Method for microwave extracting fat-soluble toad venom by using skin of Bufo bufo gargarizans

Abstract

The invention discloses a method for microwave extracting fat-soluble toad venom by using skin of Bufo bufo gargarizans, which comprises the following steps: using ethanol as extraction solvent, using microwave to dispel, heat and extract, using ultrafiltration membrane to filter and then concentrating the extraction solvent, and using liquid chromatogram technique to conduct separation and purification, wherein the extraction temperature breaks through the convention, is relatively higher but is still controlled within the lowest denaturation temperature, and the extraction time is shorter and less than one hour. Therefore, the denaturation of the fat-soluble toad venom is less, and the extraction rate is relatively higher during the extraction process; when the liquid chromatogram conducts separation and purification, the retention time of bufalin, cinobufagin and resibufogenin are respectively 7.594min, 10.103min and 10.741min, thus being capable of better separating and obtaining various fat-soluble toad venoms; the obtained bufalin, cinobufagin and resibufogenin has high purity which can reach 99%, and the impurity is little, therefore the obtained various fat-soluble toad venoms are more stable, and not easy to generate denaturation; and the extraction rate of the fat-soluble toad venom is more than 17%, and the highest extraction rate can reach 40%.

Description

technical field [0001] The invention relates to a method for extracting toad venom, in particular to a method for using toad skin to extract fat-soluble toad venom by microwave. Background technique [0002] Bufo Bufo gargarizans Cantor (Bufo bufo gargarizans Cantor) or Bufomelanostictus Schneider (Bufomelanostictus Schneider) is a white slurry secreted by the glands behind the ears and skin of Bufo bufo gargarizans Cantor, which is processed and dried. Its nature and flavor are sweet, pungent and warm, and poisonous. It has the effects of detoxification, swelling, refreshment, resuscitation, cardiac strengthening and anti-tumor. Toad contains a variety of chemical components, which are mainly divided into fat-soluble parts and water-soluble parts. Fat-soluble bufafoat include bufoginin, cinobufagin and bufolin. At present, the method of extracting toad venom is mainly to reflux extraction with an organic solvent under heating conditions. Commonly used organic solvents ar...

AI Technical Summary

Problems solved by technology

Such as the Chinese invention patent application whose application number is 200610070105.X, it is disclosed that the weight is 9 times or 10 times the weight of dry toad skin with 80% or 95% ethanol to extract for 90 minutes or 120 minutes, and then use macroporous resin column adsorption separation , eluted step by step with water, low-concentration ethanol, and high-concentration ethanol, then extracted with ethyl acetate, and dried to obtain the fat-soluble toad venom extract, but the steps of this extraction method are complicated, and the extraction rate of fat-soluble toad venom Longer, high denaturation rate of fat-soluble toad venom, and lower purity of fat-soluble toad venom obtained by resin separation

In addition, the Chinese invention patent application with the application number 00136668.8 discloses digestion and extraction with microwave heating, the extraction temperature is 30-60°C, and the extraction time is 1-10 hours. Toad venom extract is obtained by drying. The extraction time is longer, the extraction temperature is lower, and the extraction rate is correspondingly lower. After extraction, there is no separation and purification.