Method for preparing genetic material born by microvia stent

A micro-blind hole and gene technology, applied in the fields of biomedical materials and biochemistry, can solve the problems of poor repeat accuracy, inability to scale processing, and inability to accurately control the amount of genetic material carried, achieving high automation and good repeatability Effect

Inactive Publication Date: 2010-06-09
董何彦
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has many disadvantages, such as the solution cannot fully and effectively enter the micro-blind holes of the stent; the amount of genetic material carried on the surface of a single stent cannot be precisely controlled; the repeatability of the operation process is poor, and large-scale processing cannot be performed.

Method used

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  • Method for preparing genetic material born by microvia stent
  • Method for preparing genetic material born by microvia stent
  • Method for preparing genetic material born by microvia stent

Examples

Experimental program
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Embodiment Construction

[0023] 1. Single layer spraying method

[0024] Prepare 2mg / ml naked plasmid DNA with ultrapure water, add sucrose after fully dissolving, so that the sucrose concentration in the solution reaches 1.1%; put the prepared solution into the coating solution pool 8, and connect the nozzle joint with a tetrafluoroethylene hose 2 and the joint 7 of the coating solution pool; the micro-blind hole support 4 is installed on the support fixture 5; start the spraying system, the power of the ultrasonic generator 1, the propulsion speed of the support propeller 6, and the propulsion of the coating solution propeller 9 The speed is set so that the micro-blind vascular hole support 4 can reach the expected genetic material load once it is reciprocated from the nozzle 3 .

[0025] 2. Multi-layer spraying method

[0026] Prepare a 2mg / ml genetic material solution with ultrapure water, add sucrose after fully dissolving, so that the sucrose concentration in the solution reaches 1.1%; put the ...

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Abstract

The invention relates to a method for preparing a genetic material born by a microvia stent. The method is characterized in that the born genetic material is dissolved at normal temperature, and a proper amount of gene activity protective agent is added; ultraphonic micro nebulization pressurizing spraying equipment is used to spray to enable the genetic material to enter the inside of a microvia and be coated on the surface of the stent. The invention adopts the microvia stent to bear the genetic material, enables the genetic material to play a role of rapid endothelialization of the genetic material in clinical applications so as to improve therapeutic effect. Firstly, the born genetic material is dissolved at normal temperature, and the proper amount of gene activity protective agent is added to ensure the genetic material to normally take effects; and secondly, by adopting the ultraphonic micro nebulization pressurizing spraying method, the genetic material is enable to successfully attach to the surface of the microvia stent, and the carrying capacity of the genetic material on the surface of each stent controllable is controllable with high repeatability precision.

Description

technical field [0001] The invention relates to a medical device and a preparation method for carrying genetic material thereof, and belongs to the fields of biomedical materials, biochemistry, and the like. In addition to the preparation of naked plasmids coated with genetic material on the surface of micro-blind hole stents (for the preparation method, see "Method for making drug-loaded layer of micro-blind holes on the surface of metal stents" patent number: ZL200610109422.8;), it also includes gene A method for dissolving and protecting substances, especially a method for preparing naked plasmids carrying gene substances on a micro-blind hole scaffold. Background technique [0002] Interventional angioplasty of cardiovascular stents in medical devices has become a more and more widely used method in the current medical field. However, simple cardiovascular stent implantation is facing a serious challenge, that is, the problem of biocompatibility and tissue regeneration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/56A61L27/06A61L27/04A61L27/14A61F2/82
Inventor 董何彦李昕跃李相宜
Owner 董何彦
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