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Method for separating uridylic acid from biocatalytic conversion solution

A technology of biocatalysis and conversion solution, applied in the field of separation of uridine acid, which can solve the problems of long production cycle, high separation difficulty, cumbersome process, etc., and achieve the effect of low scale production cost, simple preparation process and cumbersome extraction process

Inactive Publication Date: 2010-07-21
NANJING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Nucleotide is an important biochemical raw material, which can be used in food additives, pharmaceutical intermediates, feed additives, and plant growth promoters. Uridine acid (UMP) is a basic nucleotide and is a The most important kind of uridine acid, the market demand is very large, but the production of uridine acid is difficult, resulting in high prices. Most of the nucleotide production in my country is produced by enzymatic hydrolysis, which has a long production cycle, cumbersome process, and high difficulty in separation. Lead to high production cost, poor quality, low yield, and the purity cannot meet the production requirements of downstream medicines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] According to the method for preparing biocatalytic conversion liquid, 10 L of conversion liquid was prepared, wherein the content of UMP was 8.4 g / L.

[0035] The treatment process is as follows:

[0036] (1) Adjust the pH value of the biocatalytic conversion solution to 2.0 with hydrochloric acid, and then centrifuge at 10,000 rpm for 20 minutes to remove solid impurities.

[0037] (2) the centrifugal supernatant that step (1) is obtained is processed through ultrafiltration, and the ultrafiltration permeate is collected; The ultrafiltration membrane is a polyamide membrane, and the ultrafiltration membrane molecular weight cut-off is 8000, and the ultrafiltration membrane working pressure is 1MPa, the ultrafiltration temperature is 30°C, and the ultrafiltration pH value is 5.0.

[0038] (3) The ultrafiltration permeate obtained in step (2) is processed by nanofiltration to collect the nanofiltration concentrate; the nanofiltration membrane is a polyethersulfone membran...

Embodiment 2

[0042] According to the preparation method of the above-mentioned biocatalytic conversion liquid, prepare 10 L of conversion liquid, wherein the content of UMP is 9.1 g / L.

[0043] The treatment process is as follows:

[0044] (1) After adjusting the pH value of the biocatalytic conversion liquid to 2.0 with hydrochloric acid, remove solid impurities after being centrifuged at 10,000 rpm for 20 minutes;

[0045] (2) the centrifuge liquid that step (1) is obtained is processed through ultrafiltration, and the ultrafiltration permeate is collected; the ultrafiltration membrane is a polyamide membrane, and the ultrafiltration membrane molecular weight cut-off is 5000, and the ultrafiltration membrane working pressure is 1MPa, the ultrafiltration temperature is 30°C, and the ultrafiltration pH value is 6.0.

[0046] (3) The ultrafiltration permeate obtained in step (2) is processed through nanofiltration, and the nanofiltration concentrate is collected; the nanofiltration membran...

Embodiment 3

[0050] According to the preparation method of the above-mentioned biocatalytic conversion liquid, prepare 10 L of conversion liquid, wherein the content of UMP is 8.1 g / L.

[0051] The treatment process is as follows:

[0052] (1) After adjusting the pH value of the biocatalytic conversion liquid to 3.0 with hydrochloric acid, remove solid impurities after being centrifuged at 10,000 rpm for 20 minutes;

[0053] (2) The centrifuge liquid that step (1) is obtained is processed through ultrafiltration, and the ultrafiltration permeate is collected; the ultrafiltration membrane is a polyamide membrane, and the ultrafiltration membrane molecular weight cut-off is 3000, and the ultrafiltration membrane working pressure is 1MPa, the ultrafiltration temperature is 30°C, and the ultrafiltration pH value is 7.0.

[0054] (3) The ultrafiltration permeate obtained in step (2) is processed through nanofiltration, and the nanofiltration concentrate is collected; the nanofiltration membran...

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PUM

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Abstract

The invention discloses a method for separating uridylic acid from biocatalytic conversion solution. The method conducts centrifugation, ultrafiltration, and nanofiltration to the biocatalytic conversion solution. Then, the conversion solution is moved to the anion-exchange column for absorption, deionized water washing, inorganic salt solution elution. The solution after elution is then nanofitrated to demineralise, concentrated and dried to obtain high-yielding, high-purity disodium uridine-5'-monophosphate crystal. The total separation yield rate can reach over 90 percent with the purity of 98 percent. The method of the invention lowers the consumption of resin, and the cost, but improves the yielding rate, the separation performance and the purity of the obtained product. The method is suitable for mass production.

Description

technical field [0001] The method relates to a production process of uridine acid, in particular to a method for separating uridine acid from a biocatalytic conversion liquid. Background technique [0002] Nucleotide is an important biochemical raw material, which can be used in food additives, pharmaceutical intermediates, feed additives, and plant growth promoters. Uridine acid (UMP) is a basic nucleotide and is a The most important kind of uridine acid, the market demand is very large, but the production of uridine acid is difficult, resulting in high prices. Most of the nucleotide production in my country is produced by enzymatic hydrolysis, which has a long production cycle, cumbersome process, and high difficulty in separation. Lead to high production cost, poor quality, low yield, and the purity cannot meet the production requirements such as downstream medicine. Biocatalytic nucleotide production technology is a new technology for nucleotide production, which has the ...

Claims

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Application Information

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IPC IPC(8): C07H19/10C07H1/06B01D15/36
Inventor 应汉杰金乃纯周熙群沈素芹苑巍熊健柏建新
Owner NANJING UNIV OF TECH
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