Capsule type microbial fertilizer and preparation method thereof

A microbial fertilizer and microbial technology, applied in the field of microbial engineering and plant nutrition, can solve the problems of various factors, short shelf life of effective bacteria, unstable products, etc., so as to prolong the survival period, improve the lasting effect, and enhance the stability. sexual effect

Inactive Publication Date: 2012-10-17
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a capsule-type microbial fertilizer and its preparation method in order to solve the problems of short shelf life of effective bacteria, unstable products, and easy to be affected by various factors in the application process in the existing microbial fertilizers.

Method used

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  • Capsule type microbial fertilizer and preparation method thereof
  • Capsule type microbial fertilizer and preparation method thereof
  • Capsule type microbial fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Sodium alginate concentration (W / V) 2.5%

[0035] Sodium alginate / sodium carboxymethyl cellulose (W / W) 5:1

[0036] Ratio of sodium alginate to bentonite (W / W) 5:1

[0037]Calcium chloride concentration (W / V) 5%

[0038] Mushroom slime / wall material (V / V) 1:1

[0039] Sodium alginate, sodium carboxymethyl cellulose, and bentonite were mixed according to the above proportions, and a calcium chloride solution was prepared; sterilized by damp heat at 121° C. for 20 minutes, and set aside. Mix the pre-cultivated beneficial microorganisms centrifuged at 10,000rpm with the wall material solution in proportion, then use a syringe to draw the mixed solution and drop it into the calcium chloride solution at a uniform speed, fix it for 30 minutes, and then use a non-toxic Rinse the capsules with bacterial water for 3 times to obtain capsule-type microbial fertilizers.

[0040] The particle diameters of the obtained wet and dry capsules are respectively 2.65-2.90mm and 1.00-1....

Embodiment 2

[0042] Sodium alginate concentration (W / V) 2.0%

[0043] Sodium alginate / sodium carboxymethyl cellulose (W / W) 10:1

[0044] Ratio of sodium alginate to bentonite (W / W) 10:1

[0045] Calcium chloride concentration (W / V) 5%

[0046] Mushroom slime / wall material (V / V) 3:1

[0047] The preparation method is the same as in Example 1.

[0048] The properties of the obtained capsules are: the particle diameters of the wet and dry capsules are 2.48-2.70mm and 0.90-1.10mm respectively, the water content is 95%, the diameter diffusion coefficient of the dry capsule is 1.42, the mass diffusion coefficient is 2.0, and the embedding rate is 99.99% %, containing up to 10 live bacteria 14 CFU / g, the number of live bacteria that can be released is up to 10 9-10 CFU / g. Dry to constant weight at room temperature. After two months of storage, the effective bacteria content can reach 10 8-9 CFU / g, the obtained capsules are as attached figure 1 , 2 , 3, 4 shown.

Embodiment 3

[0050] Sodium alginate concentration (W / V) 1.5%

[0051] Sodium alginate / sodium carboxymethyl cellulose (W / W) 8:1

[0052] Ratio of sodium alginate to bentonite (W / W) 8:1

[0053] Calcium chloride concentration (W / V) 2%

[0054] Mushroom slime / wall material (V / V) 2:1

[0055] The preparation method is the same as in Example 1.

[0056] The particle diameters of the obtained wet and dry capsules are respectively 2.38-2.55mm and 0.85-1.00mm, the water content is 96%, the diameter diffusion coefficient of the dry capsule is 1.40, the mass diffusion coefficient is 1.9, and the embedding rate is 99.99%. Viable bacteria count up to 10 14 CFU / g, the number of live bacteria that can be released is up to 10 9-10 CFU / g. Dry to constant weight at room temperature. After two months of storage, the effective bacteria content can reach 10 8-9 CFU / g.

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Abstract

The invention relates to a capsule type microbial fertilizer and a preparation method thereof, and belongs to the technical fields of microbiology engineering and plant nutrition. In the capsule type microbial fertilizer, environmentally-friendly and biodegradable substances are taken as a wall material, and useful microorganisms are taken as a core material and are capsulated by a piercing-solidifying incuber method; the environment-friendly and biodegradable wall material is calcium alginate, sodium carboxymethylcellulose and bentonite; and the useful microorganisms are Raoultella planticola, Klebsiella oxytoca, Enterobacter cloacae and Bacillus subtilis. The grain sizes of wet and dry capsules are respectively between 2.40 and 3.50mm and between 0.8 and 1.5mm; the water content is 90 to 98 percent; the diameter diffusion coefficient of dry capsules is 1.3 to 1.5; the quality diffusion coefficient is 1.9 to 2.5; the embedding rate is over 99 percent; the viable count is 1,014CFU/g; and the releasable viable count is 109 to 10CFU/g. After the capsule type microbial fertilizer is stored for 2 months, the effective bacteria amount can reach 108-9CFU/g. The microbial fertilizer has the advantages of long shelf life and stable product, overcomes influence of various factors in the application process, and has the effects of preventing diseases, resisting diseases, relieving salt stress, promoting plant growth and the like in the seedling stage of crops.

Description

technical field [0001] The invention relates to the development of a capsule-type microbial fertilizer and a preparation method thereof, belonging to the technical field of microbial engineering and plant nutrition. The development of the microbial fertilizer dosage form can solve the problems of short shelf life of effective bacteria in microbial fertilizers, unstable products, and easy to be affected by various factors in the application process, and can effectively improve the survival period, persistence and stability of bacteria and environmental adaptability. Background technique [0002] At present, the fertilizers used in agricultural production are mainly chemical fertilizers. However, due to their low utilization rate and serious residues, their long-term and large-scale use has caused adverse consequences such as soil compaction, organic matter decline, and soil nutrient ratio imbalance. Therefore, there is an urgent need for a better product to replace chemical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00
Inventor 李春赵雅峰武占省
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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