Protein chip for rapidly detecting pectoral ascites cancer cells and preparation method thereof

Abstract

The invention relates to a protein chip for rapidly detecting pectoral ascites cancer cells and a preparation method thereof. The chip is provided with a solid matrix glass slide; and anti-tumor antigenic specific antibodies marked by various types of colloidal gold are combined and distributed at different positions on the surface of the glass slide in a dot matrix way. The preparation method comprises the following steps of: preparing the colloidal gold, the antibodies marked by the colloidal gold by using a trisodium citric acid reduction method; performing sample application on a prepared aldehyde group glass slide and the like. In a clinical practice, the cancer cells in pectoral ascites can be combined with the corresponding specific antibodies in the chip after pectoral ascites cast-off cell suspension and the chip are incubated together, and the combined cancer cells can be observed by a microscope so as to achieve the purpose of rapidly detecting and diagnosing cancerous pectoral ascites. The product has the characteristics of firm protein combination, good stability, difficult degradation, suitability for transportation and storage at room temperature, high throughput, time saving, low price and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a protein chip and a preparation method thereof, in particular to a protein chip for rapid detection of pleural effusion cancer cells and a preparation method thereof. Background technique [0002] Pathological diagnosis of cancer cells in pleural and ascites has long been a difficult problem for pathologists, especially when the proliferation of mesothelial cells in pleural and ascites is obvious, they are often very similar in shape to well-differentiated cancer cells, which can be easily confused and cause pathological problems. Diagnosis has brought great difficulties, and even pathologists with rich clinical experience may misdiagnose, which is also a difficult problem in the current international cytopathology community. [0003] For the identification of mesothelial cells and cancer cells in pleural effusion, the most effective solution in the past is to distinguish meso...

AI Technical Summary

Problems solved by technology

[0004] The application text of the patent No. 03111532.2 once disclosed a biochip technology named "Automatic capture and identification of cell biochip and its preparation method". Although this biochip can detect and automatically capture cancer cells in the pleural effusion, in To a certain extent, it has the function of distinguishing pleural and ascites cancer cells and mesothelial cells, but its chip preparation technology requires high requirements, and the spotting protein on the chip is not stable and easy to degrade, and the transportation and storage conditions are high. Degradation, invalidation, cancer cells in the pleural and ascites can no longer be detected when used, so the application is limited, and it is difficult to promote the use in clinical pathological diagnosis