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Reagent used for diagnosing helicobacter pylori infection and preparation method thereof and kit thereof

A Helicobacter pylori and reagent technology, which is applied in the field of reagents for diagnosing Helicobacter pylori infection, can solve problems such as large errors, inconvenient use, and many operating steps, and achieve the effects of reduced production costs, convenient use, and reduced false negatives

Inactive Publication Date: 2010-06-09
SHENZHEN ZHONGHE HEADWAY BIO SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The technical problem to be solved by the present invention is to provide a solution with few operating steps and convenient for the defects of the prior art, which is inconvenient to use, requires doctors to carry out more solution preparation and transfer work, and has many operating steps, which may easily lead to accidents or large errors. Reagents used by doctors to diagnose Helicobacter pylori infection

Method used

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  • Reagent used for diagnosing helicobacter pylori infection and preparation method thereof and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Weigh 0.72g 2,5-diphenyloxazole (hereinafter referred to as PPO), 0.036g 1,4-bis(2-methylstyryl)benzene (hereinafter referred to as bis-MSB), and measure 50ml linear alkane Put the base benzene together in a 200ml beaker, place it on a constant temperature magnetic stirrer and dissolve it under heating and stirring. After cooling to room temperature, add 20ml of polysorbate emulsifier and 4ml of fatty alcohol polyoxyethylene ether emulsifier, and then stir evenly to obtain a scintillation solvent . Subsequently, add 0.035g of phenolphthalein indicator into 10ml of ethanolamine, stir well and set aside.

[0043] The reagent for diagnosing Helicobacter pylori infection of the present invention can be obtained by dispensing 0.4ml of ethanolamine and 3.5ml of scintillation solvent per scintillation vial. When in use, the patient blows air into the scintillation vial with an air blowing catheter, and stops blowing when the solution changes from red to milky white, shakes it...

Embodiment 2

[0047] Weigh 0.24g of PPO, 0.012g of bis-MSB, measure 40ml of linear alkylbenzene and put them into a 200ml beaker together, place them on a constant temperature magnetic stirrer and heat and stir to dissolve them. After cooling to room temperature, add 14ml of octyl polyoxyethylene Ether (also known as Triton X-100) was then stirred evenly to obtain a scintillation solvent.

[0048] Subsequently, 0.45 ml of ethanolamine and 2.7 ml of scintillation solvent were dispensed per scintillation vial. When in use, the patient blows air into the scintillation vial for more than 3 minutes with an air blowing tube. After stopping the air blowing, shake it gently and put it directly into the scintillation measuring instrument for measurement. According to the measurement results, it is diagnosed whether the patient is infected with Helicobacter pylori.

[0049] In this embodiment, each bottle of the reagent for diagnosing Helicobacter pylori infection contains about 0.45ml of ethanolamin...

Embodiment 3

[0051] Weigh 0.25g of PPO, 0.032g of 1,4-bis(5-phenyl-2-oxazolyl)benzene (referred to as POPOP), measure 40ml of linear alkylbenzene and put them together in a 200ml beaker, and place them under constant temperature magnetic stirring Dissolve under heating and stirring on a container, after cooling to room temperature, add 20ml of nonyl polyoxyethylene ether emulsifier, and then stir evenly to obtain a scintillation solvent.

[0052] Subsequently, 0.45ml of ethanolamine and 3.0ml of scintillation solvent were dispensed per scintillation vial. When in use, the patient blows air into the scintillation vial for more than 3 minutes with an air blowing tube. After stopping the air blowing, shake it gently and put it directly into the scintillation measuring instrument for measurement. According to the measurement results, it is diagnosed whether the patient is infected with Helicobacter pylori.

[0053] In this embodiment, each bottle of the reagent for diagnosing Helicobacter pylo...

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Abstract

The invention relates to reagent used for diagnosing helicobacter pylori infection and preparation method thereof and kit thereof; wherein the reagent contains 0.3-0.5ml of CO2 absorbent, 2-3ml of organic solvent, 0.7-2ml of emulsifier and 0.01-0.038g of scintillator. The reagent used for diagnosing helicobacter pylori infection, preparation method thereof and kit thereof are applied, CO2 absorption and scintillation measurement on all the required substances are merged, thus reducing operation step and being more convenient for doctor to use. Further more, under the same experiment condition, DPM value can be above 30% higher than that of the existing assay kit; the reagent has positive effect for judging weak positive close to critical value appeared in clinic, sensitivity of measurement method can be improved, and false negative is reduced; usage amount of reagent is reduced by about 30%, thus being beneficial to reducing production cost and reducing adverse effect on environment; and use is safer.

Description

technical field [0001] The present invention relates to medicinal chemicals, and more particularly to the use of 14 C-UBT (Urea BreathTest, urea breath test) is used to diagnose the reagent of Helicobacter pylori (Helicobacter pylori, Hp) infection, its preparation method and the test kit using this reagent. Background technique [0002] Helicobacter pylori was first isolated from a gastric mucosal biopsy specimen of a patient with chronic active gastritis by Australian scholars Robin Warren and Barry J. 2005 "Nobel Prize in Physiology or Medicine". [0003] Human beings are the only natural host of Helicobacter pylori, and the population infection rate is very high, as high as 30-50% in the whole world. Helicobacter pylori can cause many diseases, so its clinical diagnosis and treatment are very important. [0004] Urea[ 14 C] The breath test is currently the most widely used diagnostic method for Helicobacter pylori at home and abroad. Due to its painless, non-invasive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/01
Inventor 沈桂富郭春生黄晋杰
Owner SHENZHEN ZHONGHE HEADWAY BIO SCI & TECH CO LTD
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