Micro-operation device for automatically screening Caenorhabditis elegans

Abstract

The invention discloses a micro-operation device for automatically screening Caenorhabditis elegans. The micro-operation device comprises a platform base, a three-axis automatic platform, an optical vision system, an automatic focusing device, an operation arm fine-adjusting device, and an automatic telescopic device of an end manipulator; the three-axis automatic platform, the optical vision system and the operation arm fine-adjusting device are fixedly installed at corresponding positions on the platform base; the automatic focusing device is fixedly arranged on the optical vision system; and the automatic telescopic device of the end manipulator is fixedly arranged on the operation arm fine-adjusting device. The micro-operation device is mainly used for the micro-operations such as screening, sorting and the like to the microorganism such as Caenorhabditis elegans and the like; a control mode of micro-vision servo can realize automatic focusing of the target microorganism and achieve the object of micro-operation by utilizing the end manipulator; and in the invention, all motions are realized automatically under the control of a control system of an industrial control machine, and the whole device has compact structure and convenient operation.

Description

technical field [0001] The invention relates to a micro-operation device for automatic screening of Caenorhabditis elegans, which belongs to the field of biomedicine. Background technique [0002] Caenorhabditis elegans is a classic model organism, because of its transparent body, it is easier to observe; in addition, as the only organism in which all cells in the body can be counted one by one and classified into their own categories, it has been recognized since 1965. Widely used as a research object in life sciences. [0003] The research on Caenorhabditis elegans has achieved remarkable results, such as revealing the phenomenon and mechanism of cell apoptosis, and discovering the phenomenon of RNA silencing and other major life science topics; Such as the treatment of human chronic diseases has pointed out a new way. At the same time, the life science research based on Caenorhabditis elegans is still in progress, and it will explore the irreplaceable material basis of ...

AI Technical Summary

Problems solved by technology

[0004] Due to the small size and short life cycle of Caenorhabditis elegans (only 3 days), it is necessary to continuously observe and track the development process of the nematode during biological research, and to sort and classify the larvae, adults, and corpses of the nematode; currently many Many scientific research institutions use manpower to implement manual sorting methods, which has a large workload and low sorting efficiency; while the development of automatic sorting systems for nematode research in China is rarely mentioned by researchers, especially solid culture. The nematode sorting system in the environment has not yet been developed; most of the foreign high-throughput nematode sorting equipment is based on the flow sorting method in the liquid culture environment, which is not convenient for the life science experiment research of a single nematode, such as When a specific nematode needs to be sorted out for gene injection experiments, it is still necessary to manually participate in manipulating the terminal micromanipulator to perform specific operations

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