Use of human interleukin 32 in preparation of medicament for treating or preventing influenza A virus infection

Abstract

The invention discloses the use of human interleukin 32 in the preparation of medicaments for treating or preventing influenza A virus infection. Clones of splicing isoforms IL-32(alpha, beta, gamma, delta, epsilon and zeta) of IL-32 are cloned and established by using a molecular biological method; MDCK cells are transfected with the clones; and thus, the applicant has found that MDCK cells in which all subtypes of the human interleukin 32 are overexpressed are improved in resistance to influenza A viruses. Different IL-32 subtypes show different inhibition effects on the replication of the influenza A viruses. The IL-32 gamma has the most obvious anti-influenza A virus effect. And only the IL-32 gamma is of a secretor type. The human interleukin 32 is a critical antiviral gene in immunoreactions of a host. The applicant is the first one to put forward that the human interleukin 32 has an extremely effective anti-influenza A virus function and to point out that the human interleukin 32 has an application prospect in developing anti-influenza A virus medicaments for effectively preventing and treating influenza A virus infection.

Description

technical field [0001] The invention relates to the technical fields of molecular cloning, type A influenza virus titer and virus titer detection, and more specifically relates to an application of human interleukin-32 in a medicine for treating or preventing type A influenza virus infection. Background technique [0002] Typical influenza virus particles of type A are spherical, with a diameter of 80-120 nm, with an average of 100 nm. Influenza A viruses isolated from poultry, some strains are mainly spherical, while some strains are pleomorphic, some are filamentous, and some are odd-shaped particles. The pleomorphism of influenza A virus can be used as a genetic marker in the study of viral genetics, but the polymorphic virus particles initially isolated mainly become spherical after continuous passage in chicken embryos or cell cultures. The envelope of influenza A virus consists of fibrils, a lipid bilayer and matrix proteins. The fibrils on the surface of the capsule...

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Problems solved by technology

[0005] IL-32 can induce the production of IFN-γ in various human and other mammalian cell lines to stimulate human epithelial Wish cells, induce the production of IL-32 in a time-dependent manner in the cell lysate, and reduce it after 46h; IL-18 Stimulation of A549-Rβ (human lung cancer A549 cell line transfected with IL-18Rβ) with IL-1β also induced IL-32 production in a time-dependent manner in its lysate, but not in unstimulated A549-Rβ Synthesis of IL-32 was detected, and only IL-1β could induce IL-32 production in A549-WT (human lung cancer A549 cell line not transfected with IL-18Rβ), similar to the induction of IL-1β in this cell line IL-6 and IL-8, and IFN-γ can induce A549-WT lysate to produce IL-32; the combination of IL-12 and IL-18 can stimulate human NK cells to produce IL-32, and the two cytokines show synergistic effect ; Stimulate human peripheral blood mononuclear cells (PBMC) with mitogen ConA, IL-32 can be detected in its supernatant and lysate after 60h, and the lysate contains more IL-32 than the supernatant, but IL-32 production cannot be detected in human PBMCs stimulated with LPS

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