Application of Quzhazhigan in preparation of preparations for preventing and treating cardiac-cerebral ischemia diseases, and preparation method thereof
A technique for cardiovascular and cerebrovascular diseases and tristilbene, which is applied to the application of tristilbene in the preparation of preparations for preventing and treating cardio-cerebral ischemia and the field of preparation thereof, can solve the problems of reduced water solubility of compounds and the like, and achieves a simple and convenient process. Effect
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[0057] Example 1: Qualitative identification and quantitative detection of Lhasa rhubarb raw materials and stilbene extracts of stilbene
[0058] (1) Sample source
[0059] Wild Lhasa rhubarb rhizomes: purchased from Lhasa, Tibet, remove impurities, wash, cut into slices or pieces, and dry. Lot number: 20080913
[0060] Plant Lhasa rhubarb rhizomes: harvested in Shangri-La County, Yunnan, remove impurities, wash, cut into slices or pieces, and dry. Lot number: 20080915
[0061] Reference substance: Quza stilbene, homemade, purity 99%, batch number: 080602.
[0062] (2) Sample and standard solution preparation
[0063] Preparation of reference substance solution: accurately weigh out an appropriate amount of the reference substance of traza stilbene that has been vacuum-dried with phosphorus pentoxide, prepare 1mg / ml reference substance stock solution with methanol, and dilute it with 27% methanol-3% acetic acid solution to 25g / ml as Standard solution.
[0064] Preparation of Lhasa rhub...
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[0075] Example 2: Preparation of Triza stilbene extract
[0076] (1) Material: Lhasa rhubarb rhizomes: purchased from Lhasa, Tibet, remove impurities, wash, cut into slices or pieces, and dry. Lot number: 20080913
[0077] Resin: D101 macroporous adsorption resin, a product of Tianjin Haiguang Chemical Co., Ltd., pretreated according to the manufacturer's instructions.
[0078] (2) Preparation
[0079] i) Pretreatment of Lhasa rhubarb and determination of triza stilbene content:
[0080] The collected Lhasa rhubarb plants are removed from the mud and air-dried. The underground rhizomes and the above-ground stems and leaves are separated, and the pulverizer pulverizes them into coarse powder.
[0081] Take 100g each of the underground rhizome and the above ground stem and leaf powder, soak in 95% ethanol and reflux for extraction, 120 minutes / time, sampling at intervals for HPLC detection, until the extract is basically free of triza stilbene, and the extracts are combined according to E...
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[0105] Example 3: Preparation of pure stilbene from Lhasa rhubarb extract and stilbene extract
[0106] (1) Material: Lhasa rhubarb rhizomes: purchased from Lhasa, Tibet, remove impurities, wash, cut into slices or pieces, and dry. Lot number: 20080913.
[0107] (2) Preparation of Lhasa rhubarb extract: take 100g of Lhasa rhubarb dry rhizome powder, use 95% ethanol as the extraction solvent to reflux extract 3 times (2.4L / 1.8L / 1.2L), 120min / time, combine the extracts, concentrate, and vacuum Dry, Dersa rhubarb extract.
[0108] (3) Purification: 2 g of Lhasa rhubarb extract was mixed with 40 g of 200 mesh silica gel and purified by silica gel column chromatography. Using chloroform / methanol (V / V, 8 / 1) as the elution solvent, the different fractions were collected separately and monitored by thin layer chromatography (TLC). The fractions containing traza stilbene were combined and the solvent was recovered under reduced pressure. % Ethanol is recrystallized and dried in vacuum to o...
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