Acetic acid atosiban, and method for detecting content of preparation of acetic acid atosiban and relevant substances
A technology of atosiban acetate and related substances is applied in the field of content determination of atosiban acetate and preparations thereof and related substance determination, can solve problems such as difficult separation completely, and achieves strong specificity, strong operability, Accurate and sensitive effects
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[0034] Example 1
[0035] Determination method of atosiban acetate
[0036] Experimental instrument and chromatographic conditions:
[0037] Waters 515-717-2996 high performance liquid chromatograph, Agilent C18 column (5μm, 150×4.6mm), with 0.05mol / L phosphate buffer (take 6.8g potassium dihydrogen phosphate, add 1000ml of water, adjust with phosphoric acid pH value to 2.0~3.0, then add 120μl of triethylamine to obtain) mobile phase A, acetonitrile as mobile phase B, gradient elution, detection wavelength 205~225nm; flow rate 0.8~1.2ml / min; The sample volume is 5 ~ 200μl. .
[0038] Reagents:
[0039] Acetonitrile (HPLC grade) comes from America Tiandi Company, and the ultrapure water is made by Millipore ultrapure water machine.
[0040] Selection of chromatographic conditions
[0041] 1.1 Mobile phase
[0042] We compared 0.1% trifluoroacetic acid (TFA): acetonitrile (ACN) and 0.05mol / L phosphate buffer (PBS): ACN through experiments. Using 0.1% trifluoroacetic acid aqueous solution ...
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