Method for preparing magnetic silica microspheres with surfaces modified by cations
A technology of silica and surface modification, which is applied in the preparation of silica, silica, and test samples, etc., can solve the problems of nucleic acid loss, time-consuming and labor-intensive nucleic acid purification process, etc., and achieve large amount of nucleic acid, simple operation, and design reasonable effect
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Embodiment 1
[0050] Example 1 Preparation of magnetic silica microspheres with surface-modified histidine
[0051] 1. Preparation of Magnetic Silica Microspheres
[0052] Take 7.2892g of cetyltrimethylammonium bromide and 109.3g of toluene into the four-necked flask, stir evenly with a stirrer at 1000 rpm, mix 0.329g of FeCl 2 4H 2 O with 0.987g FeCl 3 ·6H 2 Dissolve O in 9.4397g of secondary water to prepare an aqueous solution with a concentration of 140g / L, add dropwise to the above mixture at 4-5mL / h under nitrogen protection at 2-3mL / min, and continue stirring at 800 rpm for 4-6 hours , then add 2 mL of ammonia water with a mass concentration of 25%, and continue to stir at 800 rpm for 2 hours. In the mixture, iron ferric oxide nanoparticles are formed, slowly add 9.79 mL of tetraethyl orthosilicate, and stir at 800 rpm. Adjust the pH to 8, 1 hour after adding tetraethyl orthosilicate dropwise at 2-3mL / h, stop the nitrogen protection, stir at 800 rpm, age for 5 days at normal temp...
Embodiment 2
[0057] Example 2 Preparation of magnetic silica microspheres with surface-modified polyhistidine
[0058] 1. Preparation of Magnetic Silica Microspheres
[0059] Take 7.2892g of cetyltrimethylammonium bromide and 109.3g of toluene into the four-necked flask, stir evenly with a stirrer at 1000 rpm, mix 0.329g of FeCl 2 4H 2 O with 0.987g FeCl 3 ·6H 2 Dissolve O in 9.4397g of secondary water to prepare an aqueous solution with a concentration of 140g / L, add dropwise to the above mixture at 4-5mL / h under nitrogen protection at 2-3mL / min, and continue stirring at 800 rpm for 4-6 hours , then add 2 mL of ammonia water with a mass concentration of 25%, and continue to stir at 800 rpm for 2 hours. In the mixture, iron ferric oxide nanoparticles are formed, slowly add 9.79 mL of tetraethyl orthosilicate, and stir at 800 rpm. Adjust the pH to 8, 1 hour after adding tetraethyl orthosilicate dropwise at 2-3mL / h, stop the nitrogen protection, stir at 800 rpm, age for 5 days at normal ...
Embodiment 3
[0064] Embodiment 3 prepares the magnetic silica microsphere of surface modification imidazole
[0065] 1. Preparation of Magnetic Silica Microspheres
[0066] Take 7.2892g of cetyltrimethylammonium bromide and 109.3g of toluene into the four-necked flask, stir evenly with a stirrer at 1000 rpm, mix 0.329g of FeCl 2 4H 2 O with 0.987g FeCl 3 ·6H 2 Dissolve O in 9.4397g of secondary water to prepare an aqueous solution with a concentration of 140g / L, add dropwise to the above mixture at 4-5mL / h under nitrogen protection at 2-3mL / min, and continue stirring at 800 rpm for 4-6 hours , then add 2 mL of ammonia water with a mass concentration of 25%, and continue to stir at 800 rpm for 2 hours. In the mixture, iron ferric oxide nanoparticles are formed, slowly add 9.79 mL of tetraethyl orthosilicate, and stir at 800 rpm. Adjust the pH to 8, 1 hour after adding tetraethyl orthosilicate dropwise at 2-3mL / h, stop the nitrogen protection, stir at 800 rpm, age for 5 days at normal te...
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