Extraction method of raspberry for preventing primary carcinoma of liver

A primary liver cancer and extraction method technology, applied in the field of raspberry extraction to prevent primary liver cancer, can solve the problems of loss of anti-oxidation, imbalance of compatibility, low concentration of extracts, etc., to ensure the ability of anti-oxidation and reduce color Browning, high concentration effect

Active Publication Date: 2010-03-03
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The process of extracting raspberries by the School of Food Science and Technology of Shenyang Agricultural University is carried out by SephadexG-100 column chromatography. The loss of neutral groups and weakly charged groups, as we all know, many organic substances are neutral; secondly, due to the compatibility between phytochemicals, the chromatography method cannot fully extract the active ingredients in raspberries, which leads to Due to the imbalance of compatibility, the efficacy of phytochemicals cannot be fully exerted; again, chromatography requires good fluidity of the solution, resulting in a very low concentration of the extract, which is not conducive to studying the effect of the high concentration group
[0005] The Department of Applied Chemistry, College of Science, Northeast Agricultural University has studied the process of extracting raspberry polysaccharides by hot water method. This method also has obvious disadvantages: first, the antioxidant components in raspberries are organic matter, and water cannot fully extract them; second, Antioxidant ingredients in raspberry will be oxidized in hot water without effective measures, thus losing the ability to resist oxidation, and there will be no anti-tumor ability of raspberry extract

Method used

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Examples

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Embodiment 1

[0014] The method for extracting raspberries to prevent primary liver cancer comprises: picking fresh raspberries in the ripe season and breaking them to remove slag, fully stirring the crushed and slag-removed raspberries, putting them in a beaker and placing them together On ice, add 80% acetone 200ml to 100g raspberries and mix in a pre-cooled mixer for 3 minutes, then use a homogenizer to continue homogenizing and stirring for 2 minutes, take out the homogenate and put it into a filter connected to a vacuum pump In the bottle, put Whatman2# filter paper on the filter bottle, collect the filtrate and place it in a rotary evaporator and rotate it at 50°C for 45 minutes, adjust it to a final volume of 50ml with deionized water, and place it at minus 20 ℃-minus 80 ℃ frozen storage.

[0015] TOSC test is to use gas chromatography analysis method to detect the oxidation resistance of purified solutions or biological samples. In the presence of oxygen, ABAP can generate peroxide...

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Abstract

The invention discloses an extraction method of raspberry for preventing primary carcinoma of liver. The prior extraction method has many defects. The extraction method of the raspberry for preventingprimary carcinoma of liver comprises: picking fresh raspberry in a ripening season, grinding the fresh raspberry and removing slag; fully stirring the ground raspberry from which the slag is removed,filling he ground raspberry in a breaker and placing the breaker on an ice block; adding 200 milliliters of 80 percent acetone in 100 grams of raspberry, and mixing the mixture in a pre-cooled mixerfor 3 minutes; continuously homogenizing and stirring the mixture by using a homogenizer for 2 minutes; filling homogenate in a filter flask which is connected with a vacuum pump and provided with filter paper; collecting filtrate, filling the filtrate in a rotary evaporator and performing rotary evaporation at 50 DEG C for 45 minutes; adjusting the volume of the filtrate with deionized water to afinal volume of 50 milliliters; and placing the solution at 20 DEG C below zero to 80 DEG C below zero for refrigeration. The method is an extraction method of raspberry.

Description

Technical field: [0001] The invention relates to a raspberry extraction method for preventing primary liver cancer. Background technique: [0002] Raspberry (Raspberry), also known as raspberry, is a plant of the genus Rubus (Rubus sp.) in the Rosaceae. -Aminobutyric acid and other anti-aging substances and anti-cancer substances -Ellagic acid (Ellagic acid), the content of vitamin E and tannic acid is the highest among all fruits. The United States claims that red raspberry is the killer of cancer and is known as "ruby". Raspberry is a high-nutrition emerging fruit that is popular in Europe and America and is welcomed by people. Tannic acid has special effects on colon, cervical, breast, and pancreatic cancer cells. Raspberry and its processed products can effectively maintain the active ingredients of tannic acid and can be absorbed by the body. Raspberry is the food with the highest natural tannic acid content found today. Tannic acid is a phenolic compound with a ben...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P1/16A61P35/00A61K131/00
Inventor 刘明宋文婷张春鹏林罗强赵金璐
Owner HARBIN MEDICAL UNIVERSITY
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