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A kind of sheep hemoglobin polypeptide powder and preparation method thereof

A technology of hemoglobin and polypeptide powder, which is applied in the field of food nutrition enhancers, functional foods, and functional food raw materials. It can solve the problems of no sheep hemoglobin polypeptide powder, sheep blood with smell, and difficult food ingredients, etc., and the preparation method is simple. Ease of use, high nutritional value and low cost

Inactive Publication Date: 2012-02-15
辽源市麒鸣生物技术综合开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the patents related to hemoglobin products mainly include "A Hemoglobin Polypeptide Powder and Its Preparation Method" (Application No.: 200810222237.9), "A Hemoglobin Polypeptide Amino Acid Nutrient and Its Preparation Method" (Application No.: 200810222240.0), "A An Antioxidant Active Peptide and Its Preparation Method" (Application No.: 200810103445.7), but no report about sheep hemoglobin polypeptide powder was found. One of the reasons is that sheep blood has a smell and is difficult to use as a food ingredient.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 sheep hemoglobin polypeptide powder

[0030] Follow the steps below:

[0031] (1), adding 10 g of trisodium citrate solution with a concentration of 20% to 500 g of sheep blood, stirring evenly, then centrifuging at 5000 g (TGL-16A centrifuge, Changsha Pingfan Instrument Co., Ltd.) for 6 min, separating to obtain two-layer solution , the lower floor is a sheep hemoglobin solution (325g), and the upper floor is a sheep plasma protein solution, and the sheep hemoglobin solution (325g) of the lower floor is collected.

[0032] (2), the goat hemoglobin solution is placed in an airtight container, then evacuated with a vacuum pump, and when the vacuum reaches 0.085 MPa, the temperature is raised to 65° C. and the goat hemoglobin solution is pumped for 3 minutes to obtain a goat hemoglobin solution without mutton.

[0033] (3), dilute the sheep hemoglobin solution obtained in step (2) with water to a concentration of 12%, adjust the pH to 6.5,...

Embodiment 2

[0035] The preparation of embodiment 2 sheep hemoglobin polypeptide powder

[0036] Follow the steps below:

[0037] (1), adding 22 g of 20% trisodium citrate solution to 1000 g of sheep blood, stirring evenly, then centrifuging at 5500 g (TGL-16A centrifuge, Changsha Pingfan Instrument Co., Ltd.) for 6 min, separated to obtain two layers of solution , the lower floor is sheep hemoglobin solution, the upper floor is sheep plasma protein solution, and the sheep hemoglobin solution (655g) of the lower floor is collected.

[0038] (2), the goat hemoglobin solution is placed in a closed container, and then evacuated with a vacuum pump. When the vacuum reaches 0.086 MPa, the temperature is raised to 65° C. and the goat hemoglobin solution is pumped for 4 minutes to obtain the goat hemoglobin solution without mutton.

[0039] (3), dilute the sheep hemoglobin solution obtained in step (2) with water to a concentration of 13%, adjust the pH to 6.5, add 0.9 g of papain, and carry out ...

Embodiment 3

[0041] The preparation of embodiment 3 sheep hemoglobin polypeptide powder

[0042] Follow the steps below:

[0043](1), adding 18 g of 20% trisodium citrate solution to 1000 g of sheep blood, stirring evenly, then centrifuging at 5000 g (TGL-16A centrifuge, Changsha Pingfan Instrument Co., Ltd.) for 8 minutes, separated to obtain two layers solution, the lower floor is a sheep hemoglobin solution, and the upper floor is a sheep plasma protein solution, and the sheep hemoglobin solution (648g) of the lower floor is collected.

[0044] (2), gained goat hemoglobin solution is placed in airtight container, then with vacuum pump pumping, when vacuum degree reaches 0.087 MPa, be warming up to 64 ℃ of pumping for 4 minutes, obtain the goat hemoglobin solution without mutton.

[0045] (3), dilute the sheep hemoglobin solution obtained in step (2) with water to a concentration of 13%, adjust the pH to 6.6, add 1.0 g of papain, and carry out the hydrolysis reaction at 37 ° C and pH 6....

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PUM

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Abstract

The invention discloses sheep hemoglobin polypeptide powder and a preparation method thereof, belonging to the field of food additives. The present invention mainly adds trisodium citrate to sheep blood, then centrifuges to obtain sheep hemoglobin solution, then puts sheep hemoglobin solution in a closed container and uses a vacuum pump to remove the smell of mutton; then dilutes sheep hemoglobin solution with water , add papain to carry out enzymolysis reaction, centrifuge to collect the enzymolysis product; then use ultrafiltration membrane to ultrafilter, concentrate and dry to obtain sheep hemoglobin polypeptide powder. The sheep hemoglobin polypeptide powder of the present invention has high nutritional value, and has the functions of anti-oxidation, immune regulation, and blood pressure lowering; secondly, the sheep hemoglobin polypeptide powder of the present invention has no smell, and is easy to accept by consumers; the sheep hemoglobin polypeptide powder of the present invention has high safety, no Side effects; the preparation method of the invention is simple and easy, and the cost is low; the invention reduces the discharge of sheep blood and reduces environmental pollution.

Description

technical field [0001] The invention belongs to functional food raw materials, functional food and food nutrition enhancer, in particular to sheep hemoglobin polypeptide powder and a preparation method thereof. Background technique [0002] Blood protein has a very high biological value, contains 8 kinds of essential amino acids needed by the human body, and its composition is balanced, which is a high-quality protein source. However, at present, animal blood, especially sheep blood, is mostly discharged or discarded in the form of sewage, resulting in the loss of a large amount of valuable nutritional resources and causing serious pollution to the environment. In sheep blood, hemoglobin accounts for 60-70% of its total protein content. Hemoglobin bioactive peptides have many extremely important functional activities, such as opioid activity (Brantl, 1986; Piot, 1992; Zhao, 1996; Zhao, 1997; Nyberg, 1997; Lignot, 1999), ACE inhibitory activity (Hyun & Shin, 2000) , enhance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/12A23J1/06A23J3/34
Inventor 苏岩薛可苏东明伏圣兴杨苏扬
Owner 辽源市麒鸣生物技术综合开发有限公司