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Novel chalcone compound and preparation method and application thereof

A technology for chalcone and compound, which is applied in the field of chalcone compound and its preparation, can solve the problems of lack of scientific basis for clinical drug use, complex chemical composition of Pseudomonas falciparum, difficulty in formulating quality standards, etc., and achieves reasonable design of process route. , Single ingredient, strong inhibitory effect

Inactive Publication Date: 2010-06-30
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Clinically, the whole herb is mainly used as medicine, but the chemical composition of Oxytropis falciparum is complex, the substances that play a medicinal role are unclear, it is difficult to formulate its quality standards, and there is no scientific basis for clinical use

Method used

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  • Novel chalcone compound and preparation method and application thereof
  • Novel chalcone compound and preparation method and application thereof
  • Novel chalcone compound and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 new chalcone compound:

[0032] Such as figure 1 Shown, the preparation method of the new chalcone compound provided by the invention comprises the following steps:

[0033] (1) Take 500g of Oxytropis falciparum dried medicinal material, chop, remove residue, soak with 95% ethanol for 3 weeks, and shake every day; after 3 weeks, take out the extract, filter, and the filtrate is decompressed by a rotary evaporator Concentrate to extract, set aside;

[0034] (2) Put the extract obtained in step (1) into a 500ml beaker, add 200ml of petroleum ether (60-90°C) for ultrasonic extraction, replace the petroleum ether every 20min, and the color of petroleum ether becomes light after extraction for 2 hours, discard the petroleum Ether extract, keep the remaining extract; add 200ml of dichloromethane to the remaining extract for ultrasonic extraction, replace the dichloromethane every 20min, after extraction for 3h, combine all the collected dichl...

Embodiment 2

[0042] The preparation of embodiment 2 new chalcone compounds:

[0043] Such as figure 1 Shown, the preparation method of the new chalcone compound provided by the invention comprises the following steps:

[0044] (1) Take 500g of Oxytropis falciparum dried medicinal material, chop, remove residue, soak with 90% ethanol for 4 weeks, and shake every day; after 4 weeks, take out the extract, filter, and the filtrate is decompressed by a rotary evaporator Concentrate to extract, set aside;

[0045] (2) Put the extract obtained in step (1) into a 500ml beaker, add 200ml of petroleum ether (60-90°C) for ultrasonic extraction, replace the petroleum ether every 20min, and the color of petroleum ether becomes light after extraction for 2 hours, discard the petroleum Ether extract, keep the remaining extract; add 200ml of dichloromethane to the remaining extract for ultrasonic extraction, replace the dichloromethane every 20min, after changing 6 times, combine all the collected dichl...

Embodiment 3

[0053] Example 3 Anti-tumor experimental research on new chalcones

[0054] 1. Preparation of the test drug: Take an appropriate amount of the new chalcone compound monomer provided by the invention, dissolve it with dimethyl sulfoxide (DMSO), and prepare the concentration of 1.25 μg, 2.5 μg, 5 μg, and 10 μg respectively. , 20 μg of the test drug, the final concentration of DMSO was 1.0%, after filter sterilization, it was stored at 4°C for later use, and dimethyl sulfoxide was used as the test control group.

[0055] 2. Experimental process

[0056] Human liver cancer cell line SMMC-7721, human cervical cancer cell line Hela, human gastric cancer cell line MGC-803, human colon cancer cell line LOVO, human breast cancer cell line MDA-MB-231, and human lung cancer cell line A549 were selected. Liver cancer cell line SMMC-7721, human breast cancer cell line MDA-MB-231 and human colon cancer cell line LOVO cell line were cultured in RPMI-1640 medium containing 10% calf serum. Hu...

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Abstract

The invention discloses novel a chalcone compound extracted and purified from Oxytropis falcate, a preparation method and an application thereof. Identified through a spectral method, the novel chalcone compound has the structural formula C15H1203 and is named 2', 4'-dihydroxy chalcone, and the preparation method combines column chromatography and preparative liquid purification method. Result from experiments in vitro shows that the novel chalcone compound can remarkably restrain the growth and proliferation of tumor cells and has good efficacy of restraining acute inflammation of mice and chronic inflammation of rats. Therefore, the novel chalcone compound is expected to be developed to be a new generation of antitumor drugs or anti-inflammatory drugs.

Description

technical field [0001] The invention relates to a compound, in particular to a chalcone compound, a preparation method thereof and an application thereof. Background technique [0002] Oxytropis falcatae is the dry whole herb of Oxyptropis falcatae Bunge, a leguminous plant. The Tibetan medicine name is "E Daxia", which is recorded in the 1977 edition of "Chinese Pharmacopoeia". This medicine is widely used clinically by Tibetan medicine. It is the main drug of various Tibetan medicine compound prescriptions such as Liuwei Sickle-shaped Jidou Powder and Qizheng Xiaotong Plaster. It is a national medicine with research and development value. [0003] In recent years, domestic scholars have carried out research on the chemical composition of this medicinal material, and isolated a variety of alkaloids, saponins and flavonoids, such as condylarin, 7-hydroxydihydroflavone, quercetin, stigmasterol, carrot Glycosides and swainsonine etc. In terms of pharmacology, in 1979, Wei Qu...

Claims

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Application Information

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IPC IPC(8): C07C49/835C07C45/78A61K31/12A61P35/00A61P29/00A61P7/04
Inventor 蔡宝昌许惠琴李祥杨欢陈建伟吴诚沈存思崔姣楼成华杨光明
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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