Lactobacillus, paracasei subsp. paracasei strain, bacteriostatic composition and applications thereof

A paracheese and lactobacillus technology, applied in the field of microorganisms, can solve problems such as the decline of the flavor and quality of livestock and poultry products, the health of drug residues, and the health hazards of children

Active Publication Date: 2010-07-07
SYNGEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of adding antibiotics to feed is to promote growth, reduce roughage, and improve the growth efficiency of livestock and poultry. However, due to the shortened growth cycle of livestock and poultry, the precipitation of nutrients will inevit

Method used

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  • Lactobacillus, paracasei subsp. paracasei strain, bacteriostatic composition and applications thereof
  • Lactobacillus, paracasei subsp. paracasei strain, bacteriostatic composition and applications thereof
  • Lactobacillus, paracasei subsp. paracasei strain, bacteriostatic composition and applications thereof

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Embodiment 1

[0039] Embodiment one The screening and separation of Lactobacillus paracasei subspecies SG96 bacterial strain of the present invention

[0040] Pig intestinal excreta were collected and put into MRS broth medium (Difco TM , REF288130), after culturing in an anaerobic environment at 37°C for 24 hours, the culture was spread on MRS agar plates (Difco TM , REF288210), and then cultured at 37°C for 3 days. After culturing, the colonies that appeared on the agar medium were collected, and a total of more than 1,000 strains of acid-producing bacteria were obtained, and then the filter paper agar diffusion method (disc-agar diffusion) was used to screen out Escherichia coli (E.coli) (BCRC11634) and The lactic acid bacteria of Salmonella (S.typhimurium) (BCRC129407), then select the most active functional Lactobacillus paracasei subsp. SG96 (Lactobacillus paracasei subsp.paracasei) SG96. The culture characteristics of this bacterial species are as follows: on MRS agar Gray colonies...

Embodiment 2

[0041] Embodiment two Gram staining of Lactobacillus paracasei subspecies SG96 bacterial strain of the present invention

[0042] The characteristics of the strains of the present invention were detected by Gram staining. The result is as Figure 1A As shown, the strains of the present invention are Gram-positive bacteria, non-sporing anaerobes, and non-mobility bacteria, which are typical characteristics of Lactobacillus.

[0043] The mycological characteristics of Lactobacillus paracasei subspecies SG96 bacterial strain of the present invention are as follows:

[0044] (a) Morphological features:

[0045] (1) Cell shape and size: When the cells are placed in MRS broth medium and cultured in an anaerobic environment at 37°C for 24 hours, rod-shaped bacilli (such as Figure 1B shown).

[0046] (2) Mobility: no movement

[0047] (3) Flagella: None

[0048] (4) Sporulation: no sporulation

[0049] (5) Gram stain: positive

[0050] (b) Culture characteristics:

[0051] (1...

Embodiment 3

[0057] Embodiment three The 16SrDNA sequence analysis of Lactobacillus paracasei subspecies SG96 bacterial strain of the present invention

[0058] 1. DNA extraction

[0059] Using FavorPrep TM Purify DNA with Blood Genomic DNA Extraction Mimi Kit, cultivate the bacteria overnight, take 200 μL bacterial liquid and add 20 μL proteinase K (add 110 μL ddH before using proteinase K 2 O shake and mix for 5 minutes to make the concentration 11 mg / mL, store on ice at -20°C for later use), add 200 μL of FABG buffer, shake and mix for 5 seconds, heat at 60°C for 15 minutes and centrifuge briefly. Add 200 μL of 95% alcohol, shake and mix for 10 seconds, briefly centrifuge, add to the FABG column (FABG column), and put the FABG column into the CollectionTube, and centrifuge at 10,000 rpm for 2 minutes. Take out the FABG column after centrifugation, pour out the supernatant and dry it with toilet paper, put the FABG column back into the Collection Tube, add 500μL of W1 buffer (8mL of 9...

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Abstract

The invention discloses a Lactobacillus, paracasei subsp. paracasei strain, a bacteriostatic composition and applications thereof. The preservation number of the SG96 strain microorganism is CGMCC2697. The strain can inhibit the growth of pathogenic bacteria of Escherichia coli, Salmonella typhimurium and the like, and has bacteria killing effect. The invention also discloses a bacteriostatic composition containing the Lactobacillus, paracasei subsp. paracasei strain SG96. The composition can be prepared into animal water-feeding additives, animal fodder additives, animal and human medical compositions, food additives, beverage additives, foods, beverages, health foods, and the like.

Description

technical field [0001] The present invention relates to a kind of Lactobacillus paracasei subspecies (Lactobacillus paracasei subsp.paracasei) SG96 bacterial strain, relate in particular to a kind of Lactobacillus paracasei subsp. A microbial strain, and a bacteriostatic composition containing the Lactobacillus paracasei subsp. paracasei and uses of the composition belong to the field of microorganisms. Background technique [0002] In recent decades, due to the rapid increase in population and the substantial improvement in material living standards, people’s demand for meat has increased significantly. In order to meet market demand, many economic animal breeding has gradually become large-scale, especially in backward countries. The ability to import food is relatively low. Low, so a large number of high-density or large-scale breeding of economic animals leads to the rapid spread of many diseases among animals. In order to improve the sickness of economical animals or a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/04A61P1/12A23K1/16A23L1/30A23L1/29A23L2/52C12R1/225A23L33/00A61K35/747
CPCY02A50/30
Inventor 魏钰珊戴芳雲
Owner SYNGEN BIOTECH
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