtilis and application thereof in biological deodorant

A technology of Bacillus subtilis and biological deodorant, which can be applied to Bacillus subtilis and its application field in biological deodorant, and can solve the problems of weak degradation ability of organic matter and the like

Inactive Publication Date: 2010-02-03
惠飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, all biological deodorizers that appear on the market can only degrade sulfides (such as hydrogen sulfide) and ammonia, and have weak degradability to organic matter (total hydrocarbons).

Method used

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  • Bacillus subtilis and application thereof in biological deodorant
  • Bacillus subtilis and application thereof in biological deodorant
  • Bacillus subtilis and application thereof in biological deodorant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] In a sterile ultra-clean workbench, use an inoculation loop to scrape 3 rings of the slant strain of Bacillus subtilis HF-A1 (CGMCC No.3230), and inoculate it into the first-level liquid seed medium (glucose: 15, yeast extract: 10, Peptone: 10, CaCl 2 : 3, KH 2 PO 4 : 5, Na 2 HPO 4 12H 2 O: 4, MgSO 4 ·7H 2 O: 6, pH7.0, unit: g / L, 115°C, sterilized for 20min). Shake culture at a temperature of 30° C. and a rotational speed of 150 rpm for 48 hours to obtain a grade 1 seed liquid. The primary seed liquid is transferred to a sterilized medium containing the same components according to the inoculum amount of 10% and cultivated under the same conditions, and the secondary seed liquid can be obtained after 48 hours.

[0044] Inoculate the secondary seed liquid into 35L sterilized liquid medium according to the inoculation amount of 10% (the volume of the fermenter is 50L), glucose: 30, beef extract: 10, peptone: 8, CaCl 2 : 5, KH 2 PO 4 : 8, Na 2 HPO 4 12H 2 O: ...

Embodiment 2

[0045] Example 2 Indoor Garbage Deodorization Test

[0046] In a sealed room, put a pile of highly rotten garbage that produces stench, and this biological deodorant (the fragrance-free biological deodorant prepared according to the method of Example 1) is diluted 10 times with tap water before use , Spray 200ml with a spray gun, and try to make the spraying range of deodorant spread over the garbage dump area. Set up different collection sites around the garbage for sampling, and measure the H in the malodorous gas before spraying and 15 minutes after spraying 2 S and NH 3 Concentration, the sampling point is 1m away from the edge of the odor source, the sampling height is 1.3m, 5 points are sampled, and the average value is taken. Odor concentration adopts three-point comparative smelly bag method (GB / T14675); ammonia adopts sodium hypochlorite-salicylic acid spectrophotometry (GB / T14679); hydrogen sulfide adopts polyvinyl alcohol ammonium phosphate absorption-methylene bl...

Embodiment 3

[0050] Embodiment 3 deodorizing effect and cost comparison

[0051] Divide a pile of garbage into four equal parts, place them in four rooms with the same area and space volume, and spray four kinds of deodorants respectively. According to the method of Example 2, the difference in the deodorizing effect of different brands of deodorants with the same volume and the same dilution was investigated, and the results are shown in Table 3. Wherein, the biological deodorant of the present invention is a fragrance-free biological deodorant prepared according to the method of Example 1.

[0052] Table 3. Comparison of deodorization and cost between this biological deodorant and common products in the market

[0053]

[0054]

[0055] As can be seen from Table 3: the biological deodorant provided by the present invention is obviously superior to other deodorants in cost and deodorizing effect. Embodiment 4 to the deodorization of domestic sewage

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Abstract

The invention belongs to the field of environment protection and discloses a new bacillus subtilis and the application thereof in biological deodorant. The bacillus subtilis HF-A1 related in the invention is preserved in Common Microorganism Center, China Committee of Culture Collection for Microorganisms on August 19th, 2009, with a preservation number of CGMCC No. 3230. A strain of the bacillussubtilis is obtained by selecting samples collected from a plurality of ecological environments. The strain has an obvious deodorizing and bacteriostatic effect in various stinky environments; averageNH3 removal rate can be as high as 95.5 percent; average H2S removal rate can be as high as 93.8 percent; average total hydrocarbon removal rate can be as high as 94.3 percent; average total bacteriaremoval rate can be as high as 87.9 percent; average odor concentration removal rate can be as high as 96.8 percent; and the new bacillus subtilis can be used for deodorizing and purifying garbage, domestic sewage, animal house air and the like.

Description

technical field [0001] The invention belongs to the field of environmental protection and relates to a new bacillus subtilis and its application in biological deodorants. Background technique [0002] Stench is a kind of sensory public hazard with many sources and a wide area of ​​pollution. It not only makes people feel unpleasant and disgusting, but also many substances in the odor directly endanger human health. For this reason, since the 1960s, it has gradually attracted the attention and attention of environmental science and technology workers at home and abroad, and has achieved various results. Japan promulgated the "Odor Control Act" in 1971, the United States promulgated the "Clean Air Act" in 1971, and Germany stipulated the frequency and time measurement methods of odor in the "Ordinance on Odor in the Ambient Atmosphere" in 1993 . my country's research on odor pollution started relatively late. With reference to Japan's experience, the "Odor Pollutant Emissio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84C12R1/125
CPCY02A50/20
Inventor 刘小勇王有智惠飞王大伟魏晶惠大帅肖淑红翁万山
Owner 惠飞
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