Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for producing xylo-oligosaccharide and xylose by using immobilized enzyme

A technology of xylo-oligosaccharides and immobilized enzymes, applied in directions such as immobilization on/in organic carriers, fermentation, etc., can solve the problems of unreported research on application and few researches on application, so as to improve the utilization rate, Good economy and the effect of improving utilization efficiency

Inactive Publication Date: 2010-03-17
CHINA AGRI UNIV
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above studies mostly focus on the immobilization of xylanase, and there are few studies on specific applications.
In addition, the existing research is mainly about the immobilization of xylanase, and there is no report on the study of immobilized xylanase-xylosidase and its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing xylo-oligosaccharide and xylose by using immobilized enzyme
  • Method for producing xylo-oligosaccharide and xylose by using immobilized enzyme
  • Method for producing xylo-oligosaccharide and xylose by using immobilized enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, take corn cob steam explosion liquid as raw material preparation xylo-oligosaccharide

[0028] 1. Preparation of corn cob steam explosion liquid

[0029] Weigh 100g corncobs (20-40 mesh) (containing 32.4% xylan), add 800mL distilled water according to the ratio of 1:8, soak at room temperature for 10h, add to the high-pressure tank, cover and seal, heat until the reaction temperature reaches Start timing at 170°C, keep the temperature at around 170°C for 30 minutes, and immediately open the ball valve to release the material into the collection tank. Suction filtration is performed when the temperature of the collected material drops to 60-70°C, and the obtained filtrate is corncob steam explosion liquid. After the corncob steam explosion liquid was cooled to room temperature, the total sugar content was determined by the orcinol-hydrochloric acid method, and the direct reducing sugar content was determined by the Somogyi method. The results showed that ...

Embodiment 2

[0058] Embodiment 2, using corn cob steam explosion liquid as raw material to prepare xylose

[0059] 1. Preparation of corn cob steam explosion liquid

[0060] For the specific preparation method, refer to Step 2 of Example 1.

[0061] 2. Preparation of immobilized xylanase-xylosidase

[0062] (1) Preparation of enzyme solution to be immobilized

[0063] Paecilomyes thermophila (Paecilomyes thermophila) J18 seeds were streaked and inoculated from the slant of preserved PDA on a newly prepared PDA plate, and then the plate was placed in a 40-60°C incubator for static culture for 2-8 days.

[0064] Liquid fermentation medium: corn cob powder carbon source 40g / L, tryptone 10g / L, CaCl 2 0.3g / L, MgSO 4 ·7H 2 O 0.3g / L, FeSO 4 0.3g / L; Sterilize at 121°C for 20min, and the pH of the medium is 7.

[0065] Liquid fermentation: Scrape 1 cm from the above Paecilomyces thermophila J18 plate 2 The mycelia of Paecilomyces thermophilia were inoculated in a conical flask containing 60...

Embodiment 3

[0085] Embodiment 3, prepare xylose with birch xylan solution as raw material

[0086] 1. Preparation of immobilized xylanase-xylosidase

[0087] Concrete preparation method is with embodiment 2. The following experiments were all carried out using immobilized xylanase-xylosidase with xylanase activity of 53.9 U / g and xylosidase activity of 34.5 U / g.

[0088] 2. Preparation of xylose by hydrolyzing birch xylan solution with immobilized xylanase-xylosidase

[0089] Inner diameter is the stainless steel jacketed chromatographic column of 12mm, add the xylanase enzyme activity of 40g above-mentioned step 1 preparations and be 53.9U / g, the enzyme activity of xylosidase is the immobilized xylanase of 34.5U / g - Xylosidase to a bed height of 30 cm. Connect the peristaltic pump and the partial collector, equilibrate with 50mM citric acid buffer solution with pH 6.0 for 30min, and set the temperature of the reaction column bed at 60°C. Pump 400mL of 2% (mass percentage) birch xylan...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing xylo-oligosaccharide and xylose by using immobilized enzyme, which comprises the following steps: (1) soaking corn cobs in water, then carrying out steamexplosion on the corn cobs, obtaining the steam explosion liquid; and adjusting the pH value of the steam explosion liquid to be 6.0-7.0; (2) enabling immobilized xylanase and the steam explosion liquid obtained in the step (1) to react under the condition of 50-80DEG C to obtain the xylo-oligosaccharide; and enabling immobilized xylanase-xylosidase and the steam explosion liquid obtained in thestep (1) or birchwood xylan liquid to react under the condition of 50-70DEG C to obtain the xylose. The method for producing the xylo-oligosaccharide and the xylose has the advantages of simple operation, mild reaction condition, good economical efficiency, significant improvement of the utilization ratios of the xylanase and the xylosidase, lowered production cost, and the like.

Description

technical field [0001] The invention relates to a method for producing xylooligosaccharides and xylose by using immobilized enzymes. Background technique [0002] Xylooligosaccharides, also known as xylooligosaccharides, refer to oligosaccharides composed of 2-7 xyloses linked by β-1,4-glycosidic bonds. Because of its unique properties, it has been widely used in the food industry and animal husbandry, and has achieved considerable economic and social benefits in industrial production in recent years. [0003] Xylooligosaccharides are generally obtained by hydrolyzing hemicellulose with xylanase. There are many studies on the hydrolysis of xylan by immobilized xylanase, and the application characteristics are also different. Ai Zhilu et al. (Research on xylanase derived from Streptomyces olivine E-86 immobilized on macroporous resin D380, Food and Fermentation Industry, 2004 (02): 12-24.) selected 14 representative adsorption and The ion exchange resin was tested for the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12N11/04
Inventor 江正强滕超闫巧娟郭洋李里特
Owner CHINA AGRI UNIV