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Compound panax ginseng total saponin and levamisole nano emulsion adjuvant and preparation method thereof

A technology of ginsenoside and levamisole, which is applied in the field of compound ginsenoside and levamisole oil-in-water nanoemulsion adjuvants, can solve the problems of poor stability and difficult preparation technology, achieve stable properties, improve cellular immune function, and Convenience effect

Inactive Publication Date: 2010-03-17
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The water-in-oil-in-water emulsion not only reduces the viscosity but also has good immunogenicity, but the stability of the system is poor, and the preparation technology is difficult

Method used

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  • Compound panax ginseng total saponin and levamisole nano emulsion adjuvant and preparation method thereof
  • Compound panax ginseng total saponin and levamisole nano emulsion adjuvant and preparation method thereof
  • Compound panax ginseng total saponin and levamisole nano emulsion adjuvant and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Accurately weigh 40.00g of polyoxyethylene ether (40) hydrogenated castor oil, 1.00g of glycerin, 1.64g of isopropyl myristate, 1.00g of total ginsenosides, 0.10g of levamisole hydrochloride and 56.26g of distilled water, set aside ;

[0033] 2) Stir and mix polyoxyethylene ether (40) hydrogenated castor oil, glycerin and isopropyl myristate at 37°C, then add ginsenoside into it and dissolve it, and the obtained oil phase;

[0034] 3) At room temperature, completely dissolve levamisole in distilled water, let it stand for 1 hour, and filter to obtain an aqueous phase.

[0035] 4) At room temperature, slowly add the water phase into the oil phase dropwise, and continuously stir while adding dropwise until a transparent system is formed, and filter to obtain the light yellow nanoemulsion adjuvant of the present invention.

Embodiment 2

[0037] Component Content

[0038] Total Ginsenosides 1.50%

[0039] Levamisole Phosphate 5.00%

[0040] Polyoxyethylene ether (40) hydrogenated castor oil 17.50%

[0041]Glycerin 17.50%

[0042] Isopropyl myristate 5.00%

[0043] Distilled water 53.50%

Embodiment 3

[0045] Component Content

[0046] Ginsenoside Rb1 1.00%

[0047] Levamisole Hydrochloride 2.00%

[0048] Polyoxyethylene ether (40) hydrogenated castor oil 15.00%

[0049] Glycerin 15.00%

[0050] Peanut Oil 8.80%

[0051] Distilled water 58.20%

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Abstract

The invention discloses a compound panax ginseng saponin and levamisole nano emulsion adjuvant. The adjuvant has the particle diameter of 1-100 nm and comprises panax ginseng saponin, levamisole, a surface active agent, a cosurfactant, oil and distilled water. The product is transparent faint yellow liquid in appearance, and the particle parameter is smaller than 100 nm; the viscosity is lower, and the viscosity requirement of emulsion as a vaccine adjuvant is met; the stability is higher, and the product can be preserved at room temperature or 4 DEG C for a long time; the special emulsification is not needed during seedling preparation, the requirement to equipment is low, and the quality of the product is easy to control. Egg white albumin (OVA) as mode antigen indicates that the compound panax ginseng saponin and levamisole nano emulsion adjuvant can induce better immune reaction than an aluminum adjuvant in a certain dosage, generate higher antibody titer, reduce the dosage of theadjuvant or the antigen, simulate Th1 and Th2 reaction, reinforce the body fluid and cell immunization of an organism and play a role of complete immune protection.

Description

technical field [0001] The invention relates to the field of vaccine adjuvants, in particular to a compound ginsenoside and levamisole oil-in-water nanoemulsion adjuvant which can form a composition with an antigen, has the immune effect of enhancing the antigen, and is used for the prevention and treatment of animal diseases. Background technique [0002] In recent years, with the occurrence of some animal diseases or zoonotic diseases, the development of new vaccines is very difficult. The development of science and technology has changed the production of vaccines from complete microbial vaccines to subunit vaccines or synthetic peptide vaccines containing only microbial specific components, but the immunogenicity of the latter is relatively weak, and it is difficult to induce an effective immune response in the body . In order to enhance the immunogenicity of vaccines, immune adjuvants are often added in the development of viral vaccines, DNA vaccines and polypeptide va...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K9/107A61K47/44A61K47/34A61K47/06A61K47/10A61K47/26
Inventor 欧阳五庆曹发昊李雅
Owner NORTHWEST A & F UNIV
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