Method for supplementing material in the fermentation process of transglutaminase

A technology for transglutaminase and fermentation process, which is applied in the field of feeding materials to improve dissolved oxygen in the fermentation process of transglutaminase, and can solve the problem of low dissolved oxygen

Active Publication Date: 2010-04-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low dissolved oxygen in the fermentation process of transglutaminase, the technical problem to be solved by the present invention is to provide a method for supplementing materials in the fermentation process of transglutaminase, which improves the biomass and Production of transglutaminase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: The feeding method in which the ratio of carbon to nitrogen in the nutrient solution is 1:1.0.

[0021] The concentration of glucose in the added nutrient solution is 800g / L, and the concentration of peptone is 800g / L.

[0022] 8h-16h, the control specific growth rate is 0.15h -1 , 16h-24h, the control specific growth rate is 0.10h -1 . This method greatly improves the dissolved oxygen level in the fermentation broth. The dissolved oxygen drops to 10% in 20 hours, and is above 10% in the rest of the time. The viscosity of the fermentation broth is significantly reduced. Reached 6.74U / mL.

Embodiment 2

[0023] Embodiment 2: The feeding method with a carbon-nitrogen ratio of 1:1.5 in the nutrient solution.

[0024] The glucose concentration of the added nutrient solution is 800g / L, and the peptone concentration is 1200g / L.

[0025] 8h-16h, the control specific growth rate is 0.15h -1 , 16h-24h, the control specific growth rate is 0.10h -1 . This method greatly improves the dissolved oxygen level in the fermentation broth. The dissolved oxygen drops to 5% in 20 hours, and is above 5% in the rest of the time. The viscosity of the fermentation broth is significantly reduced. Reached 6.57U / mL.

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Abstract

The invention provides a method for supplementing material in the fermentation process of transglutaminase, in particular to a method for supplementing dissolved oxygen in the fermentation process of transglutaminase, comprising the steps of fermenting Streptomyces hygroscopicus CCTCC M203062 for 8-16 hours; adding a mixed solution of 800 g/L of glucose and 800-1200 g/L of peptone having a carbon-nitrogen ratio of 1.0-1.5 to the fermentation system through flowing so as to control the specific growth rate to be 0.15 1/h and 16-24 h; then adding the mixed solution through flowing to control the specific growth rate to be 0.10 1/h. The method, on the one hand, avoids the nitrogen source being cross-linked by transglutaminase to affect the oxygen dissolution in the fermentation process, and on the other hand, increases the biomass of the bacteria through addition of nitrogen source by means of flowing. The method has obvious effect so that the biomass is increased by 125-140% and the enzyme activity is increased by 58-63%.

Description

technical field [0001] The invention relates to a feeding method in the fermentation process, in particular to a feeding method for increasing dissolved oxygen in the fermentation process of transglutaminase. Background technique [0002] The biological function of microbial transglutaminase (protein-glutamic acid-glutaminase, Microbial Transglutaminase, EC2.3.2.13 referred to as MTG) is to directly change the protein itself and the cells and tissues to which the protein is attached. Characterized by structural and functional properties, enhancing the nutritional value of proteins. Therefore, MTG has broad application prospects in food, textile, biopharmaceutical and other fields. In the previous research, our laboratory screened out a new transglutaminase-producing strain (Streptomyces hygroscopicus CCTCC M203062), optimized its fermentation conditions and explored the activation mechanism of MTG from zymogen to mature enzyme . The previous research results of the labora...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12R1/55
Inventor 陈坚堵国成陈康康张东旭李江华
Owner JIANGNAN UNIV
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